Surveillance of genetic markers associated with Plasmodium falciparum resistance to artemisinin-based combination therapy in Pakistan, 2018–2019
Abstract Background The spread of artemisinin resistance in the Greater Mekong Subregion of Southeast Asia poses a significant threat for current anti-malarial treatment guidelines globally. The aim of this study was to assess the current prevalence of molecular markers of drug resistance in Plasmod...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2020
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s12936-020-03276-8 https://doaj.org/article/9f457b3cb3c540f8ae540cce07f1a785 |
id |
ftdoajarticles:oai:doaj.org/article:9f457b3cb3c540f8ae540cce07f1a785 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:9f457b3cb3c540f8ae540cce07f1a785 2023-05-15T15:14:47+02:00 Surveillance of genetic markers associated with Plasmodium falciparum resistance to artemisinin-based combination therapy in Pakistan, 2018–2019 Abdul Qader Khan Leyre Pernaute-Lau Aamer Ali Khattak Sanna Luijcx Berit Aydin-Schmidt Mubashir Hussain Taj Ali Khan Farees Uddin Mufti Ulrika Morris 2020-06-01T00:00:00Z https://doi.org/10.1186/s12936-020-03276-8 https://doaj.org/article/9f457b3cb3c540f8ae540cce07f1a785 EN eng BMC http://link.springer.com/article/10.1186/s12936-020-03276-8 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03276-8 1475-2875 https://doaj.org/article/9f457b3cb3c540f8ae540cce07f1a785 Malaria Journal, Vol 19, Iss 1, Pp 1-9 (2020) Plasmodium falciparum Pakistan Artemisinin-based combination therapy Artemether–lumefantrine Chloroquine Drug resistance Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-03276-8 2022-12-31T09:29:24Z Abstract Background The spread of artemisinin resistance in the Greater Mekong Subregion of Southeast Asia poses a significant threat for current anti-malarial treatment guidelines globally. The aim of this study was to assess the current prevalence of molecular markers of drug resistance in Plasmodium falciparum in the four provinces with the highest malaria burden in Pakistan, after introducing artemether–lumefantrine as first-line treatment in 2017. Methods Samples were collected during routine malaria surveillance in Punjab, Sindh, Baluchistan, and Khyber Pakhtunkhwa provinces of Pakistan between January 2018 and February 2019. Plasmodium falciparum infections were confirmed by rapid diagnostic test or microscopy. Plasmodium falciparum positive isolates (n = 179) were screened by Sanger sequencing for single nucleotide polymorphisms (SNPs) in the P . falciparum kelch 13 (pfk13) propeller domain and in P. falciparum coronin (pfcoronin). SNPs in P. falciparum multidrug resistance 1 (pfmdr1) N86Y, Y184F, D1246Y and P. falciparum chloroquine resistance transporter (pfcrt) K76T were genotyped by PCR-restriction fragment length polymorphism. Results No artemisinin resistance associated SNPs were identified in the pfk13 propeller domain or in pfcoronin. The pfmdr1 N86, 184F, D1246 and pfcrt K76 alleles associated with reduced lumefantrine sensitivity were present in 83.8% (150/179), 16.9% (29/172), 100.0% (173/173), and 8.4% (15/179) of all infections, respectively. The chloroquine resistance associated pfcrt 76T allele was present in 98.3% (176/179) of infections. Conclusion This study provides an update on the current prevalence of molecular markers associated with reduced P. falciparum sensitivity to artemether and/or lumefantrine in Pakistan, including a first baseline assessment of polymorphisms in pfcoronin. No mutations associated with artemisinin resistance were observed in pfk13 or pfcoronin. However, the prevalence of the pfmdr1 N86 and D1246 alleles, that have been associated with decreased ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Plasmodium falciparum Pakistan Artemisinin-based combination therapy Artemether–lumefantrine Chloroquine Drug resistance Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Plasmodium falciparum Pakistan Artemisinin-based combination therapy Artemether–lumefantrine Chloroquine Drug resistance Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Abdul Qader Khan Leyre Pernaute-Lau Aamer Ali Khattak Sanna Luijcx Berit Aydin-Schmidt Mubashir Hussain Taj Ali Khan Farees Uddin Mufti Ulrika Morris Surveillance of genetic markers associated with Plasmodium falciparum resistance to artemisinin-based combination therapy in Pakistan, 2018–2019 |
