Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells
Objective: To identify the bioactive extracts from Alternanthera sessilis and investigate its cytotoxicity potential against colon cancer cells, HT-29. Methods: This study examined the effects of three parts (aerial, leaf, stem) of whole plant on HT-29 colon cancer cell lines. Three different extrac...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:9d3edd81f7ed46c0ad1f46b15cef3760 2023-05-15T15:07:19+02:00 Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells Sivapragasam Gothai Katyakyini Muniandy Norhaizan Mohd Esa Suresh Kumar Subbiah Palanisamy Arulselvan 2018-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.239427 https://doaj.org/article/9d3edd81f7ed46c0ad1f46b15cef3760 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=8;spage=394;epage=402;aulast=Gothai https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.239427 https://doaj.org/article/9d3edd81f7ed46c0ad1f46b15cef3760 Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 8, Pp 394-402 (2018) alternanthera sessilis colorectal cancer anticancer ht-29 apoptosis Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2018 ftdoajarticles https://doi.org/10.4103/2221-1691.239427 2022-12-31T01:36:48Z Objective: To identify the bioactive extracts from Alternanthera sessilis and investigate its cytotoxicity potential against colon cancer cells, HT-29. Methods: This study examined the effects of three parts (aerial, leaf, stem) of whole plant on HT-29 colon cancer cell lines. Three different extracts from the plant parts were prepared by maceration technique using 80% ethanol. The anticancer activities were determined using MTT, clonogenic, cell motility and AOPI assay. The chemical composition profiling was analyzed by GC-MS. Results: Among three plant part extracts, leaf extract greatly suppressed the growth of colon cancer cells in time and dosage-dependent manner, followed by aerial and stem. The cytotoxicity results were rationalized with clonogenic, cell motility and AO/PI assay, where extract showed the most active activity compared to aerial and stem extracts. GC-MS analysis of leaf extract showed there were various recognized anti-cancer, anti-oxidant and anti-inflammatory compounds. Conclusions: Amid the screened extracts, the leaf extract exhibits the credible cytotoxic, anti-proliferative and apoptotic activity and hence, our findings call for additional research to conclude the active compounds and their mechanisms determining the apoptotic activity. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 8 8 394 |
institution |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
alternanthera sessilis colorectal cancer anticancer ht-29 apoptosis Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
alternanthera sessilis colorectal cancer anticancer ht-29 apoptosis Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Sivapragasam Gothai Katyakyini Muniandy Norhaizan Mohd Esa Suresh Kumar Subbiah Palanisamy Arulselvan Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells |
topic_facet |
alternanthera sessilis colorectal cancer anticancer ht-29 apoptosis Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To identify the bioactive extracts from Alternanthera sessilis and investigate its cytotoxicity potential against colon cancer cells, HT-29. Methods: This study examined the effects of three parts (aerial, leaf, stem) of whole plant on HT-29 colon cancer cell lines. Three different extracts from the plant parts were prepared by maceration technique using 80% ethanol. The anticancer activities were determined using MTT, clonogenic, cell motility and AOPI assay. The chemical composition profiling was analyzed by GC-MS. Results: Among three plant part extracts, leaf extract greatly suppressed the growth of colon cancer cells in time and dosage-dependent manner, followed by aerial and stem. The cytotoxicity results were rationalized with clonogenic, cell motility and AO/PI assay, where extract showed the most active activity compared to aerial and stem extracts. GC-MS analysis of leaf extract showed there were various recognized anti-cancer, anti-oxidant and anti-inflammatory compounds. Conclusions: Amid the screened extracts, the leaf extract exhibits the credible cytotoxic, anti-proliferative and apoptotic activity and hence, our findings call for additional research to conclude the active compounds and their mechanisms determining the apoptotic activity. |
format |
Article in Journal/Newspaper |
author |
Sivapragasam Gothai Katyakyini Muniandy Norhaizan Mohd Esa Suresh Kumar Subbiah Palanisamy Arulselvan |
author_facet |
Sivapragasam Gothai Katyakyini Muniandy Norhaizan Mohd Esa Suresh Kumar Subbiah Palanisamy Arulselvan |
author_sort |
Sivapragasam Gothai |
title |
Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells |
title_short |
Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells |
title_full |
Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells |
title_fullStr |
Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells |
title_full_unstemmed |
Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells |
title_sort |
anticancer potential of alternanthera sessilis extract on ht-29 human colon cancer cells |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/2221-1691.239427 https://doaj.org/article/9d3edd81f7ed46c0ad1f46b15cef3760 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 8, Pp 394-402 (2018) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=8;spage=394;epage=402;aulast=Gothai https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.239427 https://doaj.org/article/9d3edd81f7ed46c0ad1f46b15cef3760 |
op_doi |
https://doi.org/10.4103/2221-1691.239427 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
8 |
container_issue |
8 |
container_start_page |
394 |
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1766338856211185664 |