Isolation, characterization and application of a lytic phage vB_VspM_VS1 against Vibrio splendidus biofilm
Vibrio splendidus is a common pathogen in the ocean that infects Apostichopus japonicus, Atlantic salmon and Crassostrea gigas, leading to a variety of diseases. In this study, a virulent phage vB_VspM_VS1, which infects V. splendidus, was isolated from aquaculture ponds in Dalian, China, and it bel...
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ftdoajarticles:oai:doaj.org/article:9d441d376f0e43e38a58b2a1a264ec85 2023-10-09T21:49:59+02:00 Isolation, characterization and application of a lytic phage vB_VspM_VS1 against Vibrio splendidus biofilm Xuemei Duan Liming Jiang Ming Guo Chenghua Li 2023-01-01T00:00:00Z https://doaj.org/article/9d441d376f0e43e38a58b2a1a264ec85 EN eng Public Library of Science (PLoS) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10473537/?tool=EBI https://doaj.org/toc/1932-6203 1932-6203 https://doaj.org/article/9d441d376f0e43e38a58b2a1a264ec85 PLoS ONE, Vol 18, Iss 9 (2023) Medicine R Science Q article 2023 ftdoajarticles 2023-09-10T00:35:54Z Vibrio splendidus is a common pathogen in the ocean that infects Apostichopus japonicus, Atlantic salmon and Crassostrea gigas, leading to a variety of diseases. In this study, a virulent phage vB_VspM_VS1, which infects V. splendidus, was isolated from aquaculture ponds in Dalian, China, and it belongs to the family Straboviridae in the order Caudoviricetes. vB_VspM_VS1 had an adsorption rate of 96% in 15 min, a latent period of 65 min, and a burst size of 140 ± 6 PFU/cell. The complete genome of phage vB_VspM_VS1 consists of a linear double-stranded DNA that is 248,270 bp in length with an average G + C content of 42.5% and 389 putative protein-coding genes; 116 genes have known functions. There are 4 tail fiber genes in the positive and negative strands of the phage vB_VspM_VS1 genome. The protein domain of the phage vB_VspM_VS1 tail fibers was obtained from the Protein Data Bank and the SMART (http://smart.embl.de) database. Bacterial challenge tests revealed that the growth of V. splendidus HS0 was apparently inhibited (OD600 < 0.01) in 12 h at an MOI of 10. In against biofilms, we also showed that the OD570 value of the vB_VspM_VS1-treated group (MOI = 1) decreased significantly to 0.04 ± 0.01 compared with that of the control group (0.48 ± 0.08) at 24 h. This study characterizes the genome of the phage vB_VspM_VS1 that infects the pathogenic bacterium V. splendidus of A. japonicus. Article in Journal/Newspaper Atlantic salmon Crassostrea gigas Directory of Open Access Journals: DOAJ Articles |
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Medicine R Science Q |
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Medicine R Science Q Xuemei Duan Liming Jiang Ming Guo Chenghua Li Isolation, characterization and application of a lytic phage vB_VspM_VS1 against Vibrio splendidus biofilm |
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Medicine R Science Q |
description |
Vibrio splendidus is a common pathogen in the ocean that infects Apostichopus japonicus, Atlantic salmon and Crassostrea gigas, leading to a variety of diseases. In this study, a virulent phage vB_VspM_VS1, which infects V. splendidus, was isolated from aquaculture ponds in Dalian, China, and it belongs to the family Straboviridae in the order Caudoviricetes. vB_VspM_VS1 had an adsorption rate of 96% in 15 min, a latent period of 65 min, and a burst size of 140 ± 6 PFU/cell. The complete genome of phage vB_VspM_VS1 consists of a linear double-stranded DNA that is 248,270 bp in length with an average G + C content of 42.5% and 389 putative protein-coding genes; 116 genes have known functions. There are 4 tail fiber genes in the positive and negative strands of the phage vB_VspM_VS1 genome. The protein domain of the phage vB_VspM_VS1 tail fibers was obtained from the Protein Data Bank and the SMART (http://smart.embl.de) database. Bacterial challenge tests revealed that the growth of V. splendidus HS0 was apparently inhibited (OD600 < 0.01) in 12 h at an MOI of 10. In against biofilms, we also showed that the OD570 value of the vB_VspM_VS1-treated group (MOI = 1) decreased significantly to 0.04 ± 0.01 compared with that of the control group (0.48 ± 0.08) at 24 h. This study characterizes the genome of the phage vB_VspM_VS1 that infects the pathogenic bacterium V. splendidus of A. japonicus. |
format |
Article in Journal/Newspaper |
author |
Xuemei Duan Liming Jiang Ming Guo Chenghua Li |
author_facet |
Xuemei Duan Liming Jiang Ming Guo Chenghua Li |
author_sort |
Xuemei Duan |
title |
Isolation, characterization and application of a lytic phage vB_VspM_VS1 against Vibrio splendidus biofilm |
title_short |
Isolation, characterization and application of a lytic phage vB_VspM_VS1 against Vibrio splendidus biofilm |
title_full |
Isolation, characterization and application of a lytic phage vB_VspM_VS1 against Vibrio splendidus biofilm |
title_fullStr |
Isolation, characterization and application of a lytic phage vB_VspM_VS1 against Vibrio splendidus biofilm |
title_full_unstemmed |
Isolation, characterization and application of a lytic phage vB_VspM_VS1 against Vibrio splendidus biofilm |
title_sort |
isolation, characterization and application of a lytic phage vb_vspm_vs1 against vibrio splendidus biofilm |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doaj.org/article/9d441d376f0e43e38a58b2a1a264ec85 |
genre |
Atlantic salmon Crassostrea gigas |
genre_facet |
Atlantic salmon Crassostrea gigas |
op_source |
PLoS ONE, Vol 18, Iss 9 (2023) |
op_relation |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10473537/?tool=EBI https://doaj.org/toc/1932-6203 1932-6203 https://doaj.org/article/9d441d376f0e43e38a58b2a1a264ec85 |
_version_ |
1779313043284426752 |