Point-of-care testing for Toxoplasma gondii IgG/IgM using Toxoplasma ICT IgG-IgM test with sera from the United States and implications for developing countries.
BACKGROUND:Congenital toxoplasmosis is a serious but preventable and treatable disease. Gestational screening facilitates early detection and treatment of primary acquisition. Thus, fetal infection can be promptly diagnosed and treated and outcomes can be improved. METHODS:We tested 180 sera with th...
Published in: | PLOS Neglected Tropical Diseases |
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Online Access: | https://doi.org/10.1371/journal.pntd.0005670 https://doaj.org/article/9f2617e55968471c9b68fa50a0fa5d9c |
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ftdoajarticles:oai:doaj.org/article:9f2617e55968471c9b68fa50a0fa5d9c 2023-05-15T15:12:08+02:00 Point-of-care testing for Toxoplasma gondii IgG/IgM using Toxoplasma ICT IgG-IgM test with sera from the United States and implications for developing countries. Ian J Begeman Joseph Lykins Ying Zhou Bo Shiun Lai Pauline Levigne Kamal El Bissati Kenneth Boyer Shawn Withers Fatima Clouser A Gwendolyn Noble Peter Rabiah Charles N Swisher Peter T Heydemann Despina G Contopoulos-Ioannidis Jose G Montoya Yvonne Maldonado Raymund Ramirez Cindy Press Eileen Stillwaggon François Peyron Rima McLeod 2017-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005670 https://doaj.org/article/9f2617e55968471c9b68fa50a0fa5d9c EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5501679?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005670 https://doaj.org/article/9f2617e55968471c9b68fa50a0fa5d9c PLoS Neglected Tropical Diseases, Vol 11, Iss 6, p e0005670 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005670 2022-12-31T10:59:14Z BACKGROUND:Congenital toxoplasmosis is a serious but preventable and treatable disease. Gestational screening facilitates early detection and treatment of primary acquisition. Thus, fetal infection can be promptly diagnosed and treated and outcomes can be improved. METHODS:We tested 180 sera with the Toxoplasma ICT IgG-IgM point-of-care (POC) test. Sera were from 116 chronically infected persons (48 serotype II; 14 serotype I-III; 25 serotype I-IIIa; 28 serotype Atypical, haplogroup 12; 1 not typed). These represent strains of parasites infecting mothers of congenitally infected children in the U.S. 51 seronegative samples and 13 samples from recently infected persons known to be IgG/IgM positive within the prior 2.7 months also were tested. Interpretation was confirmed by two blinded observers. A comparison of costs for POC vs. commercial laboratory testing methods was performed. RESULTS:We found that this new Toxoplasma ICT IgG-IgM POC test was highly sensitive (100%) and specific (100%) for distinguishing IgG/IgM-positive from negative sera. Use of such reliable POC tests can be cost-saving and benefit patients. CONCLUSIONS:Our work demonstrates that the Toxoplasma ICT IgG-IgM test can function reliably as a point-of-care test to diagnose Toxoplasma gondii infection in the U.S. This provides an opportunity to improve maternal-fetal care by using approaches, diagnostic tools, and medicines already available. This infection has serious, lifelong consequences for infected persons and their families. From the present study, it appears a simple, low-cost POC test is now available to help prevent morbidity/disability, decrease cost, and make gestational screening feasible. It also offers new options for improved prenatal care in low- and middle-income countries. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 6 e0005670 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ian J Begeman Joseph Lykins Ying Zhou Bo Shiun Lai Pauline Levigne Kamal El Bissati Kenneth Boyer Shawn Withers Fatima Clouser A Gwendolyn Noble Peter Rabiah Charles N Swisher Peter T Heydemann Despina G Contopoulos-Ioannidis Jose G Montoya Yvonne Maldonado Raymund Ramirez Cindy Press Eileen Stillwaggon François Peyron Rima McLeod Point-of-care testing for Toxoplasma gondii IgG/IgM using Toxoplasma ICT IgG-IgM test with sera from the United States and implications for developing countries. