Characterization of Molecular Species and Anti-Inflammatory Activity of Purified Phospholipids from Antarctic Krill Oil

• Published in: Marine Drugs
• Main Authors: Li Zhou, Xing Wu, Fu Yang, Minghao Zhang, Rong Huang, Jikai Liu
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2021
• Subjects: Antarctic krill oil; phospholipids; UHPLC-Q-TOF-MS; anti-inflammation; Biology (General); QH301-705.5; Antarctic; The Antarctic; Antarc*; Antarctic Krill
• Online Access: https://doi.org/10.3390/md19030124; https://doaj.org/article/9d7732ec79ae47baa3c229bc05c855ad

The phospholipids (PLs) from Antarctic krill oil were purified (>97.2%) using adsorption column chromatography. Forty-nine PL molecular species were characterized by ultrahigh-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF-MS). Most of molecular species contained eicosapentaenoic acid (EPA, 20:5), docosahexaenoic acid (DHA, 22:6), docosapentaenoic acid (DPA, 22:5), and arachidonic acid (AA, 20:4). Notably, a special species PC (20:5/22:6) (1298.17 nmol/g) and many ether PLs were detected. The Antarctic krill PL liposome (IC 50 = 0.108 mg/mL) showed better anti-inflammatory activity than crude Antarctic krill oil (IC 50 = 0.446 mg/mL). It could block NF-κB signaling pathway via suppression of IκB-α degradation and p65 activation and dose-dependently reduce the cellular content of inflammatory mediators including nitric oxide (NO), reactive oxygen species (ROS), and inflammatory cytokines in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. In addition, it can suppress carrageenan-induced mouse paw swelling. Results from the present study could provide a reference for better evaluation of nutritional and medicinal values of Antarctic krill oil.