Development of genetic system to inactivate a Borrelia turicatae surface protein selectively produced within the salivary glands of the arthropod vector.
Borrelia turicatae, an agent of tick-borne relapsing fever, is an example of a pathogen that can adapt to disparate conditions found when colonizing the mammalian host and arthropod vector. However, little is known about the genetic factors necessary during the tick-mammalian infectious cycle, there...
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ftdoajarticles:oai:doaj.org/article:9ff316fd4cfc41fabd1ed756bdee9767 2023-05-15T15:09:25+02:00 Development of genetic system to inactivate a Borrelia turicatae surface protein selectively produced within the salivary glands of the arthropod vector. Job E Lopez Hannah K Wilder Reid Hargrove Christopher P Brooks Karin E Peterson Paul A Beare Daniel E Sturdevant Vijayaraj Nagarajan Sandra J Raffel Tom G Schwan 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002514 https://doaj.org/article/9ff316fd4cfc41fabd1ed756bdee9767 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3814808?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002514 https://doaj.org/article/9ff316fd4cfc41fabd1ed756bdee9767 PLoS Neglected Tropical Diseases, Vol 7, Iss 10, p e2514 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002514 2022-12-31T03:54:41Z Borrelia turicatae, an agent of tick-borne relapsing fever, is an example of a pathogen that can adapt to disparate conditions found when colonizing the mammalian host and arthropod vector. However, little is known about the genetic factors necessary during the tick-mammalian infectious cycle, therefore we developed a genetic system to transform this species of spirochete. We also identified a plasmid gene that was up-regulated in vitro when B. turicatae was grown in conditions mimicking the tick environment. This 40 kilodalton protein was predicted to be surface localized and designated the Borrelia repeat protein A (brpA) due to the redundancy of the amino acid motif Gln-Gly-Asn-Val-Glu.Quantitative reverse-transcriptase polymerase chain reaction using RNA from B. turicatae infected ticks and mice indicated differential regulation of brpA during the tick-mammalian infectious cycle. The surface localization was determined, and production of the protein within the salivary glands of the tick was demonstrated. We then applied a novel genetic system for B. turicatae to inactivate brpA and examined the role of the gene product for vector colonization and the ability to establish murine infection.These results demonstrate the complexity of protein production in a population of spirochetes within the tick. Additionally, the development of a genetic system is important for future studies to evaluate the requirement of specific B. turicatae genes for vector colonization and transmission. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 10 e2514 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Job E Lopez Hannah K Wilder Reid Hargrove Christopher P Brooks Karin E Peterson Paul A Beare Daniel E Sturdevant Vijayaraj Nagarajan Sandra J Raffel Tom G Schwan Development of genetic system to inactivate a Borrelia turicatae surface protein selectively produced within the salivary glands of the arthropod vector. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Borrelia turicatae, an agent of tick-borne relapsing fever, is an example of a pathogen that can adapt to disparate conditions found when colonizing the mammalian host and arthropod vector. However, little is known about the genetic factors necessary during the tick-mammalian infectious cycle, therefore we developed a genetic system to transform this species of spirochete. We also identified a plasmid gene that was up-regulated in vitro when B. turicatae was grown in conditions mimicking the tick environment. This 40 kilodalton protein was predicted to be surface localized and designated the Borrelia repeat protein A (brpA) due to the redundancy of the amino acid motif Gln-Gly-Asn-Val-Glu.Quantitative reverse-transcriptase polymerase chain reaction using RNA from B. turicatae infected ticks and mice indicated differential regulation of brpA during the tick-mammalian infectious cycle. The surface localization was determined, and production of the protein within the salivary glands of the tick was demonstrated. We then applied a novel genetic system for B. turicatae to inactivate brpA and examined the role of the gene product for vector colonization and the ability to establish murine infection.These results demonstrate the complexity of protein production in a population of spirochetes within the tick. Additionally, the development of a genetic system is important for future studies to evaluate the requirement of specific B. turicatae genes for vector colonization and transmission. |
format |
Article in Journal/Newspaper |
author |
Job E Lopez Hannah K Wilder Reid Hargrove Christopher P Brooks Karin E Peterson Paul A Beare Daniel E Sturdevant Vijayaraj Nagarajan Sandra J Raffel Tom G Schwan |
author_facet |
Job E Lopez Hannah K Wilder Reid Hargrove Christopher P Brooks Karin E Peterson Paul A Beare Daniel E Sturdevant Vijayaraj Nagarajan Sandra J Raffel Tom G Schwan |
author_sort |
Job E Lopez |
title |
Development of genetic system to inactivate a Borrelia turicatae surface protein selectively produced within the salivary glands of the arthropod vector. |
title_short |
Development of genetic system to inactivate a Borrelia turicatae surface protein selectively produced within the salivary glands of the arthropod vector. |
title_full |
Development of genetic system to inactivate a Borrelia turicatae surface protein selectively produced within the salivary glands of the arthropod vector. |
title_fullStr |
Development of genetic system to inactivate a Borrelia turicatae surface protein selectively produced within the salivary glands of the arthropod vector. |
title_full_unstemmed |
Development of genetic system to inactivate a Borrelia turicatae surface protein selectively produced within the salivary glands of the arthropod vector. |
title_sort |
development of genetic system to inactivate a borrelia turicatae surface protein selectively produced within the salivary glands of the arthropod vector. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002514 https://doaj.org/article/9ff316fd4cfc41fabd1ed756bdee9767 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 10, p e2514 (2013) |
op_relation |
http://europepmc.org/articles/PMC3814808?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002514 https://doaj.org/article/9ff316fd4cfc41fabd1ed756bdee9767 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002514 |
container_title |
PLoS Neglected Tropical Diseases |
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7 |
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10 |
container_start_page |
e2514 |
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1766340612451205120 |