Prevalence and the evaluation of culture, wet mount, and ELISA methods for the diagnosis of Trichomonas vaginalis infection among Ghanaian women using urine and vaginal specimens
Abstract Background The services of most clinical laboratories in Africa regarding the diagnosis of Trichomonas vaginalis are largely dependent on the urine direct wet-mount method. However, the exclusive use of urine-based detection may not be appropriate. The culture method is considered the “gold...
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ftdoajarticles:oai:doaj.org/article:9e329f5374fe444ab0de9fe1612b9a22 2023-05-15T15:18:28+02:00 Prevalence and the evaluation of culture, wet mount, and ELISA methods for the diagnosis of Trichomonas vaginalis infection among Ghanaian women using urine and vaginal specimens Collins Adjei Richard Boateng Albert Dompreh Bismark Okyere Eddie-Williams Owiredu 2019-05-01T00:00:00Z https://doi.org/10.1186/s41182-019-0162-9 https://doaj.org/article/9e329f5374fe444ab0de9fe1612b9a22 EN eng BMC http://link.springer.com/article/10.1186/s41182-019-0162-9 https://doaj.org/toc/1349-4147 doi:10.1186/s41182-019-0162-9 1349-4147 https://doaj.org/article/9e329f5374fe444ab0de9fe1612b9a22 Tropical Medicine and Health, Vol 47, Iss 1, Pp 1-8 (2019) Trichomonas vaginalis Culture Wet mount ELISA Arctic medicine. Tropical medicine RC955-962 article 2019 ftdoajarticles https://doi.org/10.1186/s41182-019-0162-9 2022-12-31T03:41:36Z Abstract Background The services of most clinical laboratories in Africa regarding the diagnosis of Trichomonas vaginalis are largely dependent on the urine direct wet-mount method. However, the exclusive use of urine-based detection may not be appropriate. The culture method is considered the “gold standard” for the diagnosis of T. vaginalis. However, this method has a relatively longer turn-around time and is limited by non-viable organisms in the specimen. This study assessed the prevalence of T. vaginalis and its associated risk factors and evaluated its diagnosis using urine and vaginal samples from symptomatic female out-patients by culture, direct wet-mount, and ELISA method respectively. Methods This cross-sectional study was conducted at the Obstetrics and Gynaecology department of the Manhyia District hospital (MDH) and Komfo Anokye Teaching Hospital (KATH), Ghana. Ghanaian sexually active female adults between the ages of 18 and 50 years old were recruited for this study. Vaginal (HVS) and urine samples were collected from each participant, and T. vaginalis infection was assessed based on culture, direct wet mount, and ELISA methods. Results The prevalence of T. vaginalis infection based on the ELISA method, HVS culture, and HVS wet mount were 7.2%, 5.0%, and 1.7%, respectively. Urine culture presented with a 0.6% prevalence rate while urine direct wet mount detected no positive case. There was no statistically significant association between demographic and clinical characteristics and T. vaginalis infection, except for subjects presenting with abdominal pain [OR = 5.42, 95% CI (1.35–21.73), p = 0.017]. Using HVS culture as the reference, ELISA performed best compared to the other methods assessed in this study, presenting with the highest sensitivity [88.9%, 95% CI (54.0–99.8)], specificity [97.1%, 95% CI (93.1–98.9)], AUC (93.0%), and accuracy (96.7%). Conclusion The prevalence of T. vaginalis infection is high among women in Ghana. With the exception of abdominal pain, there is no significant ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Tropical Medicine and Health 47 1 |
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Trichomonas vaginalis Culture Wet mount ELISA Arctic medicine. Tropical medicine RC955-962 |
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Trichomonas vaginalis Culture Wet mount ELISA Arctic medicine. Tropical medicine RC955-962 Collins Adjei Richard Boateng Albert Dompreh Bismark Okyere Eddie-Williams Owiredu Prevalence and the evaluation of culture, wet mount, and ELISA methods for the diagnosis of Trichomonas vaginalis infection among Ghanaian women using urine and vaginal specimens |
topic_facet |
