Experimental Lachesis muta rhombeata envenomation and effects of soursop (Annona muricata) as natural antivenom
Abstract Background In the Atlantic forest of the North and Northeast regions of Brazil, local population often uses the fruit juice and the aqueous extract of leaves of soursop (Annona muricata L.) to treat Lachesis muta rhombeata envenomation. Envenomation is a relevant health issue in these areas...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:a02de960ce284a2899cba9f4db27f0ab 2023-05-15T15:17:46+02:00 Experimental Lachesis muta rhombeata envenomation and effects of soursop (Annona muricata) as natural antivenom Caroline Marroni Cremonez Flávia Pine Leite Karla de Castro Figueiredo Bordon Felipe Augusto Cerni lara Aimê Cardoso Zita Maria de Oliveira Gregório Rodrigo Cançado Gonçalves de Souza Ana Maria de Souza Eliane Candiani Arantes 2016-04-01T00:00:00Z https://doi.org/10.1186/S40409-016-0067-6 https://doaj.org/article/a02de960ce284a2899cba9f4db27f0ab EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100310&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/S40409-016-0067-6 https://doaj.org/article/a02de960ce284a2899cba9f4db27f0ab Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 22, Iss 0 (2016) Lachesis muta rhombeata Bushmaster Natural antivenom Antiophidic action Soursop Annona muricata L Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2016 ftdoajarticles https://doi.org/10.1186/S40409-016-0067-6 2022-12-30T23:54:50Z Abstract Background In the Atlantic forest of the North and Northeast regions of Brazil, local population often uses the fruit juice and the aqueous extract of leaves of soursop (Annona muricata L.) to treat Lachesis muta rhombeata envenomation. Envenomation is a relevant health issue in these areas, especially due to its severity and because the production and distribution of antivenom is limited in these regions. The aim of the present study was to evaluate the relevance of the use of soursop leaf extract and its juice against envenomation by Lachesis muta rhombeata. Methods We evaluated the biochemical, hematological and hemostatic parameters, the blood pressure, the inflammation process and the lethality induced by Lachesis muta rhombeata snake venom. We also assessed the action of the aqueous extract of leaves (AmL) and juice (AmJ) from A. muricata on the animal organism injected with L. m. rhombeata venom (LmrV) in the laboratory environment. Results LmrV induced a decrease of total protein, albumin and glucose; and increase of creatine kinase, aspartate aminotransferase, and urea concentrations. It provoked hemoconcentration followed by reduction of hematocrit, an increase in prothrombin time and partial thromboplastin time and a decrease of the blood pressure. LmrV induced the release of interleukin-6, an increase in neutrophils and changes in the serum protein profile, characteristic of the acute inflammatory process. LD50 values were similar for the groups injected with LmrV and treated or untreated with AmJ and AmL. Both treatments play a role on the maintenance of blood glucose, urea and coagulation parameters and exert a protective action against the myotoxicity. However, they seem to worsen the hypotension caused by LmrV. Conclusion The treatments with AmJ and AmL present some beneficial actions, but they might intensify some effects of the venom. Therefore, additional studies on A. muricata are necessary to enable its use as natural antivenom for bushmaster snakebite. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 22 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Lachesis muta rhombeata Bushmaster Natural antivenom Antiophidic action Soursop Annona muricata L Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Lachesis muta rhombeata Bushmaster Natural antivenom Antiophidic action Soursop Annona muricata L Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Caroline Marroni Cremonez Flávia Pine Leite Karla de Castro Figueiredo Bordon Felipe Augusto Cerni lara Aimê Cardoso Zita Maria de Oliveira Gregório Rodrigo Cançado Gonçalves de Souza Ana Maria de Souza Eliane Candiani Arantes Experimental Lachesis muta rhombeata envenomation and effects of soursop (Annona muricata) as natural antivenom |
topic_facet |
Lachesis muta rhombeata Bushmaster Natural antivenom Antiophidic action Soursop Annona muricata L Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background In the Atlantic forest of the North and Northeast regions of Brazil, local population often uses the fruit juice and the aqueous extract of leaves of soursop (Annona muricata L.) to treat Lachesis muta rhombeata envenomation. Envenomation is a relevant health issue in these areas, especially due to its severity and because the production and distribution of antivenom is limited in these regions. The aim of the present study was to evaluate the relevance of the use of soursop leaf extract and its juice against envenomation by Lachesis muta rhombeata. Methods We evaluated the biochemical, hematological and hemostatic parameters, the blood pressure, the inflammation process and the lethality induced by Lachesis muta rhombeata snake venom. We also assessed the action of the aqueous extract of leaves (AmL) and juice (AmJ) from A. muricata on the animal organism injected with L. m. rhombeata venom (LmrV) in the laboratory environment. Results LmrV induced a decrease of total protein, albumin and glucose; and increase of creatine kinase, aspartate aminotransferase, and urea concentrations. It provoked hemoconcentration followed by reduction of hematocrit, an increase in prothrombin time and partial thromboplastin time and a decrease of the blood pressure. LmrV induced the release of interleukin-6, an increase in neutrophils and changes in the serum protein profile, characteristic of the acute inflammatory process. LD50 values were similar for the groups injected with LmrV and treated or untreated with AmJ and AmL. Both treatments play a role on the maintenance of blood glucose, urea and coagulation parameters and exert a protective action against the myotoxicity. However, they seem to worsen the hypotension caused by LmrV. Conclusion The treatments with AmJ and AmL present some beneficial actions, but they might intensify some effects of the venom. Therefore, additional studies on A. muricata are necessary to enable its use as natural antivenom for bushmaster snakebite. |
format |
Article in Journal/Newspaper |
author |
Caroline Marroni Cremonez Flávia Pine Leite Karla de Castro Figueiredo Bordon Felipe Augusto Cerni lara Aimê Cardoso Zita Maria de Oliveira Gregório Rodrigo Cançado Gonçalves de Souza Ana Maria de Souza Eliane Candiani Arantes |
author_facet |
Caroline Marroni Cremonez Flávia Pine Leite Karla de Castro Figueiredo Bordon Felipe Augusto Cerni lara Aimê Cardoso Zita Maria de Oliveira Gregório Rodrigo Cançado Gonçalves de Souza Ana Maria de Souza Eliane Candiani Arantes |
author_sort |
Caroline Marroni Cremonez |
title |
Experimental Lachesis muta rhombeata envenomation and effects of soursop (Annona muricata) as natural antivenom |
title_short |
Experimental Lachesis muta rhombeata envenomation and effects of soursop (Annona muricata) as natural antivenom |
title_full |
Experimental Lachesis muta rhombeata envenomation and effects of soursop (Annona muricata) as natural antivenom |
title_fullStr |
Experimental Lachesis muta rhombeata envenomation and effects of soursop (Annona muricata) as natural antivenom |
title_full_unstemmed |
Experimental Lachesis muta rhombeata envenomation and effects of soursop (Annona muricata) as natural antivenom |
title_sort |
experimental lachesis muta rhombeata envenomation and effects of soursop (annona muricata) as natural antivenom |
publisher |
SciELO |
publishDate |
2016 |
url |
https://doi.org/10.1186/S40409-016-0067-6 https://doaj.org/article/a02de960ce284a2899cba9f4db27f0ab |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 22, Iss 0 (2016) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100310&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/S40409-016-0067-6 https://doaj.org/article/a02de960ce284a2899cba9f4db27f0ab |
op_doi |
https://doi.org/10.1186/S40409-016-0067-6 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
22 |
container_issue |
1 |
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