Saliva collection and detection of anti- T. gondii antibodies of low-income school-age children as a learning strategy on hygiene, prevention and transmission of toxoplasmosis
ABSTRACT School-age children are a social group in which blood collection for laboratory testing can be perceived as an invasive procedure, with low acceptance and tolerance of stakeholders. This problem could be circumvented by replacing serum samples with saliva. For this purpose, and to make the...
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ftdoajarticles:oai:doaj.org/article:9df4ce8fea3a406594fff3ee28816baf 2024-09-09T19:28:27+00:00 Saliva collection and detection of anti- T. gondii antibodies of low-income school-age children as a learning strategy on hygiene, prevention and transmission of toxoplasmosis Miriam de Souza Macre Luciana Regina Meireles Barbara Fialho Carvalho Sampaio Heitor Franco de Andrade Júnior https://doi.org/10.1590/s1678-9946201961048 https://doaj.org/article/9df4ce8fea3a406594fff3ee28816baf EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652019005000608&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201961048 https://doaj.org/article/9df4ce8fea3a406594fff3ee28816baf Revista do Instituto de Medicina Tropical de São Paulo, Vol 61 Toxoplasma gondii Children Memory Saliva IgG Learning Toxoplasmosis School-age Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article ftdoajarticles https://doi.org/10.1590/s1678-9946201961048 2024-08-05T17:49:30Z ABSTRACT School-age children are a social group in which blood collection for laboratory testing can be perceived as an invasive procedure, with low acceptance and tolerance of stakeholders. This problem could be circumvented by replacing serum samples with saliva. For this purpose, and to make the collection of saliva samples playful and instructive for children, educational activities on hygiene and toxoplasmosis transmission and prevention were performed using toys and audiovisual tools. The target audience consisted of 7-10 year-old children from low-income families who attended public schools in the city of São Paulo. Saliva samples were used in a previously described in-house Enzyme-Linked Immunosorbent Assays (ELISA) to detect anti- Toxoplasma gondii IgG antibodies and establish the immunological status of each of the participants. One year later, children’s memory and fixation of concepts regarding hygiene habits, as well as transmission and prevention of toxoplasmosis were tested in the same schools, by means of a questionnaire application, using students who did not participate in the first intervention as controls. The prevalence of positive anti- T. gondii IgG among students was 50% (82/164). One year later, 45 children had more knowledge on toxoplasmosis (28/45 vs 29/147) and they drew the cat’s involvement in the transmission of toxoplasmosis more often than controls (28/45 vs 29/147). Sorted according to the presence of specific IgG in saliva, recovered positive students presented worse memory of the above cited knowledge as did saliva-negative IgG students, but both groups had isolated higher frequency of fixed knowledge than non-intervened students. Our data show that there is a high prevalence of T. gondii infection in school-children from low-income areas; saliva is an alternative to blood for anti- T. gondii IgG detection; and a one-day educational intervention in school-children was effective in promoting knowledge fixation on hygiene and toxoplasmosis transmission and prevention after one ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 61 |
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Toxoplasma gondii Children Memory Saliva IgG Learning Toxoplasmosis School-age Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Toxoplasma gondii Children Memory Saliva IgG Learning Toxoplasmosis School-age Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Miriam de Souza Macre Luciana Regina Meireles Barbara Fialho Carvalho Sampaio Heitor Franco de Andrade Júnior Saliva collection and detection of anti- T. gondii antibodies of low-income school-age children as a learning strategy on hygiene, prevention and transmission of toxoplasmosis |
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Toxoplasma gondii Children Memory Saliva IgG Learning Toxoplasmosis School-age Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT School-age children are a social group in which blood collection for laboratory testing can be perceived as an invasive procedure, with low acceptance and tolerance of stakeholders. This problem could be circumvented by replacing serum samples with saliva. For this purpose, and to make the collection of saliva samples playful and instructive for children, educational activities on hygiene and toxoplasmosis transmission and prevention were performed using toys and audiovisual tools. The target audience consisted of 7-10 year-old children from low-income families who attended public schools in the city of São Paulo. Saliva samples were used in a previously described in-house Enzyme-Linked Immunosorbent Assays (ELISA) to detect anti- Toxoplasma gondii IgG antibodies and establish the immunological status of each of the participants. One year later, children’s memory and fixation of concepts regarding hygiene habits, as well as transmission and prevention of toxoplasmosis were tested in the same schools, by means of a questionnaire application, using students who did not participate in the first intervention as controls. The prevalence of positive anti- T. gondii IgG among students was 50% (82/164). One year later, 45 children had more knowledge on toxoplasmosis (28/45 vs 29/147) and they drew the cat’s involvement in the transmission of toxoplasmosis more often than controls (28/45 vs 29/147). Sorted according to the presence of specific IgG in saliva, recovered positive students presented worse memory of the above cited knowledge as did saliva-negative IgG students, but both groups had isolated higher frequency of fixed knowledge than non-intervened students. Our data show that there is a high prevalence of T. gondii infection in school-children from low-income areas; saliva is an alternative to blood for anti- T. gondii IgG detection; and a one-day educational intervention in school-children was effective in promoting knowledge fixation on hygiene and toxoplasmosis transmission and prevention after one ... |
format |
Article in Journal/Newspaper |
author |
Miriam de Souza Macre Luciana Regina Meireles Barbara Fialho Carvalho Sampaio Heitor Franco de Andrade Júnior |
author_facet |
Miriam de Souza Macre Luciana Regina Meireles Barbara Fialho Carvalho Sampaio Heitor Franco de Andrade Júnior |
author_sort |
Miriam de Souza Macre |
title |
Saliva collection and detection of anti- T. gondii antibodies of low-income school-age children as a learning strategy on hygiene, prevention and transmission of toxoplasmosis |
title_short |
Saliva collection and detection of anti- T. gondii antibodies of low-income school-age children as a learning strategy on hygiene, prevention and transmission of toxoplasmosis |
title_full |
Saliva collection and detection of anti- T. gondii antibodies of low-income school-age children as a learning strategy on hygiene, prevention and transmission of toxoplasmosis |
title_fullStr |
Saliva collection and detection of anti- T. gondii antibodies of low-income school-age children as a learning strategy on hygiene, prevention and transmission of toxoplasmosis |
title_full_unstemmed |
Saliva collection and detection of anti- T. gondii antibodies of low-income school-age children as a learning strategy on hygiene, prevention and transmission of toxoplasmosis |
title_sort |
saliva collection and detection of anti- t. gondii antibodies of low-income school-age children as a learning strategy on hygiene, prevention and transmission of toxoplasmosis |
publisher |
Universidade de São Paulo (USP) |
url |
https://doi.org/10.1590/s1678-9946201961048 https://doaj.org/article/9df4ce8fea3a406594fff3ee28816baf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 61 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652019005000608&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201961048 https://doaj.org/article/9df4ce8fea3a406594fff3ee28816baf |
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https://doi.org/10.1590/s1678-9946201961048 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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61 |
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