A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding

We report a simple method for evaluating the binding of concanavalin A (ConA) to human peripheral blood mononuclear cells (PBMC). The binding is evidenced by an immunoenzymic assay using peroxidase-conjugated immunoglobulins of a rabbit anti-ConA serum. Using the method we show that sera from patien...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz
Main Authors: Manoel Barral-Netto, A. Barral
Format: Article in Journal/Newspaper
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 1986
Subjects:
concanavalin
mononuclear cells
visceral leishmaniasis
Arctic medicine. Tropical medicine
RC955-962
Microbiology
QR1-502
Arctic
Online Access:https://doi.org/10.1590/S0074-02761986000300012
https://doaj.org/article/a0037a2dc7a54e10986d617cb82c5f2f
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spelling ftdoajarticles:oai:doaj.org/article:a0037a2dc7a54e10986d617cb82c5f2f 2023-05-15T14:58:19+02:00 A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding Manoel Barral-Netto A. Barral 1986-09-01T00:00:00Z https://doi.org/10.1590/S0074-02761986000300012 https://doaj.org/article/a0037a2dc7a54e10986d617cb82c5f2f EN eng Instituto Oswaldo Cruz, Ministério da Saúde http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761986000300012 https://doaj.org/toc/0074-0276 https://doaj.org/toc/1678-8060 doi:10.1590/S0074-02761986000300012 0074-0276 1678-8060 https://doaj.org/article/a0037a2dc7a54e10986d617cb82c5f2f Memórias do Instituto Oswaldo Cruz., Vol 81, Iss 3, Pp 343-345 (1986) concanavalin mononuclear cells visceral leishmaniasis Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 article 1986 ftdoajarticles https://doi.org/10.1590/S0074-02761986000300012 2023-01-08T01:24:53Z We report a simple method for evaluating the binding of concanavalin A (ConA) to human peripheral blood mononuclear cells (PBMC). The binding is evidenced by an immunoenzymic assay using peroxidase-conjugated immunoglobulins of a rabbit anti-ConA serum. Using the method we show that sera from patients with American leishmaniasis do not interfere with binding of ConA to PBMC. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Memórias do Instituto Oswaldo Cruz 81 3 343 345
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic concanavalin
mononuclear cells
visceral leishmaniasis
Arctic medicine. Tropical medicine
RC955-962
Microbiology
QR1-502
spellingShingle concanavalin
mononuclear cells
visceral leishmaniasis
Arctic medicine. Tropical medicine
RC955-962
Microbiology
QR1-502
Manoel Barral-Netto
A. Barral
A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding
topic_facet concanavalin
mononuclear cells
visceral leishmaniasis
Arctic medicine. Tropical medicine
RC955-962
Microbiology
QR1-502
description We report a simple method for evaluating the binding of concanavalin A (ConA) to human peripheral blood mononuclear cells (PBMC). The binding is evidenced by an immunoenzymic assay using peroxidase-conjugated immunoglobulins of a rabbit anti-ConA serum. Using the method we show that sera from patients with American leishmaniasis do not interfere with binding of ConA to PBMC.
format Article in Journal/Newspaper
author Manoel Barral-Netto
A. Barral
author_facet Manoel Barral-Netto
A. Barral
author_sort Manoel Barral-Netto
title A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding
title_short A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding
title_full A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding
title_fullStr A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding
title_full_unstemmed A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding
title_sort simple method for assessing the binding of concanavalin a to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding
publisher Instituto Oswaldo Cruz, Ministério da Saúde
publishDate 1986
url https://doi.org/10.1590/S0074-02761986000300012
https://doaj.org/article/a0037a2dc7a54e10986d617cb82c5f2f
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source Memórias do Instituto Oswaldo Cruz., Vol 81, Iss 3, Pp 343-345 (1986)
op_relation http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761986000300012
https://doaj.org/toc/0074-0276
https://doaj.org/toc/1678-8060
doi:10.1590/S0074-02761986000300012
0074-0276
1678-8060
https://doaj.org/article/a0037a2dc7a54e10986d617cb82c5f2f
op_doi https://doi.org/10.1590/S0074-02761986000300012
container_title Memórias do Instituto Oswaldo Cruz
container_volume 81
container_issue 3
container_start_page 343
op_container_end_page 345
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