The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia ( Oreochromis niloticus ) and Atlantic Cod ( Gadus morhua L.)
Francisellosis in fish is caused by the facultative intracellular Gram-negative bacterial pathogens Francisella noatunensis ssp. noatunensis and Francisella orientalis . The disease is affecting both farmed and wild fish worldwide and no commercial vaccines are currently available. In this study, we...
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ftdoajarticles:oai:doaj.org/article:9d69cb23e77645979008fe1fe7d4866a 2024-01-07T09:42:07+01:00 The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia ( Oreochromis niloticus ) and Atlantic Cod ( Gadus morhua L.) Verena Mertes Alexander Kashulin Bekkelund Leidy Lagos Elia Ciani Duncan Colquhoun Hanne Haslene-Hox Håvard Sletta Henning Sørum Hanne Cecilie Winther-Larsen 2021-01-01T00:00:00Z https://doi.org/10.3390/vaccines9010034 https://doaj.org/article/9d69cb23e77645979008fe1fe7d4866a EN eng MDPI AG https://www.mdpi.com/2076-393X/9/1/34 https://doaj.org/toc/2076-393X doi:10.3390/vaccines9010034 2076-393X https://doaj.org/article/9d69cb23e77645979008fe1fe7d4866a Vaccines, Vol 9, Iss 1, p 34 (2021) francisellosis vaccine membrane vesicles fish disease Atlantic cod tilapia Medicine R article 2021 ftdoajarticles https://doi.org/10.3390/vaccines9010034 2023-12-10T01:47:16Z Francisellosis in fish is caused by the facultative intracellular Gram-negative bacterial pathogens Francisella noatunensis ssp. noatunensis and Francisella orientalis . The disease is affecting both farmed and wild fish worldwide and no commercial vaccines are currently available. In this study, we tested isolated membrane vesicles (MVs) as possible vaccine candidates based on previous trials in zebrafish ( Danio rerio ) indicating promising vaccine efficacy. Here, the MV vaccine-candidates were tested in their natural hosts, Atlantic cod ( Gadus morhua L.) and Nile tilapia ( Oreochromis niloticus ). Injection of MVs did not display any toxicity or other negative influence on the fish and gene expression analysis indicated an influence on the host immune response. However, unlike in other tested fish species, a protective immunity following vaccine application and immunization period could not be detected in the Atlantic cod or tilapia. Further in vivo studies are required to achieve a better understanding of the development of immunological memory in different fish species. Article in Journal/Newspaper atlantic cod Gadus morhua Directory of Open Access Journals: DOAJ Articles Vaccines 9 1 34 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
francisellosis vaccine membrane vesicles fish disease Atlantic cod tilapia Medicine R |
spellingShingle |
francisellosis vaccine membrane vesicles fish disease Atlantic cod tilapia Medicine R Verena Mertes Alexander Kashulin Bekkelund Leidy Lagos Elia Ciani Duncan Colquhoun Hanne Haslene-Hox Håvard Sletta Henning Sørum Hanne Cecilie Winther-Larsen The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia ( Oreochromis niloticus ) and Atlantic Cod ( Gadus morhua L.) |
topic_facet |
francisellosis vaccine membrane vesicles fish disease Atlantic cod tilapia Medicine R |
description |
Francisellosis in fish is caused by the facultative intracellular Gram-negative bacterial pathogens Francisella noatunensis ssp. noatunensis and Francisella orientalis . The disease is affecting both farmed and wild fish worldwide and no commercial vaccines are currently available. In this study, we tested isolated membrane vesicles (MVs) as possible vaccine candidates based on previous trials in zebrafish ( Danio rerio ) indicating promising vaccine efficacy. Here, the MV vaccine-candidates were tested in their natural hosts, Atlantic cod ( Gadus morhua L.) and Nile tilapia ( Oreochromis niloticus ). Injection of MVs did not display any toxicity or other negative influence on the fish and gene expression analysis indicated an influence on the host immune response. However, unlike in other tested fish species, a protective immunity following vaccine application and immunization period could not be detected in the Atlantic cod or tilapia. Further in vivo studies are required to achieve a better understanding of the development of immunological memory in different fish species. |
format |
Article in Journal/Newspaper |
author |
Verena Mertes Alexander Kashulin Bekkelund Leidy Lagos Elia Ciani Duncan Colquhoun Hanne Haslene-Hox Håvard Sletta Henning Sørum Hanne Cecilie Winther-Larsen |
author_facet |
Verena Mertes Alexander Kashulin Bekkelund Leidy Lagos Elia Ciani Duncan Colquhoun Hanne Haslene-Hox Håvard Sletta Henning Sørum Hanne Cecilie Winther-Larsen |
author_sort |
Verena Mertes |
title |
The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia ( Oreochromis niloticus ) and Atlantic Cod ( Gadus morhua L.) |
title_short |
The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia ( Oreochromis niloticus ) and Atlantic Cod ( Gadus morhua L.) |
title_full |
The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia ( Oreochromis niloticus ) and Atlantic Cod ( Gadus morhua L.) |
title_fullStr |
The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia ( Oreochromis niloticus ) and Atlantic Cod ( Gadus morhua L.) |
title_full_unstemmed |
The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia ( Oreochromis niloticus ) and Atlantic Cod ( Gadus morhua L.) |
title_sort |
use of extracellular membrane vesicles for immunization against francisellosis in nile tilapia ( oreochromis niloticus ) and atlantic cod ( gadus morhua l.) |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/vaccines9010034 https://doaj.org/article/9d69cb23e77645979008fe1fe7d4866a |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Vaccines, Vol 9, Iss 1, p 34 (2021) |
op_relation |
https://www.mdpi.com/2076-393X/9/1/34 https://doaj.org/toc/2076-393X doi:10.3390/vaccines9010034 2076-393X https://doaj.org/article/9d69cb23e77645979008fe1fe7d4866a |
op_doi |
https://doi.org/10.3390/vaccines9010034 |
container_title |
Vaccines |
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9 |
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1 |
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34 |
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