Toxoplasmosis serology: an efficient hemagglutination procedure to detect IgG and IgM antibodies
In search of an efficient but simple, low cost procedure for the serodiagnosis of Toxoplasmosis, especially suited for routine laboratories facing technical and budget limitations as in less developed countries, the diagnostic capability of Hematoxo® , an hemagglutination test for toxoplasmosis, was...
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Universidade de São Paulo (USP)
1989
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ftdoajarticles:oai:doaj.org/article:9eafb01583d44abc928339b50bc4499f 2024-09-09T19:26:52+00:00 Toxoplasmosis serology: an efficient hemagglutination procedure to detect IgG and IgM antibodies M.E. Camargo Maria Emilia G. Moura P.G. Leser 1989-08-01T00:00:00Z https://doi.org/10.1590/S0036-46651989000400012 https://doaj.org/article/9eafb01583d44abc928339b50bc4499f EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651989000400012&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46651989000400012 https://doaj.org/article/9eafb01583d44abc928339b50bc4499f Revista do Instituto de Medicina Tropical de São Paulo, Vol 31, Iss 4, Pp 279-285 (1989) Toxoplasmosis Hemagglutination Serodiagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1989 ftdoajarticles https://doi.org/10.1590/S0036-46651989000400012 2024-08-05T17:49:31Z In search of an efficient but simple, low cost procedure for the serodiagnosis of Toxoplasmosis, especially suited for routine laboratories facing technical and budget limitations as in less developed countries, the diagnostic capability of Hematoxo® , an hemagglutination test for toxoplasmosis, was evaluated in relation to a battery of tests including IgG- and IgM-immunofluorescence tests, hemagglutination and an IgM-capture enzymatic assay. Detecting a little as 5 I.U. of IgG antitoxoplasma antibodies, Hematoxo® showed a straight agreement as to reactivity and non-reactivity for the 443 non-reactive and the 387 reactive serum samples, included in this study. In 23 cases presenting a serological pattern of acute toxoplasmosis and showing IgM antibodies, Hematoxo® could detect IgM antibodies in 18, indicated by negativation or a significant decrease in titers as a result of treating samples with 2-mercapto-ethanol. However, a neat increase in sensitivity for IgM specific antibodies could be achieved by previously removing IgG from the sample, as demonstrated in a series of acute toxoplasmosis sera. A simple procedure was developed for this purpose, by reconstituting a lyophilized suspension of Protein A - rich Staphylococcus with the lowest serum dilution to be tested. Of low cost and easy to perform, Hematoxo® affords not only a practical qualitative procedure for screening reactors and non-reactors, as in prenatal services, but also quantitative assays that permit to titrate antibodies as well as to identify IgM antibodies. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 31 4 279 285 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Toxoplasmosis Hemagglutination Serodiagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Toxoplasmosis Hemagglutination Serodiagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 M.E. Camargo Maria Emilia G. Moura P.G. Leser Toxoplasmosis serology: an efficient hemagglutination procedure to detect IgG and IgM antibodies |
topic_facet |
Toxoplasmosis Hemagglutination Serodiagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
In search of an efficient but simple, low cost procedure for the serodiagnosis of Toxoplasmosis, especially suited for routine laboratories facing technical and budget limitations as in less developed countries, the diagnostic capability of Hematoxo® , an hemagglutination test for toxoplasmosis, was evaluated in relation to a battery of tests including IgG- and IgM-immunofluorescence tests, hemagglutination and an IgM-capture enzymatic assay. Detecting a little as 5 I.U. of IgG antitoxoplasma antibodies, Hematoxo® showed a straight agreement as to reactivity and non-reactivity for the 443 non-reactive and the 387 reactive serum samples, included in this study. In 23 cases presenting a serological pattern of acute toxoplasmosis and showing IgM antibodies, Hematoxo® could detect IgM antibodies in 18, indicated by negativation or a significant decrease in titers as a result of treating samples with 2-mercapto-ethanol. However, a neat increase in sensitivity for IgM specific antibodies could be achieved by previously removing IgG from the sample, as demonstrated in a series of acute toxoplasmosis sera. A simple procedure was developed for this purpose, by reconstituting a lyophilized suspension of Protein A - rich Staphylococcus with the lowest serum dilution to be tested. Of low cost and easy to perform, Hematoxo® affords not only a practical qualitative procedure for screening reactors and non-reactors, as in prenatal services, but also quantitative assays that permit to titrate antibodies as well as to identify IgM antibodies. |
format |
Article in Journal/Newspaper |
author |
M.E. Camargo Maria Emilia G. Moura P.G. Leser |
author_facet |
M.E. Camargo Maria Emilia G. Moura P.G. Leser |
author_sort |
M.E. Camargo |
title |
Toxoplasmosis serology: an efficient hemagglutination procedure to detect IgG and IgM antibodies |
title_short |
Toxoplasmosis serology: an efficient hemagglutination procedure to detect IgG and IgM antibodies |
title_full |
Toxoplasmosis serology: an efficient hemagglutination procedure to detect IgG and IgM antibodies |
title_fullStr |
Toxoplasmosis serology: an efficient hemagglutination procedure to detect IgG and IgM antibodies |
title_full_unstemmed |
Toxoplasmosis serology: an efficient hemagglutination procedure to detect IgG and IgM antibodies |
title_sort |
toxoplasmosis serology: an efficient hemagglutination procedure to detect igg and igm antibodies |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1989 |
url |
https://doi.org/10.1590/S0036-46651989000400012 https://doaj.org/article/9eafb01583d44abc928339b50bc4499f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 31, Iss 4, Pp 279-285 (1989) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651989000400012&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46651989000400012 https://doaj.org/article/9eafb01583d44abc928339b50bc4499f |
op_doi |
https://doi.org/10.1590/S0036-46651989000400012 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
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31 |
container_issue |
4 |
container_start_page |
279 |
op_container_end_page |
285 |
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