Prevalence and genetic characterization of Dientamoeba fragilis in asymptomatic children attending daycare centers

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: Ana Paula Oliveira-Arbex, Érica Boarato David, Simone Mario Cacciò, Cátia Regina Branco da Fonseca, Joelma Gonçalves Martin, Cilmery Suemi Kurokawa, Fabio Tosini, Jayme Augusto Souza Neto, Semíramis Guimarães
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 2021
• Subjects: Dientamoeba fragilis; Prevalence; Children; PCR; Genotypes; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic
• Online Access: https://doi.org/10.1590/s1678-9946202163039; https://doaj.org/article/9d1f3410537045f5be8f673bff15c44d

ABSTRACT In order to provide additional data on the prevalence and genetic diversity of Dientamoeba fragilis in human populations, we conducted a study in children from low-income communities in Sao Paulo State, Brazil. Fecal samples from daycare center attendees up to 6 years old (n=156) and staff members (n=18) were submitted to PCR and sequencing of D. fragilis as well as to microscopic examination for the presence of other intestinal parasites. All children assessed were asymptomatic and 10.3% (16/156) were positive for D. fragilis. No worker was found to be positive. An association between Dientamoeba and coinfection with other intestinal parasites was observed. Concerning the genetic diversity, 14 and only two isolates were genotype 1 and genotype 2, respectively. Our findings outline interesting aspects: (1) asymptomatic children as carriers of Dientamoeba in communities in which environmental conditions ensure parasite transmission and, (2) association between Dientamoeba infection in young children and coinfection with other enteric parasites, reinforcing its transmission via the fecal–oral route.