Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss
Bryophytes are the dominant vegetation in the Antarctic continent. They have suffered more unpleasant ultraviolet radiation due to the Antarctic ozone layer destruction. However, it remains unclear about the molecular mechanism of Antarctic moss acclimation to UV-B light. Here, the transcriptomics a...
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ftdoajarticles:oai:doaj.org/article:9dd413fed743441aa9660e4d60efe453 2023-05-15T14:05:04+02:00 Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss Shenghao Liu Shuo Fang Chenlin Liu Linlin Zhao Bailin Cong Zhaohui Zhang 2021-12-01T00:00:00Z https://doi.org/10.3389/fpls.2021.788377 https://doaj.org/article/9dd413fed743441aa9660e4d60efe453 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fpls.2021.788377/full https://doaj.org/toc/1664-462X 1664-462X doi:10.3389/fpls.2021.788377 https://doaj.org/article/9dd413fed743441aa9660e4d60efe453 Frontiers in Plant Science, Vol 12 (2021) abiotic stress bryophytes flavonoids metabolome transcriptome ultraviolet-B radiation Plant culture SB1-1110 article 2021 ftdoajarticles https://doi.org/10.3389/fpls.2021.788377 2022-12-31T05:20:26Z Bryophytes are the dominant vegetation in the Antarctic continent. They have suffered more unpleasant ultraviolet radiation due to the Antarctic ozone layer destruction. However, it remains unclear about the molecular mechanism of Antarctic moss acclimation to UV-B light. Here, the transcriptomics and metabolomics approaches were conducted to uncover transcriptional and metabolic profiling of the Antarctic moss Leptobryum pyriforme under UV-B radiation. Totally, 67,290 unigenes with N50 length of 2,055 bp were assembled. Of them, 1,594 unigenes were significantly up-regulated and 3353 unigenes were markedly down-regulated under UV-B radiation. These differentially expressed genes (DEGs) involved in UV-B signaling, flavonoid biosynthesis, ROS scavenging, and DNA repair. In addition, a total of 531 metabolites were detected, while flavonoids and anthocyanins accounted for 10.36% of the total compounds. There were 49 upregulated metabolites and 41 downregulated metabolites under UV-B radiation. Flavonoids were the most significantly changed metabolites. qPCR analysis showed that UVR8-COP1-HY5 signaling pathway genes and photolyase genes (i.e., LpUVR3, LpPHR1, and LpDPL) were significantly up-regulated under UV-B light. In addition, the expression levels of JA signaling pathway-related genes (i.e., OPR and JAZ) and flavonoid biosynthesis-related genes were also significantly increased under UV-B radiation. The integrative data analysis showed that UVR8-mediated signaling, jasmonate signaling, flavonoid biosynthesis pathway and DNA repair system might contribute to L. pyriforme acclimating to UV-B radiation. Therefore, these findings present a novel knowledge for understanding the adaption of Antarctic moss to polar environments and provide a foundation for assessing the impact of global climate change on Antarctic land plants. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Frontiers in Plant Science 12 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
abiotic stress bryophytes flavonoids metabolome transcriptome ultraviolet-B radiation Plant culture SB1-1110 |
spellingShingle |
abiotic stress bryophytes flavonoids metabolome transcriptome ultraviolet-B radiation Plant culture SB1-1110 Shenghao Liu Shuo Fang Chenlin Liu Linlin Zhao Bailin Cong Zhaohui Zhang Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss |
topic_facet |
abiotic stress bryophytes flavonoids metabolome transcriptome ultraviolet-B radiation Plant culture SB1-1110 |
description |
Bryophytes are the dominant vegetation in the Antarctic continent. They have suffered more unpleasant ultraviolet radiation due to the Antarctic ozone layer destruction. However, it remains unclear about the molecular mechanism of Antarctic moss acclimation to UV-B light. Here, the transcriptomics and metabolomics approaches were conducted to uncover transcriptional and metabolic profiling of the Antarctic moss Leptobryum pyriforme under UV-B radiation. Totally, 67,290 unigenes with N50 length of 2,055 bp were assembled. Of them, 1,594 unigenes were significantly up-regulated and 3353 unigenes were markedly down-regulated under UV-B radiation. These differentially expressed genes (DEGs) involved in UV-B signaling, flavonoid biosynthesis, ROS scavenging, and DNA repair. In addition, a total of 531 metabolites were detected, while flavonoids and anthocyanins accounted for 10.36% of the total compounds. There were 49 upregulated metabolites and 41 downregulated metabolites under UV-B radiation. Flavonoids were the most significantly changed metabolites. qPCR analysis showed that UVR8-COP1-HY5 signaling pathway genes and photolyase genes (i.e., LpUVR3, LpPHR1, and LpDPL) were significantly up-regulated under UV-B light. In addition, the expression levels of JA signaling pathway-related genes (i.e., OPR and JAZ) and flavonoid biosynthesis-related genes were also significantly increased under UV-B radiation. The integrative data analysis showed that UVR8-mediated signaling, jasmonate signaling, flavonoid biosynthesis pathway and DNA repair system might contribute to L. pyriforme acclimating to UV-B radiation. Therefore, these findings present a novel knowledge for understanding the adaption of Antarctic moss to polar environments and provide a foundation for assessing the impact of global climate change on Antarctic land plants. |
format |
Article in Journal/Newspaper |
author |
Shenghao Liu Shuo Fang Chenlin Liu Linlin Zhao Bailin Cong Zhaohui Zhang |
author_facet |
Shenghao Liu Shuo Fang Chenlin Liu Linlin Zhao Bailin Cong Zhaohui Zhang |
author_sort |
Shenghao Liu |
title |
Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss |
title_short |
Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss |
title_full |
Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss |
title_fullStr |
Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss |
title_full_unstemmed |
Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss |
title_sort |
transcriptomics integrated with metabolomics reveal the effects of ultraviolet-b radiation on flavonoid biosynthesis in antarctic moss |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doi.org/10.3389/fpls.2021.788377 https://doaj.org/article/9dd413fed743441aa9660e4d60efe453 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Frontiers in Plant Science, Vol 12 (2021) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fpls.2021.788377/full https://doaj.org/toc/1664-462X 1664-462X doi:10.3389/fpls.2021.788377 https://doaj.org/article/9dd413fed743441aa9660e4d60efe453 |
op_doi |
https://doi.org/10.3389/fpls.2021.788377 |
container_title |
Frontiers in Plant Science |
container_volume |
12 |
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1766276711626833920 |