The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive Dogs.
Visceral Leishmaniasis (VL) has spread to many urban centers worldwide. Dogs are considered the main reservoir of VL, because canine cases often precede the occurrence of human cases. Detection and euthanasia of serologically positive dogs is one of the primary VL control measures utilized in some c...
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ftdoajarticles:oai:doaj.org/article:9d0d3697e0a74ffb90343c46d911aebb 2023-05-15T15:17:08+02:00 The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive Dogs. Deborah Bittencourt Mothé Fraga Luciano Vasconcellos Pacheco Lairton Souza Borja Pétala Gardênia da Silva Estrela Tuy Leila Andrade Bastos Manuela da Silva Solcà Leila Denise Alves Ferreira Amorim Patrícia Sampaio Tavares Veras 2016-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004333 https://doaj.org/article/9d0d3697e0a74ffb90343c46d911aebb EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4701173?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004333 https://doaj.org/article/9d0d3697e0a74ffb90343c46d911aebb PLoS Neglected Tropical Diseases, Vol 10, Iss 1, p e0004333 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004333 2022-12-31T02:16:02Z Visceral Leishmaniasis (VL) has spread to many urban centers worldwide. Dogs are considered the main reservoir of VL, because canine cases often precede the occurrence of human cases. Detection and euthanasia of serologically positive dogs is one of the primary VL control measures utilized in some countries, including Brazil. Using accurate diagnostic tests can minimize one undesirable consequence of this measure, culling false-positive dogs, and reduce the maintenance of false-negative dogs in endemic areas. In December 2011, the Brazilian Ministry of Health replaced the ELISA (EIE CVL) screening method and Indirect Immunofluorescence Test (IFI CVL) confirmatory method with a new protocol using the rapid DPP CVL screening test and EIE CVL confirmatory test. A study of diagnostic accuracy of these two protocols was done by comparing their performance using serum samples collected from a random sample of 780 dogs in an endemic area of VL. All samples were evaluated by culture and real time PCR; 766 out of the 780 dogs were tested using the previous protocol (IFI CVL + EIE CVL) and all 780 were tested using the current protocol (DPP CVL + EIE CVL). Performances of both diagnostic protocols were evaluated using a latent class variable as the gold standard. The current protocol had a higher specificity (0.98 vs. 0.95) and PPV (0.83 vs. 0.70) than the previous protocol, although sensitivity of these two protocols was similar (0.73). When tested using sera from asymptomatic animals, the current protocol had a much higher PPV (0.63 vs. 0.40) than the previous protocol (although the sensitivity of either protocol was the same, 0.71). Considering a range of theoretical CVL prevalences, the projected PPVs were higher for the current protocol than for the previous protocol for each theoretical prevalence value. The findings presented herein show that the current protocol performed better than previous protocol primarily by reducing false-positive results. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Eie ENVELOPE(7.983,7.983,63.033,63.033) PLOS Neglected Tropical Diseases 10 1 e0004333 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Deborah Bittencourt Mothé Fraga Luciano Vasconcellos Pacheco Lairton Souza Borja Pétala Gardênia da Silva Estrela Tuy Leila Andrade Bastos Manuela da Silva Solcà Leila Denise Alves Ferreira Amorim Patrícia Sampaio Tavares Veras The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive Dogs. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Visceral Leishmaniasis (VL) has spread to many urban centers worldwide. Dogs are considered the main reservoir of VL, because canine cases often precede the occurrence of human cases. Detection and euthanasia of serologically positive dogs is one of the primary VL control measures utilized in some countries, including Brazil. Using accurate diagnostic tests can minimize one undesirable consequence of this measure, culling false-positive dogs, and reduce the maintenance of false-negative dogs in endemic areas. In December 2011, the Brazilian Ministry of Health replaced the ELISA (EIE CVL) screening method and Indirect Immunofluorescence Test (IFI CVL) confirmatory method with a new protocol using the rapid DPP CVL screening test and EIE CVL confirmatory test. A study of diagnostic accuracy of these two protocols was done by comparing their performance using serum samples collected from a random sample of 780 dogs in an endemic area of VL. All samples were evaluated by culture and real time PCR; 766 out of the 780 dogs were tested using the previous protocol (IFI CVL + EIE CVL) and all 780 were tested using the current protocol (DPP CVL + EIE CVL). Performances of both diagnostic protocols were evaluated using a latent class variable as the gold standard. The current protocol had a higher specificity (0.98 vs. 0.95) and PPV (0.83 vs. 0.70) than the previous protocol, although sensitivity of these two protocols was similar (0.73). When tested using sera from asymptomatic animals, the current protocol had a much higher PPV (0.63 vs. 0.40) than the previous protocol (although the sensitivity of either protocol was the same, 0.71). Considering a range of theoretical CVL prevalences, the projected PPVs were higher for the current protocol than for the previous protocol for each theoretical prevalence value. The findings presented herein show that the current protocol performed better than previous protocol primarily by reducing false-positive results. |
format |
Article in Journal/Newspaper |
author |
Deborah Bittencourt Mothé Fraga Luciano Vasconcellos Pacheco Lairton Souza Borja Pétala Gardênia da Silva Estrela Tuy Leila Andrade Bastos Manuela da Silva Solcà Leila Denise Alves Ferreira Amorim Patrícia Sampaio Tavares Veras |
author_facet |
Deborah Bittencourt Mothé Fraga Luciano Vasconcellos Pacheco Lairton Souza Borja Pétala Gardênia da Silva Estrela Tuy Leila Andrade Bastos Manuela da Silva Solcà Leila Denise Alves Ferreira Amorim Patrícia Sampaio Tavares Veras |
author_sort |
Deborah Bittencourt Mothé Fraga |
title |
The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive Dogs. |
title_short |
The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive Dogs. |
title_full |
The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive Dogs. |
title_fullStr |
The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive Dogs. |
title_full_unstemmed |
The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive Dogs. |
title_sort |
rapid test based on leishmania infantum chimeric rk28 protein improves the diagnosis of canine visceral leishmaniasis by reducing the detection of false-positive dogs. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004333 https://doaj.org/article/9d0d3697e0a74ffb90343c46d911aebb |
long_lat |
ENVELOPE(7.983,7.983,63.033,63.033) |
geographic |
Arctic Eie |
geographic_facet |
Arctic Eie |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 1, p e0004333 (2016) |
op_relation |
http://europepmc.org/articles/PMC4701173?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004333 https://doaj.org/article/9d0d3697e0a74ffb90343c46d911aebb |
op_doi |
https://doi.org/10.1371/journal.pntd.0004333 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
10 |
container_issue |
1 |
container_start_page |
e0004333 |
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