Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a promising and reliable tool for arthropod identification, including the identification of alcohol-preserved ticks based on extracted leg protein spectra. In this study, the legs of 361...
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ftdoajarticles:oai:doaj.org/article:a02219298f5c4bbb8f76fe224385a5e3 2023-05-15T15:06:43+02:00 Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam. Ly Na Huynh Adama Zan Diarra Quang Luan Pham Nhiem Le-Viet Jean-Michel Berenger Van Hoang Ho Xuan Quang Nguyen Philippe Parola 2021-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009813 https://doaj.org/article/a02219298f5c4bbb8f76fe224385a5e3 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009813 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009813 https://doaj.org/article/a02219298f5c4bbb8f76fe224385a5e3 PLoS Neglected Tropical Diseases, Vol 15, Iss 9, p e0009813 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009813 2022-12-31T11:29:28Z Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a promising and reliable tool for arthropod identification, including the identification of alcohol-preserved ticks based on extracted leg protein spectra. In this study, the legs of 361 ticks collected in Vietnam, including 251 Rhiphicephalus sanguineus s.l, 99 Rhipicephalus (Boophilus) microplus, two Amblyomma varanensis, seven Dermacentor auratus, one Dermacentor compactus and one Amblyomma sp. were submitted for MALDI-TOF MS analyses. Spectral analysis showed intra-species reproducibility and inter-species specificity and the spectra of 329 (91%) specimens were of excellent quality. The blind test of 310 spectra remaining after updating the database with 19 spectra revealed that all were correctly identified with log score values (LSV) ranging from 1.7 to 2.396 with a mean of 1.982 ± 0.142 and a median of 1.971. The DNA of several microorganisms including Anaplasma platys, Anaplasma phagocytophilum, Anaplasma marginale, Ehrlichia rustica, Babesia vogeli, Theileria sinensis, and Theileria orientalis were detected in 25 ticks. Co-infection by A. phagocytophilum and T. sinensis was found in one Rh. (B) microplus. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 9 e0009813 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ly Na Huynh Adama Zan Diarra Quang Luan Pham Nhiem Le-Viet Jean-Michel Berenger Van Hoang Ho Xuan Quang Nguyen Philippe Parola Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a promising and reliable tool for arthropod identification, including the identification of alcohol-preserved ticks based on extracted leg protein spectra. In this study, the legs of 361 ticks collected in Vietnam, including 251 Rhiphicephalus sanguineus s.l, 99 Rhipicephalus (Boophilus) microplus, two Amblyomma varanensis, seven Dermacentor auratus, one Dermacentor compactus and one Amblyomma sp. were submitted for MALDI-TOF MS analyses. Spectral analysis showed intra-species reproducibility and inter-species specificity and the spectra of 329 (91%) specimens were of excellent quality. The blind test of 310 spectra remaining after updating the database with 19 spectra revealed that all were correctly identified with log score values (LSV) ranging from 1.7 to 2.396 with a mean of 1.982 ± 0.142 and a median of 1.971. The DNA of several microorganisms including Anaplasma platys, Anaplasma phagocytophilum, Anaplasma marginale, Ehrlichia rustica, Babesia vogeli, Theileria sinensis, and Theileria orientalis were detected in 25 ticks. Co-infection by A. phagocytophilum and T. sinensis was found in one Rh. (B) microplus. |
format |
Article in Journal/Newspaper |
author |
Ly Na Huynh Adama Zan Diarra Quang Luan Pham Nhiem Le-Viet Jean-Michel Berenger Van Hoang Ho Xuan Quang Nguyen Philippe Parola |
author_facet |
Ly Na Huynh Adama Zan Diarra Quang Luan Pham Nhiem Le-Viet Jean-Michel Berenger Van Hoang Ho Xuan Quang Nguyen Philippe Parola |
author_sort |
Ly Na Huynh |
title |
Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam. |
title_short |
Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam. |
title_full |
Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam. |
title_fullStr |
Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam. |
title_full_unstemmed |
Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam. |
title_sort |
morphological, molecular and maldi-tof ms identification of ticks and tick-associated pathogens in vietnam. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009813 https://doaj.org/article/a02219298f5c4bbb8f76fe224385a5e3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 9, p e0009813 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009813 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009813 https://doaj.org/article/a02219298f5c4bbb8f76fe224385a5e3 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009813 |
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PLOS Neglected Tropical Diseases |
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15 |
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9 |
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e0009813 |
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