Comparison of direct immunofluorescence, conventional cell culture and polymerase chain reaction techniques for detecting respiratory syncytial virus in nasopharyngeal aspirates from infants Comparação entre imunofluorescência direta, cultura convencional de células e reação em cadeia de polimerase para detectar vírus respiratório sincicial em lavados de nasofaringe de crianças

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: Alexanda Dias Reis, Maria Cristina Domingues Fink, Clarisse Martins Machado, José de Paula Paz Jr., Renato Reis Oliveira, Adriana Fumie Tateno, Adriana Freire Machado, Maria Regina Cardoso, Claudio Sérgio Pannuti
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 2008
• Subjects: Respiratory syncytial virus; Infants; Diagnosis; Cell culture; Polymerase chain reaction; Immunofluorescence; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Aguda; Arctic
• Online Access: https://doi.org/10.1590/S0036-46652008000100008; https://doaj.org/article/9dc1b14db5d5479f842a5c7c02a28023

A total of 316 samples of nasopharyngeal aspirate from infants up to two years of age with acute respiratory-tract illnesses were processed for detection of respiratory syncytial virus (RSV) using three different techniques: viral isolation, direct immunofluorescence, and PCR. Of the samples, 36 (11...