Seroprevalence of leptospirosis in an endemic mixed urban and semi-urban setting-A community-based study in the district of Colombo, Sri Lanka.
Leptospirosis is endemic in Sri Lanka. There is a need for updated seroprevalence studies in endemic areas, to improve the understanding of disease dynamics, risk factors, control methods, and for clinical diagnosis. The cut-off titres for the microscopic agglutination test (MAT) for diagnosis of ac...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2020
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0008309 https://doaj.org/article/9d1386f46b7f4e358e8e8f3d51908a8f |
id |
ftdoajarticles:oai:doaj.org/article:9d1386f46b7f4e358e8e8f3d51908a8f |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:9d1386f46b7f4e358e8e8f3d51908a8f 2023-05-15T15:18:18+02:00 Seroprevalence of leptospirosis in an endemic mixed urban and semi-urban setting-A community-based study in the district of Colombo, Sri Lanka. Senaka Rajapakse Praveen N Weeratunga Krishan Balaji Kyra Charmaine Ramchandani Udani Savbhagya de Silva Shenali Avishka Ranasinghe Dinesh Gunarathne Pasindu P B Wijerathne Narmada Fernando Shiroma M Handunnetti Sumadhya Deepika Fernando 2020-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008309 https://doaj.org/article/9d1386f46b7f4e358e8e8f3d51908a8f EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008309 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008309 https://doaj.org/article/9d1386f46b7f4e358e8e8f3d51908a8f PLoS Neglected Tropical Diseases, Vol 14, Iss 5, p e0008309 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008309 2022-12-31T07:17:08Z Leptospirosis is endemic in Sri Lanka. There is a need for updated seroprevalence studies in endemic areas, to improve the understanding of disease dynamics, risk factors, control methods, and for clinical diagnosis. The cut-off titres for the microscopic agglutination test (MAT) for diagnosis of acute leptospirosis depend on community seroprevalence, and can vary based on locality and serovar. This study aimed to identify the seroprevalence, geographical determinants, and associations of seropositivity of leptospirosis in the district of Colombo in Sri Lanka, and to determine diagnostic cut-off titres for MAT in the community studied. This study utilized a stratified cluster sampling model in the Colombo district of Sri Lanka, to sample individuals living in urban and semi-urban areas. Serovar specific MAT titres were measured on recruited individuals using a panel of saprophytic (Leptospira biflexa) and 11 pathogenic Leptospira spp. serovars. Associations between environmental risk factors and MAT positivity were examined, with location mapping using GIS software. A total of 810 individuals were included. The mean age was 51.71 years (SD 14.02) with male predominance (60%). A total of 429 (53%) tested positive at a titer of 1/40 or more for the saprophytic Leptospira biflexa serovar Patoc. Pathogenic serovar MAT was positive at a titer of 1/40 or more for at least one serovar in 269 (33.2%) individuals. From the perspective of screening for clinical disease, serovar-specific cut-off titres of 1/80 for Leptospira spp. serovars Hebdomadis, Icterohaemorrhagiae, Pomona, Ratnapura and Patoc, 1/160 for serovars Pyrogenes and Cynopteri, and 1/40 for other serovars were determined, based on the 75th quartile MAT titre for each serovar. Serovar Pyrogenes (15.9%) had the highest seroprevalence, with serovars Ratnapura, Bankinang and Australis accounting for 9.9%, 9.6% and 9.3% respectively. When the proposed new cut-offs were applied, Bankinang(9.6%) Australis(9.3%), Pyrogenes(6.9%) and Ratnapura(6.9%) were the most ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Colombo ENVELOPE(-144.733,-144.733,-76.517,-76.517) Pomona ENVELOPE(-45.900,-45.900,-60.583,-60.583) PLOS Neglected Tropical Diseases 14 5 e0008309 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Senaka Rajapakse Praveen N Weeratunga Krishan Balaji Kyra Charmaine Ramchandani Udani Savbhagya de Silva Shenali Avishka Ranasinghe Dinesh Gunarathne Pasindu P B Wijerathne Narmada Fernando Shiroma M Handunnetti Sumadhya Deepika Fernando Seroprevalence of leptospirosis in an endemic mixed urban and semi-urban setting-A community-based study in the district of Colombo, Sri Lanka. