Prevalence and virulence factors of Candida spp. associated with blow flies
Objective: To investigate the prevalence of Candida spp. and the virulence factors of Candida albicans (C. albicans) isolated from external surfaces of blow flies collected from Mae Sot, Tak Province, Thailand. Methods: The blow flies were collected by sterile sweep nets from three areas in Mae Sot....
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:9db36eefdf8946e68c514474479e44d4 2023-05-15T15:09:59+02:00 Prevalence and virulence factors of Candida spp. associated with blow flies Wimonrat GunTang Nathamon Kamonvoradej Chitchanok Chomchat Sangrawee Suriyakan Sangob Sanit Jintana Wongwigkarn Nophawan Bunchu Damrongpan Thongwat Supaporn Lamlertthon 2017-05-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2017.01.014 https://doaj.org/article/9db36eefdf8946e68c514474479e44d4 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169116304324 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.01.014 https://doaj.org/article/9db36eefdf8946e68c514474479e44d4 Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 5, Pp 428-431 (2017) Blow flies Candida albicans β-Hemolysin Phospholipase Antifungal resistance Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2017.01.014 2022-12-31T14:09:11Z Objective: To investigate the prevalence of Candida spp. and the virulence factors of Candida albicans (C. albicans) isolated from external surfaces of blow flies collected from Mae Sot, Tak Province, Thailand. Methods: The blow flies were collected by sterile sweep nets from three areas in Mae Sot. Yeast isolation was first performed on Sabouraud dextrose agar (SDA) supplemented with chloramphenicol and cycloheximide. The yeast isolates were then identified by using chromogenic agar, a yeast identification test kit, a germ tube formation test and a carbohydrate utilization test. The β-hemolysis was determined on 7% sheep blood agar, while phospholipase activity was measured on SDA agar supplemented with 10% egg yolk suspension. Antifungal susceptibility testing was determined by broth micro-dilution testing against ketoconazole and amphotericin B. Results: The prevalence rate of Candida spp. on the external surfaces of the blow flies was 78.1%. All C. albicans isolated from the blow fly demonstrated β-hemolysin and potent phospholipase activities and 47.1% of C. albicans were resistant to ketoconazole with MIC values 128 μg/mL. Conclusions: The results indicate that blow flies could play an essential role in the transmission of potentially pathogenic and antifungal resistant C. albicans into the environment. Further investigation on other virulence factors and genetic relatedness among isolates from the blow flies, the environment and clinical specimens is required to confirm this role. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 7 5 428 431 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Blow flies Candida albicans β-Hemolysin Phospholipase Antifungal resistance Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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Blow flies Candida albicans β-Hemolysin Phospholipase Antifungal resistance Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Wimonrat GunTang Nathamon Kamonvoradej Chitchanok Chomchat Sangrawee Suriyakan Sangob Sanit Jintana Wongwigkarn Nophawan Bunchu Damrongpan Thongwat Supaporn Lamlertthon Prevalence and virulence factors of Candida spp. associated with blow flies |
topic_facet |
Blow flies Candida albicans β-Hemolysin Phospholipase Antifungal resistance Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the prevalence of Candida spp. and the virulence factors of Candida albicans (C. albicans) isolated from external surfaces of blow flies collected from Mae Sot, Tak Province, Thailand. Methods: The blow flies were collected by sterile sweep nets from three areas in Mae Sot. Yeast isolation was first performed on Sabouraud dextrose agar (SDA) supplemented with chloramphenicol and cycloheximide. The yeast isolates were then identified by using chromogenic agar, a yeast identification test kit, a germ tube formation test and a carbohydrate utilization test. The β-hemolysis was determined on 7% sheep blood agar, while phospholipase activity was measured on SDA agar supplemented with 10% egg yolk suspension. Antifungal susceptibility testing was determined by broth micro-dilution testing against ketoconazole and amphotericin B. Results: The prevalence rate of Candida spp. on the external surfaces of the blow flies was 78.1%. All C. albicans isolated from the blow fly demonstrated β-hemolysin and potent phospholipase activities and 47.1% of C. albicans were resistant to ketoconazole with MIC values 128 μg/mL. Conclusions: The results indicate that blow flies could play an essential role in the transmission of potentially pathogenic and antifungal resistant C. albicans into the environment. Further investigation on other virulence factors and genetic relatedness among isolates from the blow flies, the environment and clinical specimens is required to confirm this role. |
format |
Article in Journal/Newspaper |
author |
Wimonrat GunTang Nathamon Kamonvoradej Chitchanok Chomchat Sangrawee Suriyakan Sangob Sanit Jintana Wongwigkarn Nophawan Bunchu Damrongpan Thongwat Supaporn Lamlertthon |
author_facet |
Wimonrat GunTang Nathamon Kamonvoradej Chitchanok Chomchat Sangrawee Suriyakan Sangob Sanit Jintana Wongwigkarn Nophawan Bunchu Damrongpan Thongwat Supaporn Lamlertthon |
author_sort |
Wimonrat GunTang |
title |
Prevalence and virulence factors of Candida spp. associated with blow flies |
title_short |
Prevalence and virulence factors of Candida spp. associated with blow flies |
title_full |
Prevalence and virulence factors of Candida spp. associated with blow flies |
title_fullStr |
Prevalence and virulence factors of Candida spp. associated with blow flies |
title_full_unstemmed |
Prevalence and virulence factors of Candida spp. associated with blow flies |
title_sort |
prevalence and virulence factors of candida spp. associated with blow flies |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2017.01.014 https://doaj.org/article/9db36eefdf8946e68c514474479e44d4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 5, Pp 428-431 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169116304324 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.01.014 https://doaj.org/article/9db36eefdf8946e68c514474479e44d4 |
op_doi |
https://doi.org/10.1016/j.apjtb.2017.01.014 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
5 |
container_start_page |
428 |
op_container_end_page |
431 |
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1766341064713568256 |