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Congenital toxoplasmosis is a serious but preventable and treatable disease. Gestational screening facilitates early detection and treatment of primary acquisition. Thus, fetal infection can be promptly diagnosed and treated and outcomes can be improved. METHODS:We tested 180 sera with the Toxoplasma ICT IgG-IgM point-of-care (POC) test. Sera were from 116 chronically infected persons (48 serotype II; 14 serotype I-III; 25 serotype I-IIIa; 28 serotype Atypical, haplogroup 12; 1 not typed). These represent strains of parasites infecting mothers of congenitally infected children in the U.S. 51 seronegative samples and 13 samples from recently infected persons known to be IgG/IgM positive within the prior 2.7 months also were tested. Interpretation was confirmed by two blinded observers. A comparison of costs for POC vs. commercial laboratory testing methods was performed. RESULTS:We found that this new Toxoplasma ICT IgG-IgM POC test was highly sensitive (100%) and specific (100%) for distinguishing IgG/IgM-positive from negative sera. Use of such reliable POC tests can be cost-saving and benefit patients. CONCLUSIONS:Our work demonstrates that the Toxoplasma ICT IgG-IgM test can function reliably as a point-of-care test to diagnose Toxoplasma gondii infection in the U.S. This provides an opportunity to improve maternal-fetal care by using approaches, diagnostic tools, and medicines already available. This infection has serious, lifelong consequences for infected persons and their families. From the present study, it appears a simple, low-cost POC test is now available to help prevent morbidity/disability, decrease cost, and make gestational screening feasible. It also offers new options for improved prenatal care in low- and middle-income countries. |
format |
Article in Journal/Newspaper |
author |
Ian J Begeman Joseph Lykins Ying Zhou Bo Shiun Lai Pauline Levigne Kamal El Bissati Kenneth Boyer Shawn Withers Fatima Clouser A Gwendolyn Noble Peter Rabiah Charles N Swisher Peter T Heydemann Despina G Contopoulos-Ioannidis Jose G Montoya Yvonne Maldonado Raymund Ramirez Cindy Press Eileen Stillwaggon François Peyron Rima McLeod |
author_facet |
Ian J Begeman Joseph Lykins Ying Zhou Bo Shiun Lai Pauline Levigne Kamal El Bissati Kenneth Boyer Shawn Withers Fatima Clouser A Gwendolyn Noble Peter Rabiah Charles N Swisher Peter T Heydemann Despina G Contopoulos-Ioannidis Jose G Montoya Yvonne Maldonado Raymund Ramirez Cindy Press Eileen Stillwaggon François Peyron Rima McLeod |
author_sort |
Ian J Begeman |
title |
Point-of-care testing for Toxoplasma gondii IgG/IgM using Toxoplasma ICT IgG-IgM test with sera from the United States and implications for developing countries. |
title_short |
Point-of-care testing for Toxoplasma gondii IgG/IgM using Toxoplasma ICT IgG-IgM test with sera from the United States and implications for developing countries. |
title_full |
Point-of-care testing for Toxoplasma gondii IgG/IgM using Toxoplasma ICT IgG-IgM test with sera from the United States and implications for developing countries. |
title_fullStr |
Point-of-care testing for Toxoplasma gondii IgG/IgM using Toxoplasma ICT IgG-IgM test with sera from the United States and implications for developing countries. |
title_full_unstemmed |
Point-of-care testing for Toxoplasma gondii IgG/IgM using Toxoplasma ICT IgG-IgM test with sera from the United States and implications for developing countries. |
title_sort |
point-of-care testing for toxoplasma gondii igg/igm using toxoplasma ict igg-igm test with sera from the united states and implications for developing countries. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005670 https://doaj.org/article/9f2617e55968471c9b68fa50a0fa5d9c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 6, p e0005670 (2017) |
op_relation |
http://europepmc.org/articles/PMC5501679?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005670 https://doaj.org/article/9f2617e55968471c9b68fa50a0fa5d9c |
op_doi |
https://doi.org/10.1371/journal.pntd.0005670 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
6 |
container_start_page |
e0005670 |
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