topic_facet |
Plasmodium falciparum Pakistan Artemisinin-based combination therapy Artemether–lumefantrine Chloroquine Drug resistance Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The spread of artemisinin resistance in the Greater Mekong Subregion of Southeast Asia poses a significant threat for current anti-malarial treatment guidelines globally. The aim of this study was to assess the current prevalence of molecular markers of drug resistance in Plasmodium falciparum in the four provinces with the highest malaria burden in Pakistan, after introducing artemether–lumefantrine as first-line treatment in 2017. Methods Samples were collected during routine malaria surveillance in Punjab, Sindh, Baluchistan, and Khyber Pakhtunkhwa provinces of Pakistan between January 2018 and February 2019. Plasmodium falciparum infections were confirmed by rapid diagnostic test or microscopy. Plasmodium falciparum positive isolates (n = 179) were screened by Sanger sequencing for single nucleotide polymorphisms (SNPs) in the P . falciparum kelch 13 (pfk13) propeller domain and in P. falciparum coronin (pfcoronin). SNPs in P. falciparum multidrug resistance 1 (pfmdr1) N86Y, Y184F, D1246Y and P. falciparum chloroquine resistance transporter (pfcrt) K76T were genotyped by PCR-restriction fragment length polymorphism. Results No artemisinin resistance associated SNPs were identified in the pfk13 propeller domain or in pfcoronin. The pfmdr1 N86, 184F, D1246 and pfcrt K76 alleles associated with reduced lumefantrine sensitivity were present in 83.8% (150/179), 16.9% (29/172), 100.0% (173/173), and 8.4% (15/179) of all infections, respectively. The chloroquine resistance associated pfcrt 76T allele was present in 98.3% (176/179) of infections. Conclusion This study provides an update on the current prevalence of molecular markers associated with reduced P. falciparum sensitivity to artemether and/or lumefantrine in Pakistan, including a first baseline assessment of polymorphisms in pfcoronin. No mutations associated with artemisinin resistance were observed in pfk13 or pfcoronin. However, the prevalence of the pfmdr1 N86 and D1246 alleles, that have been associated with decreased ... |
format |
Article in Journal/Newspaper |
author |
Abdul Qader Khan Leyre Pernaute-Lau Aamer Ali Khattak Sanna Luijcx Berit Aydin-Schmidt Mubashir Hussain Taj Ali Khan Farees Uddin Mufti Ulrika Morris |
author_facet |
Abdul Qader Khan Leyre Pernaute-Lau Aamer Ali Khattak Sanna Luijcx Berit Aydin-Schmidt Mubashir Hussain Taj Ali Khan Farees Uddin Mufti Ulrika Morris |
author_sort |
Abdul Qader Khan |
title |
Surveillance of genetic markers associated with Plasmodium falciparum resistance to artemisinin-based combination therapy in Pakistan, 2018–2019 |
title_short |
Surveillance of genetic markers associated with Plasmodium falciparum resistance to artemisinin-based combination therapy in Pakistan, 2018–2019 |
title_full |
Surveillance of genetic markers associated with Plasmodium falciparum resistance to artemisinin-based combination therapy in Pakistan, 2018–2019 |
title_fullStr |
Surveillance of genetic markers associated with Plasmodium falciparum resistance to artemisinin-based combination therapy in Pakistan, 2018–2019 |
title_full_unstemmed |
Surveillance of genetic markers associated with Plasmodium falciparum resistance to artemisinin-based combination therapy in Pakistan, 2018–2019 |
title_sort |
surveillance of genetic markers associated with plasmodium falciparum resistance to artemisinin-based combination therapy in pakistan, 2018–2019 |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-03276-8 https://doaj.org/article/9f457b3cb3c540f8ae540cce07f1a785 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-9 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12936-020-03276-8 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03276-8 1475-2875 https://doaj.org/article/9f457b3cb3c540f8ae540cce07f1a785 |
op_doi |
https://doi.org/10.1186/s12936-020-03276-8 |
container_title |
Malaria Journal |
container_volume |
19 |
container_issue |
1 |
_version_ |
1766345202448990208 |