A COMPARISON OF THE PROTEIN PROFILE OF TRIAENOPHORUS NODULOSUS PLEROCERCOIDS FROM DIFFERENT INTERMEDIATE HOSTS

• Published in: Proceedings of the Karelian Research Centre of the Russian Academy of Sciences
• Main Authors: Albina Kochneva, Ekaterina Borvinskaya, Darya Bedulina, Irina Sukhovskaya
• Format: Article in Journal/Newspaper
• Language: English; Russian
• Published: Karelian Research Centre of the Russian Academy of Sciences 2018
• Subjects: proteomics; helminths; triaenophorus; cestoda; intermediate hosts; Science; Q; Burbot; Lota lota; Gymnocephalus cernuus; lota
• Online Access: https://doi.org/10.17076/eb899; https://doaj.org/article/9f0702bd9bc348308d9d1b369439bd90

The spectrum of proteins was studied in plerocercoids of parasitic cestodes Triaenophorus nodulosus collected from the liver of different species of fish: perch Perca fluviatilis L ., ruffe Gymnocephalus cernuus L. and burbot Lota lota L. A comparison of protein extracts separated by 2D electrophoresis revealed 18 proteins whose concentrations differed among larvae of different fish species. Of these, 4 proteins were identified by using mass-spectrometric methods (MALDI-TOF/TOF, LC–MS): a carbohydrate metabolism enzyme triosephosphate isomerase, the proteins of microtubules tubulin α and β, and the G-protein signaling molecule. The elevated content of components of the G-protein pathway of the cytoskeleton and cell morphology regulation (tubulin and G-protein) in plerocercoids from perch in comparison with plerocercoids from burbot indicate a more dynamic cytoskeleton, increased cell proliferation and differentiation processes, and possibly more mature larvae in the former species. A higher level of expression of the key enzyme of glycolysis and signal transmission in plerocercoids from perch compared to plerocercoids from burbot indicates a greater metabolic activity in larvae from perch. The obtained results indicate that the expression of some proteins in T. nodulosus larvae is influenced by the habitat.