Purification and partial characterization of a coagulant serine protease from the venom of the Iranian snake Agkistrodon halys
Agkistrodon halys is one of several dangerous snake species in Iran. Among the most important signs and symptoms in patients envenomated by this snake is disseminated intravascular coagulation. A thrombin-like enzyme, called AH143, was isolated from Agkistrodon halys venom by gel filtration on a Sep...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:9d6995f45b914f26a7052c3dd83c5cd2 2023-05-15T15:08:11+02:00 Purification and partial characterization of a coagulant serine protease from the venom of the Iranian snake Agkistrodon halys M Ghorbanpur A Zare Mirakabadi F Zokaee H Zolfagarrian H Rabiei 2009-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992009000300005 https://doaj.org/article/9d6995f45b914f26a7052c3dd83c5cd2 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300005 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992009000300005 1678-9199 https://doaj.org/article/9d6995f45b914f26a7052c3dd83c5cd2 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 15, Iss 3, Pp 411-423 (2009) Iranian snake venom Agkistrodon halys serine protease chromatography coagulant activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2009 ftdoajarticles https://doi.org/10.1590/S1678-91992009000300005 2022-12-31T02:14:45Z Agkistrodon halys is one of several dangerous snake species in Iran. Among the most important signs and symptoms in patients envenomated by this snake is disseminated intravascular coagulation. A thrombin-like enzyme, called AH143, was isolated from Agkistrodon halys venom by gel filtration on a Sephadex G-50 column, ion-exchange chromatography on a DEAE-Sepharose and high performance liquid chromatography (HPLC) on a C18 column. In the final stage of purification, 0.82 mg of purified enzyme was obtained from 182.5 mg of venom. The purified enzyme showed a single protein band by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), under reducing conditions, and its molecular mass was found to be about 30 kDa. AH143 revealed clotting activity in human plasma, which was not inhibited by EDTA or heparin. This enzyme still demonstrated coagulation activity when exposed to variations in temperature and pH ranging, respectively, from 30 to 40°C and from 7.0 to 8.0. It also displayed proteolytic activities on synthetic substrate. The purified enzyme did not show any effect on casein. We concluded that the venom of the Iranian snake Agkistrodon halys contains about 0.45% single procoagulant protein which appears to be a thrombin-like enzyme. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 15 3 411 423 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Iranian snake venom Agkistrodon halys serine protease chromatography coagulant activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
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Iranian snake venom Agkistrodon halys serine protease chromatography coagulant activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 M Ghorbanpur A Zare Mirakabadi F Zokaee H Zolfagarrian H Rabiei Purification and partial characterization of a coagulant serine protease from the venom of the Iranian snake Agkistrodon halys |
topic_facet |
Iranian snake venom Agkistrodon halys serine protease chromatography coagulant activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Agkistrodon halys is one of several dangerous snake species in Iran. Among the most important signs and symptoms in patients envenomated by this snake is disseminated intravascular coagulation. A thrombin-like enzyme, called AH143, was isolated from Agkistrodon halys venom by gel filtration on a Sephadex G-50 column, ion-exchange chromatography on a DEAE-Sepharose and high performance liquid chromatography (HPLC) on a C18 column. In the final stage of purification, 0.82 mg of purified enzyme was obtained from 182.5 mg of venom. The purified enzyme showed a single protein band by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), under reducing conditions, and its molecular mass was found to be about 30 kDa. AH143 revealed clotting activity in human plasma, which was not inhibited by EDTA or heparin. This enzyme still demonstrated coagulation activity when exposed to variations in temperature and pH ranging, respectively, from 30 to 40°C and from 7.0 to 8.0. It also displayed proteolytic activities on synthetic substrate. The purified enzyme did not show any effect on casein. We concluded that the venom of the Iranian snake Agkistrodon halys contains about 0.45% single procoagulant protein which appears to be a thrombin-like enzyme. |
format |
Article in Journal/Newspaper |
author |
M Ghorbanpur A Zare Mirakabadi F Zokaee H Zolfagarrian H Rabiei |
author_facet |
M Ghorbanpur A Zare Mirakabadi F Zokaee H Zolfagarrian H Rabiei |
author_sort |
M Ghorbanpur |
title |
Purification and partial characterization of a coagulant serine protease from the venom of the Iranian snake Agkistrodon halys |
title_short |
Purification and partial characterization of a coagulant serine protease from the venom of the Iranian snake Agkistrodon halys |
title_full |
Purification and partial characterization of a coagulant serine protease from the venom of the Iranian snake Agkistrodon halys |
title_fullStr |
Purification and partial characterization of a coagulant serine protease from the venom of the Iranian snake Agkistrodon halys |
title_full_unstemmed |
Purification and partial characterization of a coagulant serine protease from the venom of the Iranian snake Agkistrodon halys |
title_sort |
purification and partial characterization of a coagulant serine protease from the venom of the iranian snake agkistrodon halys |
publisher |
SciELO |
publishDate |
2009 |
url |
https://doi.org/10.1590/S1678-91992009000300005 https://doaj.org/article/9d6995f45b914f26a7052c3dd83c5cd2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 15, Iss 3, Pp 411-423 (2009) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300005 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992009000300005 1678-9199 https://doaj.org/article/9d6995f45b914f26a7052c3dd83c5cd2 |
op_doi |
https://doi.org/10.1590/S1678-91992009000300005 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
15 |
container_issue |
3 |
container_start_page |
411 |
op_container_end_page |
423 |
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1766339591193755648 |