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Leptospirosis is endemic in Sri Lanka. There is a need for updated seroprevalence studies in endemic areas, to improve the understanding of disease dynamics, risk factors, control methods, and for clinical diagnosis. The cut-off titres for the microscopic agglutination test (MAT) for diagnosis of acute leptospirosis depend on community seroprevalence, and can vary based on locality and serovar. This study aimed to identify the seroprevalence, geographical determinants, and associations of seropositivity of leptospirosis in the district of Colombo in Sri Lanka, and to determine diagnostic cut-off titres for MAT in the community studied. This study utilized a stratified cluster sampling model in the Colombo district of Sri Lanka, to sample individuals living in urban and semi-urban areas. Serovar specific MAT titres were measured on recruited individuals using a panel of saprophytic (Leptospira biflexa) and 11 pathogenic Leptospira spp. serovars. Associations between environmental risk factors and MAT positivity were examined, with location mapping using GIS software. A total of 810 individuals were included. The mean age was 51.71 years (SD 14.02) with male predominance (60%). A total of 429 (53%) tested positive at a titer of 1/40 or more for the saprophytic Leptospira biflexa serovar Patoc. Pathogenic serovar MAT was positive at a titer of 1/40 or more for at least one serovar in 269 (33.2%) individuals. From the perspective of screening for clinical disease, serovar-specific cut-off titres of 1/80 for Leptospira spp. serovars Hebdomadis, Icterohaemorrhagiae, Pomona, Ratnapura and Patoc, 1/160 for serovars Pyrogenes and Cynopteri, and 1/40 for other serovars were determined, based on the 75th quartile MAT titre for each serovar. Serovar Pyrogenes (15.9%) had the highest seroprevalence, with serovars Ratnapura, Bankinang and Australis accounting for 9.9%, 9.6% and 9.3% respectively. When the proposed new cut-offs were applied, Bankinang(9.6%) Australis(9.3%), Pyrogenes(6.9%) and Ratnapura(6.9%) were the most ... |
format |
Article in Journal/Newspaper |
author |
Senaka Rajapakse Praveen N Weeratunga Krishan Balaji Kyra Charmaine Ramchandani Udani Savbhagya de Silva Shenali Avishka Ranasinghe Dinesh Gunarathne Pasindu P B Wijerathne Narmada Fernando Shiroma M Handunnetti Sumadhya Deepika Fernando |
author_facet |
Senaka Rajapakse Praveen N Weeratunga Krishan Balaji Kyra Charmaine Ramchandani Udani Savbhagya de Silva Shenali Avishka Ranasinghe Dinesh Gunarathne Pasindu P B Wijerathne Narmada Fernando Shiroma M Handunnetti Sumadhya Deepika Fernando |
author_sort |
Senaka Rajapakse |
title |
Seroprevalence of leptospirosis in an endemic mixed urban and semi-urban setting-A community-based study in the district of Colombo, Sri Lanka. |
title_short |
Seroprevalence of leptospirosis in an endemic mixed urban and semi-urban setting-A community-based study in the district of Colombo, Sri Lanka. |
title_full |
Seroprevalence of leptospirosis in an endemic mixed urban and semi-urban setting-A community-based study in the district of Colombo, Sri Lanka. |
title_fullStr |
Seroprevalence of leptospirosis in an endemic mixed urban and semi-urban setting-A community-based study in the district of Colombo, Sri Lanka. |
title_full_unstemmed |
Seroprevalence of leptospirosis in an endemic mixed urban and semi-urban setting-A community-based study in the district of Colombo, Sri Lanka. |
title_sort |
seroprevalence of leptospirosis in an endemic mixed urban and semi-urban setting-a community-based study in the district of colombo, sri lanka. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008309 https://doaj.org/article/9d1386f46b7f4e358e8e8f3d51908a8f |
long_lat |
ENVELOPE(-144.733,-144.733,-76.517,-76.517) ENVELOPE(-45.900,-45.900,-60.583,-60.583) |
geographic |
Arctic Colombo Pomona |
geographic_facet |
Arctic Colombo Pomona |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 5, p e0008309 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008309 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008309 https://doaj.org/article/9d1386f46b7f4e358e8e8f3d51908a8f |
op_doi |
https://doi.org/10.1371/journal.pntd.0008309 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
5 |
container_start_page |
e0008309 |
_version_ |
1766348494949318656 |