Chemical constituents, in vitro antimicrobial and cytotoxic potentials of the extracts from Macaranga barteri Mull-Arg
Objectives: To investigate antimicrobial and cytotoxic potentials as well as chemical constituents of extracts from Macaranga barteri (M. barteri). Methods: Antimicrobial activity was carried out using micro-dilution, cell culture and GC–MS methods were employed to determine the cytotoxicity and che...
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Wolters Kluwer Medknow Publications
2017
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ftdoajarticles:oai:doaj.org/article:9f8c5e200c5d4716b37b1914179a1da3 2023-05-15T15:09:54+02:00 Chemical constituents, in vitro antimicrobial and cytotoxic potentials of the extracts from Macaranga barteri Mull-Arg Akintayo Ogundajo Benjamin Okeleye Anofi Omotayo Ashafa 2017-07-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2017.06.014 https://doaj.org/article/9f8c5e200c5d4716b37b1914179a1da3 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169117306202 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.06.014 https://doaj.org/article/9f8c5e200c5d4716b37b1914179a1da3 Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 7, Pp 654-659 (2017) Macaranga barteri GC–MS Antimicrobial Cytotoxicity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2017.06.014 2022-12-31T12:31:19Z Objectives: To investigate antimicrobial and cytotoxic potentials as well as chemical constituents of extracts from Macaranga barteri (M. barteri). Methods: Antimicrobial activity was carried out using micro-dilution, cell culture and GC–MS methods were employed to determine the cytotoxicity and chemical constituents of the extracts respectively. Results: Marked activity was observed in methanol (ME) fraction [MIC50: (0.0977–6.2500) mg/mL] compared to hexane and ethyl acetate fractions. Aeromonas hydrophila (environmental strain) and Shigella sonnei (ATCC 29930) were the most susceptible pathogens to ME and ciprofloxacin (Cl) at MIC50 value of 0.0977 and < 0.0195 mg/mL respectively. Cryptococcus neoformans (ATCC 66031) was susceptible to ME at 0.1953 mg/mL compared to fluconazole at 10.0000 μg/mL. Decreased viability of the Vero cells was observed at the concentrations of 0.1–1.0 mg/mL. The lethal dose (LC50) of hexane, ethyl acetate and methanol fractions were recorded at (0.30 ± 0.07), (0.52 ± 0.05) and (0.22 ± 0.04) mg/mL, respectively. Some of the compounds identified from ME were caryophyllene (25.21%), neophytadiene (11.90%), α-humulene (7.67%), phytol (4.40%), ethyl ester hexadecanoic acid (4.04%) and nerolidol (2.83%) which were known to have various antimicrobial activities. Conclusions: Methanol fraction of M. barteri is a potent and safe antimicrobial and antifungal alternative which can be useful in the search for new antimicrobial drugs. The study also confirmed the orthodox usage of M. barteri in combating infectious diseases. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Mull ENVELOPE(-63.058,-63.058,-74.536,-74.536) Asian Pacific Journal of Tropical Biomedicine 7 7 654 659 |
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op_collection_id |
ftdoajarticles |
language |
English |
topic |
Macaranga barteri GC–MS Antimicrobial Cytotoxicity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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Macaranga barteri GC–MS Antimicrobial Cytotoxicity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Akintayo Ogundajo Benjamin Okeleye Anofi Omotayo Ashafa Chemical constituents, in vitro antimicrobial and cytotoxic potentials of the extracts from Macaranga barteri Mull-Arg |
topic_facet |
Macaranga barteri GC–MS Antimicrobial Cytotoxicity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objectives: To investigate antimicrobial and cytotoxic potentials as well as chemical constituents of extracts from Macaranga barteri (M. barteri). Methods: Antimicrobial activity was carried out using micro-dilution, cell culture and GC–MS methods were employed to determine the cytotoxicity and chemical constituents of the extracts respectively. Results: Marked activity was observed in methanol (ME) fraction [MIC50: (0.0977–6.2500) mg/mL] compared to hexane and ethyl acetate fractions. Aeromonas hydrophila (environmental strain) and Shigella sonnei (ATCC 29930) were the most susceptible pathogens to ME and ciprofloxacin (Cl) at MIC50 value of 0.0977 and < 0.0195 mg/mL respectively. Cryptococcus neoformans (ATCC 66031) was susceptible to ME at 0.1953 mg/mL compared to fluconazole at 10.0000 μg/mL. Decreased viability of the Vero cells was observed at the concentrations of 0.1–1.0 mg/mL. The lethal dose (LC50) of hexane, ethyl acetate and methanol fractions were recorded at (0.30 ± 0.07), (0.52 ± 0.05) and (0.22 ± 0.04) mg/mL, respectively. Some of the compounds identified from ME were caryophyllene (25.21%), neophytadiene (11.90%), α-humulene (7.67%), phytol (4.40%), ethyl ester hexadecanoic acid (4.04%) and nerolidol (2.83%) which were known to have various antimicrobial activities. Conclusions: Methanol fraction of M. barteri is a potent and safe antimicrobial and antifungal alternative which can be useful in the search for new antimicrobial drugs. The study also confirmed the orthodox usage of M. barteri in combating infectious diseases. |
format |
Article in Journal/Newspaper |
author |
Akintayo Ogundajo Benjamin Okeleye Anofi Omotayo Ashafa |
author_facet |
Akintayo Ogundajo Benjamin Okeleye Anofi Omotayo Ashafa |
author_sort |
Akintayo Ogundajo |
title |
Chemical constituents, in vitro antimicrobial and cytotoxic potentials of the extracts from Macaranga barteri Mull-Arg |
title_short |
Chemical constituents, in vitro antimicrobial and cytotoxic potentials of the extracts from Macaranga barteri Mull-Arg |
title_full |
Chemical constituents, in vitro antimicrobial and cytotoxic potentials of the extracts from Macaranga barteri Mull-Arg |
title_fullStr |
Chemical constituents, in vitro antimicrobial and cytotoxic potentials of the extracts from Macaranga barteri Mull-Arg |
title_full_unstemmed |
Chemical constituents, in vitro antimicrobial and cytotoxic potentials of the extracts from Macaranga barteri Mull-Arg |
title_sort |
chemical constituents, in vitro antimicrobial and cytotoxic potentials of the extracts from macaranga barteri mull-arg |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2017.06.014 https://doaj.org/article/9f8c5e200c5d4716b37b1914179a1da3 |
long_lat |
ENVELOPE(-63.058,-63.058,-74.536,-74.536) |
geographic |
Arctic Mull |
geographic_facet |
Arctic Mull |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 7, Pp 654-659 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169117306202 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.06.014 https://doaj.org/article/9f8c5e200c5d4716b37b1914179a1da3 |
op_doi |
https://doi.org/10.1016/j.apjtb.2017.06.014 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
7 |
container_start_page |
654 |
op_container_end_page |
659 |
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1766341001766502400 |