The polymorphism and geographical distribution of knockdown resistance of adult Anopheles sinensis populations in eastern China
Abstract Background Anopheles sinensis is one of the major malaria vectors in China and other southeast Asian countries, including Vietnam, Cambodia, Thailand. Vector control is considered to be the critical measure for malaria control, while the increasing prevalence of insecticide resistance cause...
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ftdoajarticles:oai:doaj.org/article:9eb4342a013b42f0a5e30720d0039739 2023-05-15T15:13:41+02:00 The polymorphism and geographical distribution of knockdown resistance of adult Anopheles sinensis populations in eastern China Wei-Long Tan Chun-Xiao Li Rui-Chen Lv Yan-De Dong Xiao-Xia Guo Dan Xing Ming-hao Zhou Yan Xu Hong-liang Chu Gang Wang Chang-qiang Zhu Jun Sun Tong-Yan Zhao 2019-05-01T00:00:00Z https://doi.org/10.1186/s12936-019-2793-z https://doaj.org/article/9eb4342a013b42f0a5e30720d0039739 EN eng BMC http://link.springer.com/article/10.1186/s12936-019-2793-z https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2793-z 1475-2875 https://doaj.org/article/9eb4342a013b42f0a5e30720d0039739 Malaria Journal, Vol 18, Iss 1, Pp 1-12 (2019) Anopheles sinensis Knockdown resistance kdr mutation Pyrethroids Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2019 ftdoajarticles https://doi.org/10.1186/s12936-019-2793-z 2022-12-31T15:12:15Z Abstract Background Anopheles sinensis is one of the major malaria vectors in China and other southeast Asian countries, including Vietnam, Cambodia, Thailand. Vector control is considered to be the critical measure for malaria control, while the increasing prevalence of insecticide resistance caused by long-term use of insecticides, especially pyrethroids, is threatening the successful control of An. sinensis. In order to understand the underlying resistance mechanisms involved and molecular basis, the principal malaria vector, An. sinensis from Jiangsu and Anhui provinces, Southeast China, was investigated. Methods The adult Anopheles mosquitoes were sampled from multiple sites across Jiangsu and Anhui provinces, and sufficient mosquitoes collected from eleven sites for insecticide susceptibility bioassays. The DIIS4–DIIS6 region of the para-type sodium channel gene was amplified and sequenced, then multiple PCR and Taqman assays were used to assess the frequencies of kdr mutations at the target gene. Results In the present study, most of the adult An. sinensis populations were pyrethroids resistant, which indicated the presence of kdr resistance mutations in the para-type sodium channel gene. Sequence analyses demonstrated the kdr mutation existed at codon 1014 in Jiangsu and Anhui provinces. In adult An. sinensis, three mutant types (TTT L1014F, TTC L1014F, and TGT L1014C) of kdr alleles were detected, while no wild type (TTG L1014) was observed. The TTC L1014F mutation was first reported in Anhui province. Conclusions The highly polymorphic kdr alleles were observed in all the adult An. sinensis populations, which suggested that in-depth studies are required for carrying on insecticide resistance monitoring and specific resistance mechanisms studying into establish effective long-term malaria vector control program in eastern China. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 18 1 |
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Anopheles sinensis Knockdown resistance kdr mutation Pyrethroids Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Anopheles sinensis Knockdown resistance kdr mutation Pyrethroids Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Wei-Long Tan Chun-Xiao Li Rui-Chen Lv Yan-De Dong Xiao-Xia Guo Dan Xing Ming-hao Zhou Yan Xu Hong-liang Chu Gang Wang Chang-qiang Zhu Jun Sun Tong-Yan Zhao The polymorphism and geographical distribution of knockdown resistance of adult Anopheles sinensis populations in eastern China |
topic_facet |
Anopheles sinensis Knockdown resistance kdr mutation Pyrethroids Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Anopheles sinensis is one of the major malaria vectors in China and other southeast Asian countries, including Vietnam, Cambodia, Thailand. Vector control is considered to be the critical measure for malaria control, while the increasing prevalence of insecticide resistance caused by long-term use of insecticides, especially pyrethroids, is threatening the successful control of An. sinensis. In order to understand the underlying resistance mechanisms involved and molecular basis, the principal malaria vector, An. sinensis from Jiangsu and Anhui provinces, Southeast China, was investigated. Methods The adult Anopheles mosquitoes were sampled from multiple sites across Jiangsu and Anhui provinces, and sufficient mosquitoes collected from eleven sites for insecticide susceptibility bioassays. The DIIS4–DIIS6 region of the para-type sodium channel gene was amplified and sequenced, then multiple PCR and Taqman assays were used to assess the frequencies of kdr mutations at the target gene. Results In the present study, most of the adult An. sinensis populations were pyrethroids resistant, which indicated the presence of kdr resistance mutations in the para-type sodium channel gene. Sequence analyses demonstrated the kdr mutation existed at codon 1014 in Jiangsu and Anhui provinces. In adult An. sinensis, three mutant types (TTT L1014F, TTC L1014F, and TGT L1014C) of kdr alleles were detected, while no wild type (TTG L1014) was observed. The TTC L1014F mutation was first reported in Anhui province. Conclusions The highly polymorphic kdr alleles were observed in all the adult An. sinensis populations, which suggested that in-depth studies are required for carrying on insecticide resistance monitoring and specific resistance mechanisms studying into establish effective long-term malaria vector control program in eastern China. |
format |
Article in Journal/Newspaper |
author |
Wei-Long Tan Chun-Xiao Li Rui-Chen Lv Yan-De Dong Xiao-Xia Guo Dan Xing Ming-hao Zhou Yan Xu Hong-liang Chu Gang Wang Chang-qiang Zhu Jun Sun Tong-Yan Zhao |
author_facet |
Wei-Long Tan Chun-Xiao Li Rui-Chen Lv Yan-De Dong Xiao-Xia Guo Dan Xing Ming-hao Zhou Yan Xu Hong-liang Chu Gang Wang Chang-qiang Zhu Jun Sun Tong-Yan Zhao |
author_sort |
Wei-Long Tan |
title |
The polymorphism and geographical distribution of knockdown resistance of adult Anopheles sinensis populations in eastern China |
title_short |
The polymorphism and geographical distribution of knockdown resistance of adult Anopheles sinensis populations in eastern China |
title_full |
The polymorphism and geographical distribution of knockdown resistance of adult Anopheles sinensis populations in eastern China |
title_fullStr |
The polymorphism and geographical distribution of knockdown resistance of adult Anopheles sinensis populations in eastern China |
title_full_unstemmed |
The polymorphism and geographical distribution of knockdown resistance of adult Anopheles sinensis populations in eastern China |
title_sort |
polymorphism and geographical distribution of knockdown resistance of adult anopheles sinensis populations in eastern china |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12936-019-2793-z https://doaj.org/article/9eb4342a013b42f0a5e30720d0039739 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 18, Iss 1, Pp 1-12 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s12936-019-2793-z https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2793-z 1475-2875 https://doaj.org/article/9eb4342a013b42f0a5e30720d0039739 |
op_doi |
https://doi.org/10.1186/s12936-019-2793-z |
container_title |
Malaria Journal |
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18 |
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1 |
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1766344212322713600 |