Trichomonas vaginalis Culture Wet mount ELISA Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract Background The services of most clinical laboratories in Africa regarding the diagnosis of Trichomonas vaginalis are largely dependent on the urine direct wet-mount method. However, the exclusive use of urine-based detection may not be appropriate. The culture method is considered the “gold standard” for the diagnosis of T. vaginalis. However, this method has a relatively longer turn-around time and is limited by non-viable organisms in the specimen. This study assessed the prevalence of T. vaginalis and its associated risk factors and evaluated its diagnosis using urine and vaginal samples from symptomatic female out-patients by culture, direct wet-mount, and ELISA method respectively. Methods This cross-sectional study was conducted at the Obstetrics and Gynaecology department of the Manhyia District hospital (MDH) and Komfo Anokye Teaching Hospital (KATH), Ghana. Ghanaian sexually active female adults between the ages of 18 and 50 years old were recruited for this study. Vaginal (HVS) and urine samples were collected from each participant, and T. vaginalis infection was assessed based on culture, direct wet mount, and ELISA methods. Results The prevalence of T. vaginalis infection based on the ELISA method, HVS culture, and HVS wet mount were 7.2%, 5.0%, and 1.7%, respectively. Urine culture presented with a 0.6% prevalence rate while urine direct wet mount detected no positive case. There was no statistically significant association between demographic and clinical characteristics and T. vaginalis infection, except for subjects presenting with abdominal pain [OR = 5.42, 95% CI (1.35–21.73), p = 0.017]. Using HVS culture as the reference, ELISA performed best compared to the other methods assessed in this study, presenting with the highest sensitivity [88.9%, 95% CI (54.0–99.8)], specificity [97.1%, 95% CI (93.1–98.9)], AUC (93.0%), and accuracy (96.7%). Conclusion The prevalence of T. vaginalis infection is high among women in Ghana. With the exception of abdominal pain, there is no significant ... |
format |
Article in Journal/Newspaper |
author |
Collins Adjei Richard Boateng Albert Dompreh Bismark Okyere Eddie-Williams Owiredu |
author_facet |
Collins Adjei Richard Boateng Albert Dompreh Bismark Okyere Eddie-Williams Owiredu |
author_sort |
Collins Adjei |
title |
Prevalence and the evaluation of culture, wet mount, and ELISA methods for the diagnosis of Trichomonas vaginalis infection among Ghanaian women using urine and vaginal specimens |
title_short |
Prevalence and the evaluation of culture, wet mount, and ELISA methods for the diagnosis of Trichomonas vaginalis infection among Ghanaian women using urine and vaginal specimens |
title_full |
Prevalence and the evaluation of culture, wet mount, and ELISA methods for the diagnosis of Trichomonas vaginalis infection among Ghanaian women using urine and vaginal specimens |
title_fullStr |
Prevalence and the evaluation of culture, wet mount, and ELISA methods for the diagnosis of Trichomonas vaginalis infection among Ghanaian women using urine and vaginal specimens |
title_full_unstemmed |
Prevalence and the evaluation of culture, wet mount, and ELISA methods for the diagnosis of Trichomonas vaginalis infection among Ghanaian women using urine and vaginal specimens |
title_sort |
prevalence and the evaluation of culture, wet mount, and elisa methods for the diagnosis of trichomonas vaginalis infection among ghanaian women using urine and vaginal specimens |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s41182-019-0162-9 https://doaj.org/article/9e329f5374fe444ab0de9fe1612b9a22 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Tropical Medicine and Health, Vol 47, Iss 1, Pp 1-8 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s41182-019-0162-9 https://doaj.org/toc/1349-4147 doi:10.1186/s41182-019-0162-9 1349-4147 https://doaj.org/article/9e329f5374fe444ab0de9fe1612b9a22 |
op_doi |
https://doi.org/10.1186/s41182-019-0162-9 |
container_title |
Tropical Medicine and Health |
container_volume |
47 |
container_issue |
1 |
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1766348663637934080 |