Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom
The prominent myotoxic effects induced by Bothrops jararacussu crude venom are due, in part, to its polycationic myotoxins, BthTX-I and BthTX-II. Both myotoxins have a phospholipase A2 structure: BthTX-II is an active enzyme Asp-49 PLA2, while BthTX-I is a Lys-49 PLA2 devoid of enzymatic activity. I...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:9eefbac9fa6946ddbe93fe8e56b92fb4 2023-05-15T15:08:26+02:00 Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom AM Barbosa AB Villaverde L Guimarães-Sousa AM Soares SF Zamuner JC Cogo SR Zamuner 2010-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992010000300014 https://doaj.org/article/9eefbac9fa6946ddbe93fe8e56b92fb4 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300014 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000300014 1678-9199 https://doaj.org/article/9eefbac9fa6946ddbe93fe8e56b92fb4 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 3, Pp 470-479 (2010) Bothrops jararacussu myotoxins inflammation myonecrosis low-level laser therapy Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2010 ftdoajarticles https://doi.org/10.1590/S1678-91992010000300014 2022-12-31T14:28:51Z The prominent myotoxic effects induced by Bothrops jararacussu crude venom are due, in part, to its polycationic myotoxins, BthTX-I and BthTX-II. Both myotoxins have a phospholipase A2 structure: BthTX-II is an active enzyme Asp-49 PLA2, while BthTX-I is a Lys-49 PLA2 devoid of enzymatic activity. In this study, the effect of low-level laser therapy (LLLT), 685 nm laser at a dose of 4.2 J/cm2 on edema formation, leukocyte influx and myonecrosis caused by BthTX-I and BthTX-II, isolated from Bothrops jararacussu snake venom, was analyzed. BthTX-I and BthTX-II caused a significant edema formation, a prominent leukocyte infiltrate composed predominantly by neutrophils and myonecrosis in envenomed gastrocnemius muscle. LLLT significantly reduced the edema formation, neutrophil accumulation and myonecrosis induced by both myotoxins 24 hours after the injection. LLLT reduced the myonecrosis caused by BthTX-I and BthTX-II, respectively, by 60 and 43%; the edema formation, by 41 and 60.7%; and the leukocyte influx, by 57.5 and 51.6%. In conclusion, LLLT significantly reduced the effect of these snake toxins on the inflammatory response and myonecrosis. These results suggest that LLLT should be considered a potential therapeutic approach for treatment of local effects of Bothrops species venom. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 16 3 470 479 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Bothrops jararacussu myotoxins inflammation myonecrosis low-level laser therapy Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Bothrops jararacussu myotoxins inflammation myonecrosis low-level laser therapy Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 AM Barbosa AB Villaverde L Guimarães-Sousa AM Soares SF Zamuner JC Cogo SR Zamuner Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom |
topic_facet |
Bothrops jararacussu myotoxins inflammation myonecrosis low-level laser therapy Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
The prominent myotoxic effects induced by Bothrops jararacussu crude venom are due, in part, to its polycationic myotoxins, BthTX-I and BthTX-II. Both myotoxins have a phospholipase A2 structure: BthTX-II is an active enzyme Asp-49 PLA2, while BthTX-I is a Lys-49 PLA2 devoid of enzymatic activity. In this study, the effect of low-level laser therapy (LLLT), 685 nm laser at a dose of 4.2 J/cm2 on edema formation, leukocyte influx and myonecrosis caused by BthTX-I and BthTX-II, isolated from Bothrops jararacussu snake venom, was analyzed. BthTX-I and BthTX-II caused a significant edema formation, a prominent leukocyte infiltrate composed predominantly by neutrophils and myonecrosis in envenomed gastrocnemius muscle. LLLT significantly reduced the edema formation, neutrophil accumulation and myonecrosis induced by both myotoxins 24 hours after the injection. LLLT reduced the myonecrosis caused by BthTX-I and BthTX-II, respectively, by 60 and 43%; the edema formation, by 41 and 60.7%; and the leukocyte influx, by 57.5 and 51.6%. In conclusion, LLLT significantly reduced the effect of these snake toxins on the inflammatory response and myonecrosis. These results suggest that LLLT should be considered a potential therapeutic approach for treatment of local effects of Bothrops species venom. |
format |
Article in Journal/Newspaper |
author |
AM Barbosa AB Villaverde L Guimarães-Sousa AM Soares SF Zamuner JC Cogo SR Zamuner |
author_facet |
AM Barbosa AB Villaverde L Guimarães-Sousa AM Soares SF Zamuner JC Cogo SR Zamuner |
author_sort |
AM Barbosa |
title |
Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom |
title_short |
Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom |
title_full |
Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom |
title_fullStr |
Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom |
title_full_unstemmed |
Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom |
title_sort |
low-level laser therapy decreases local effects induced by myotoxins isolated from bothrops jararacussu snake venom |
publisher |
SciELO |
publishDate |
2010 |
url |
https://doi.org/10.1590/S1678-91992010000300014 https://doaj.org/article/9eefbac9fa6946ddbe93fe8e56b92fb4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 3, Pp 470-479 (2010) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300014 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000300014 1678-9199 https://doaj.org/article/9eefbac9fa6946ddbe93fe8e56b92fb4 |
op_doi |
https://doi.org/10.1590/S1678-91992010000300014 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
16 |
container_issue |
3 |
container_start_page |
470 |
op_container_end_page |
479 |
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1766339791813607424 |