New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves
ABSTRACT The signs of climate change are undeniable, and the impact of these changes on ecosystem function heavily depends on the response of microbes that underpin the food web. Antarctic ice shelf is a massive mass of floating ice that extends from the continent into the ocean, exerting a profound...
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American Society for Microbiology
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ftdoajarticles:oai:doaj.org/article:9f967d9d3111465a92bd1848137a7e90 2024-09-15T17:48:20+00:00 New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves Aitana Llorenç-Vicedo Monica Lluesma Gomez Ole Zeising Thomas Kleiner Johannes Freitag Francisco Martinez-Hernandez Frank Wilhelms Manuel Martinez-Garcia 2024-05-01T00:00:00Z https://doi.org/10.1128/msphere.00073-24 https://doaj.org/article/9f967d9d3111465a92bd1848137a7e90 EN eng American Society for Microbiology https://journals.asm.org/doi/10.1128/msphere.00073-24 https://doaj.org/toc/2379-5042 doi:10.1128/msphere.00073-24 2379-5042 https://doaj.org/article/9f967d9d3111465a92bd1848137a7e90 mSphere, Vol 9, Iss 5 (2024) Antarctic ice shelf single-cell genomics marine ice microbiome bacteria Microbiology QR1-502 article 2024 ftdoajarticles https://doi.org/10.1128/msphere.00073-24 2024-08-05T17:49:18Z ABSTRACT The signs of climate change are undeniable, and the impact of these changes on ecosystem function heavily depends on the response of microbes that underpin the food web. Antarctic ice shelf is a massive mass of floating ice that extends from the continent into the ocean, exerting a profound influence on global carbon cycles. Beneath Antarctic ice shelves, marine ice stores valuable genetic information, where marine microbial communities before the industrial revolution are archived. Here, in this proof-of-concept, by employing a combination of single-cell technologiesand metagenomics, we have been able to sequence frozen microbial DNA (≈300 years old) stored in the marine ice core B15 collected from the Filchnner-Ronne Ice Shelf. Metagenomic data indicated that Proteobacteria and Thaumarchaeota (e.g., Nitrosopumilus spp.), followed by Actinobacteria (e.g., Actinomarinales), were abundant. Remarkably, our data allow us to “travel to the past” and calibrate genomic and genetic evolutionary changes for ecologically relevant microbes and functions, such as Nitrosopumilus spp., preserved in the marine ice (≈300 years old) with those collected recently in seawater under an ice shelf (year 2017). The evolutionary divergence for the ammonia monooxygenase gene amoA involved in chemolithoautotrophy was about 0.88 amino acid and 2.8 nucleotide substitution rate per 100 sites in a century, while the accumulated rate of genomic SNPs was 2,467 per 1 Mb of genome and 100 years. Whether these evolutionary changes remained constant over the last 300 years or accelerated during post-industrial periods remains an open question that will be further elucidated.IMPORTANCESeveral efforts have been undertaken to predict the response of microbes under climate change, mainly based on short-term microcosm experiments under forced conditions. A common concern is that manipulative experiments cannot properly simulate the response of microbes to climate change, which is a long-term evolutionary process. In this proof-of-concept ... Article in Journal/Newspaper Antarc* Antarctic ice core Ice Shelf Ice Shelves Ronne Ice Shelf Directory of Open Access Journals: DOAJ Articles mSphere 9 5 |
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topic |
Antarctic ice shelf single-cell genomics marine ice microbiome bacteria Microbiology QR1-502 |
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Antarctic ice shelf single-cell genomics marine ice microbiome bacteria Microbiology QR1-502 Aitana Llorenç-Vicedo Monica Lluesma Gomez Ole Zeising Thomas Kleiner Johannes Freitag Francisco Martinez-Hernandez Frank Wilhelms Manuel Martinez-Garcia New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves |
topic_facet |
Antarctic ice shelf single-cell genomics marine ice microbiome bacteria Microbiology QR1-502 |
description |
ABSTRACT The signs of climate change are undeniable, and the impact of these changes on ecosystem function heavily depends on the response of microbes that underpin the food web. Antarctic ice shelf is a massive mass of floating ice that extends from the continent into the ocean, exerting a profound influence on global carbon cycles. Beneath Antarctic ice shelves, marine ice stores valuable genetic information, where marine microbial communities before the industrial revolution are archived. Here, in this proof-of-concept, by employing a combination of single-cell technologiesand metagenomics, we have been able to sequence frozen microbial DNA (≈300 years old) stored in the marine ice core B15 collected from the Filchnner-Ronne Ice Shelf. Metagenomic data indicated that Proteobacteria and Thaumarchaeota (e.g., Nitrosopumilus spp.), followed by Actinobacteria (e.g., Actinomarinales), were abundant. Remarkably, our data allow us to “travel to the past” and calibrate genomic and genetic evolutionary changes for ecologically relevant microbes and functions, such as Nitrosopumilus spp., preserved in the marine ice (≈300 years old) with those collected recently in seawater under an ice shelf (year 2017). The evolutionary divergence for the ammonia monooxygenase gene amoA involved in chemolithoautotrophy was about 0.88 amino acid and 2.8 nucleotide substitution rate per 100 sites in a century, while the accumulated rate of genomic SNPs was 2,467 per 1 Mb of genome and 100 years. Whether these evolutionary changes remained constant over the last 300 years or accelerated during post-industrial periods remains an open question that will be further elucidated.IMPORTANCESeveral efforts have been undertaken to predict the response of microbes under climate change, mainly based on short-term microcosm experiments under forced conditions. A common concern is that manipulative experiments cannot properly simulate the response of microbes to climate change, which is a long-term evolutionary process. In this proof-of-concept ... |
format |
Article in Journal/Newspaper |
author |
Aitana Llorenç-Vicedo Monica Lluesma Gomez Ole Zeising Thomas Kleiner Johannes Freitag Francisco Martinez-Hernandez Frank Wilhelms Manuel Martinez-Garcia |
author_facet |
Aitana Llorenç-Vicedo Monica Lluesma Gomez Ole Zeising Thomas Kleiner Johannes Freitag Francisco Martinez-Hernandez Frank Wilhelms Manuel Martinez-Garcia |
author_sort |
Aitana Llorenç-Vicedo |
title |
New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves |
title_short |
New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves |
title_full |
New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves |
title_fullStr |
New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves |
title_full_unstemmed |
New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves |
title_sort |
new avenues for potentially seeking microbial responses to climate change beneath antarctic ice shelves |
publisher |
American Society for Microbiology |
publishDate |
2024 |
url |
https://doi.org/10.1128/msphere.00073-24 https://doaj.org/article/9f967d9d3111465a92bd1848137a7e90 |
genre |
Antarc* Antarctic ice core Ice Shelf Ice Shelves Ronne Ice Shelf |
genre_facet |
Antarc* Antarctic ice core Ice Shelf Ice Shelves Ronne Ice Shelf |
op_source |
mSphere, Vol 9, Iss 5 (2024) |
op_relation |
https://journals.asm.org/doi/10.1128/msphere.00073-24 https://doaj.org/toc/2379-5042 doi:10.1128/msphere.00073-24 2379-5042 https://doaj.org/article/9f967d9d3111465a92bd1848137a7e90 |
op_doi |
https://doi.org/10.1128/msphere.00073-24 |
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mSphere |
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9 |
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5 |
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1810289477766086656 |