Venom composition of Trimeresurus albolabris, T. insularis, T. puniceus and T. purpureomaculatus from Indonesia
Abstract Background: Several studies have been published on the characterization of Trimeresurus venoms. However, there is still limited information concerning the venom composition of Trimeresurus species distributed throughout Indonesia, which contributes to significant snakebite envenomation case...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/1678-9199-jvatitd-2021-0103 https://doaj.org/article/9daf548bddbf4176b1fa6c4770a2c18c |
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ftdoajarticles:oai:doaj.org/article:9daf548bddbf4176b1fa6c4770a2c18c 2023-05-15T15:16:21+02:00 Venom composition of Trimeresurus albolabris, T. insularis, T. puniceus and T. purpureomaculatus from Indonesia Syahfitri Anita Arif Rahman Sadjuri Latri Rahmah Herjuno Ari Nugroho Mulyadi Wahyu Trilaksono Wiwit Ridhani Nabila Safira Hariman Bahtiar Maharani Amir Hamidy Adriansjah Azhari 2022-07-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-2021-0103 https://doaj.org/article/9daf548bddbf4176b1fa6c4770a2c18c EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992022000100315&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2021-0103 https://doaj.org/article/9daf548bddbf4176b1fa6c4770a2c18c Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 28 (2022) Trimeresurus albolabris Trimeresurus insularis Trimeresurus puniceus Trimeresurus purpureomaculatus Snake venom Venom proteome Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2022 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2021-0103 2022-12-31T01:13:18Z Abstract Background: Several studies have been published on the characterization of Trimeresurus venoms. However, there is still limited information concerning the venom composition of Trimeresurus species distributed throughout Indonesia, which contributes to significant snakebite envenomation cases. The present study describes a comparative on the composition of T. albolabris, T. insularis, T. puniceus, and T. purpureomaculatus venoms originated from Indonesia. Methods: Protein content in the venom of four Trimeresurus species was determined using Bradford assay, and the venom proteome was elucidated using one-dimension SDS PAGE nano-ESI- LCMS/MS shotgun proteomics. Results: The venom of T. albolabris contained the highest protein content of 11.1 mg/mL, followed by T. puniceus, T. insularis and T. purpureomaculatus venom with 10.7 mg/mL, 8.9 mg/mL and 5.54 mg/mL protein, respectively. In total, our venomic analysis identified 65 proteins belonging to 16 protein families in T. purpureomaculatus; 64 proteins belonging to 18 protein families in T. albolabris; 58 different proteins belonging to 14 protein families in T. puniceus; and 48 different proteins belonging to 14 protein familiesin T. insularis. Four major proteins identified in all venoms belonged to snake venom metalloproteinase, C-type lectin, snake venom serine protease, and phospholipase A2. There were 11 common proteins in all venoms, and T. puniceus venom has the highest number of unique proteins compared to the other three venoms. Cluster analysis of the proteins and venoms showed that T. puniceus venom has the most distinct venom composition. Conclusions: Overall, the results highlighted venom compositional variation of four Trimeresurus spp. from Indonesia. The venoms appear to be highly similar, comprising at least four protein families that correlate with venom’s toxin properties and function. This study adds more information on venom variability among Trimeresurus species within the close geographic origin and may contribute to the development ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 28 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Trimeresurus albolabris Trimeresurus insularis Trimeresurus puniceus Trimeresurus purpureomaculatus Snake venom Venom proteome Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Trimeresurus albolabris Trimeresurus insularis Trimeresurus puniceus Trimeresurus purpureomaculatus Snake venom Venom proteome Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Syahfitri Anita Arif Rahman Sadjuri Latri Rahmah Herjuno Ari Nugroho Mulyadi Wahyu Trilaksono Wiwit Ridhani Nabila Safira Hariman Bahtiar Maharani Amir Hamidy Adriansjah Azhari Venom composition of Trimeresurus albolabris, T. insularis, T. puniceus and T. purpureomaculatus from Indonesia |
topic_facet |
Trimeresurus albolabris Trimeresurus insularis Trimeresurus puniceus Trimeresurus purpureomaculatus Snake venom Venom proteome Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: Several studies have been published on the characterization of Trimeresurus venoms. However, there is still limited information concerning the venom composition of Trimeresurus species distributed throughout Indonesia, which contributes to significant snakebite envenomation cases. The present study describes a comparative on the composition of T. albolabris, T. insularis, T. puniceus, and T. purpureomaculatus venoms originated from Indonesia. Methods: Protein content in the venom of four Trimeresurus species was determined using Bradford assay, and the venom proteome was elucidated using one-dimension SDS PAGE nano-ESI- LCMS/MS shotgun proteomics. Results: The venom of T. albolabris contained the highest protein content of 11.1 mg/mL, followed by T. puniceus, T. insularis and T. purpureomaculatus venom with 10.7 mg/mL, 8.9 mg/mL and 5.54 mg/mL protein, respectively. In total, our venomic analysis identified 65 proteins belonging to 16 protein families in T. purpureomaculatus; 64 proteins belonging to 18 protein families in T. albolabris; 58 different proteins belonging to 14 protein families in T. puniceus; and 48 different proteins belonging to 14 protein familiesin T. insularis. Four major proteins identified in all venoms belonged to snake venom metalloproteinase, C-type lectin, snake venom serine protease, and phospholipase A2. There were 11 common proteins in all venoms, and T. puniceus venom has the highest number of unique proteins compared to the other three venoms. Cluster analysis of the proteins and venoms showed that T. puniceus venom has the most distinct venom composition. Conclusions: Overall, the results highlighted venom compositional variation of four Trimeresurus spp. from Indonesia. The venoms appear to be highly similar, comprising at least four protein families that correlate with venom’s toxin properties and function. This study adds more information on venom variability among Trimeresurus species within the close geographic origin and may contribute to the development ... |
format |
Article in Journal/Newspaper |
author |
Syahfitri Anita Arif Rahman Sadjuri Latri Rahmah Herjuno Ari Nugroho Mulyadi Wahyu Trilaksono Wiwit Ridhani Nabila Safira Hariman Bahtiar Maharani Amir Hamidy Adriansjah Azhari |
author_facet |
Syahfitri Anita Arif Rahman Sadjuri Latri Rahmah Herjuno Ari Nugroho Mulyadi Wahyu Trilaksono Wiwit Ridhani Nabila Safira Hariman Bahtiar Maharani Amir Hamidy Adriansjah Azhari |
author_sort |
Syahfitri Anita |
title |
Venom composition of Trimeresurus albolabris, T. insularis, T. puniceus and T. purpureomaculatus from Indonesia |
title_short |
Venom composition of Trimeresurus albolabris, T. insularis, T. puniceus and T. purpureomaculatus from Indonesia |
title_full |
Venom composition of Trimeresurus albolabris, T. insularis, T. puniceus and T. purpureomaculatus from Indonesia |
title_fullStr |
Venom composition of Trimeresurus albolabris, T. insularis, T. puniceus and T. purpureomaculatus from Indonesia |
title_full_unstemmed |
Venom composition of Trimeresurus albolabris, T. insularis, T. puniceus and T. purpureomaculatus from Indonesia |
title_sort |
venom composition of trimeresurus albolabris, t. insularis, t. puniceus and t. purpureomaculatus from indonesia |
publisher |
SciELO |
publishDate |
2022 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2021-0103 https://doaj.org/article/9daf548bddbf4176b1fa6c4770a2c18c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 28 (2022) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992022000100315&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2021-0103 https://doaj.org/article/9daf548bddbf4176b1fa6c4770a2c18c |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2021-0103 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
28 |
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1766346644719140864 |