A combined human case of Dirofilaria ursi infection in dorsal subcutaneous tissue and Anisakis simplex sensu stricto (s.s.) infection in ventral subcutaneous tissue
Abstract Background Dirofilaria ursi is a filarial nematode that parasitizes the subcutaneous tissues of the American black bear (Ursus americanus) and Japanese black bear (Ursus thiabetanus japonicus). D. ursi that has parasitized black bears has the potential to subsequently infect humans. In addi...
Published in: | Tropical Medicine and Health |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2017
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s41182-017-0067-4 https://doaj.org/article/9ce7e846c8a1461587f05ab9dbbe1918 |
id |
ftdoajarticles:oai:doaj.org/article:9ce7e846c8a1461587f05ab9dbbe1918 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:9ce7e846c8a1461587f05ab9dbbe1918 2023-05-15T15:13:17+02:00 A combined human case of Dirofilaria ursi infection in dorsal subcutaneous tissue and Anisakis simplex sensu stricto (s.s.) infection in ventral subcutaneous tissue Minoru Yamada Namiko Shishito Yoshihiro Nozawa Shigehiko Uni Keisuke Nishioka Takaaki Nakaya 2017-11-01T00:00:00Z https://doi.org/10.1186/s41182-017-0067-4 https://doaj.org/article/9ce7e846c8a1461587f05ab9dbbe1918 EN eng BMC http://link.springer.com/article/10.1186/s41182-017-0067-4 https://doaj.org/toc/1349-4147 doi:10.1186/s41182-017-0067-4 1349-4147 https://doaj.org/article/9ce7e846c8a1461587f05ab9dbbe1918 Tropical Medicine and Health, Vol 45, Iss 1, Pp 1-6 (2017) Helminthic subcutaneous infections Dirofilaria ursi Anisakis simplex sensu stricto (s.s.) A combined human case Arctic medicine. Tropical medicine RC955-962 article 2017 ftdoajarticles https://doi.org/10.1186/s41182-017-0067-4 2022-12-31T10:47:31Z Abstract Background Dirofilaria ursi is a filarial nematode that parasitizes the subcutaneous tissues of the American black bear (Ursus americanus) and Japanese black bear (Ursus thiabetanus japonicus). D. ursi that has parasitized black bears has the potential to subsequently infect humans. In addition, extra-gastrointestinal anisakiasis is less common in Japan. Case presentation We report a case of ventral subcutaneous anisakiasis and dorsal subcutaneous dirofilariasis that was acquired in Fukushima, in the northern part of Japan. The patient was an 83-year-old Japanese female, and subcutaneous parasitic granulomas were present on her left abdomen (near the navel) and left scapula. A pathological examination of the surgically dissected tissue sections from each region demonstrated eosinophilic granulomas containing different species of parasites. To enable the morphological and molecular identification of these parasites, DNA was extracted from paraffin-embedded sections using DEXPAT reagent, and the cytochrome oxidase 2 (COX2), internal transcribed spacer 1 (ITS1), 5.8S and ITS2 regions of the Anisakis larvae, and the 5S rRNA region of the male Dirofilaria were sequenced. The PCR products were examined and compared with DNA databases. Molecular analysis of the COX2 and 5S rRNA sequences of each worm revealed that the nematode found in the ventral region belonged to Anisakis simplex sensu stricto (s.s.) and the male Dirofilaria found in the dorsal region was classified as D. ursi. Conclusion The present case showed a combined human case of D. ursi and A. simplex s.s. infections in subcutaneous tissues. The results of this study will contribute to the identification of unknown parasites in histological sections. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Fukushima Tropical Medicine and Health 45 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Helminthic subcutaneous infections Dirofilaria ursi Anisakis simplex sensu stricto (s.s.) A combined human case Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Helminthic subcutaneous infections Dirofilaria ursi Anisakis simplex sensu stricto (s.s.) A combined human case Arctic medicine. Tropical medicine RC955-962 Minoru Yamada Namiko Shishito Yoshihiro Nozawa Shigehiko Uni Keisuke Nishioka Takaaki Nakaya A combined human case of Dirofilaria ursi infection in dorsal subcutaneous tissue and Anisakis simplex sensu stricto (s.s.) infection in ventral subcutaneous tissue |
topic_facet |
Helminthic subcutaneous infections Dirofilaria ursi Anisakis simplex sensu stricto (s.s.) A combined human case Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract Background Dirofilaria ursi is a filarial nematode that parasitizes the subcutaneous tissues of the American black bear (Ursus americanus) and Japanese black bear (Ursus thiabetanus japonicus). D. ursi that has parasitized black bears has the potential to subsequently infect humans. In addition, extra-gastrointestinal anisakiasis is less common in Japan. Case presentation We report a case of ventral subcutaneous anisakiasis and dorsal subcutaneous dirofilariasis that was acquired in Fukushima, in the northern part of Japan. The patient was an 83-year-old Japanese female, and subcutaneous parasitic granulomas were present on her left abdomen (near the navel) and left scapula. A pathological examination of the surgically dissected tissue sections from each region demonstrated eosinophilic granulomas containing different species of parasites. To enable the morphological and molecular identification of these parasites, DNA was extracted from paraffin-embedded sections using DEXPAT reagent, and the cytochrome oxidase 2 (COX2), internal transcribed spacer 1 (ITS1), 5.8S and ITS2 regions of the Anisakis larvae, and the 5S rRNA region of the male Dirofilaria were sequenced. The PCR products were examined and compared with DNA databases. Molecular analysis of the COX2 and 5S rRNA sequences of each worm revealed that the nematode found in the ventral region belonged to Anisakis simplex sensu stricto (s.s.) and the male Dirofilaria found in the dorsal region was classified as D. ursi. Conclusion The present case showed a combined human case of D. ursi and A. simplex s.s. infections in subcutaneous tissues. The results of this study will contribute to the identification of unknown parasites in histological sections. |
format |
Article in Journal/Newspaper |
author |
Minoru Yamada Namiko Shishito Yoshihiro Nozawa Shigehiko Uni Keisuke Nishioka Takaaki Nakaya |
author_facet |
Minoru Yamada Namiko Shishito Yoshihiro Nozawa Shigehiko Uni Keisuke Nishioka Takaaki Nakaya |
author_sort |
Minoru Yamada |
title |
A combined human case of Dirofilaria ursi infection in dorsal subcutaneous tissue and Anisakis simplex sensu stricto (s.s.) infection in ventral subcutaneous tissue |
title_short |
A combined human case of Dirofilaria ursi infection in dorsal subcutaneous tissue and Anisakis simplex sensu stricto (s.s.) infection in ventral subcutaneous tissue |
title_full |
A combined human case of Dirofilaria ursi infection in dorsal subcutaneous tissue and Anisakis simplex sensu stricto (s.s.) infection in ventral subcutaneous tissue |
title_fullStr |
A combined human case of Dirofilaria ursi infection in dorsal subcutaneous tissue and Anisakis simplex sensu stricto (s.s.) infection in ventral subcutaneous tissue |
title_full_unstemmed |
A combined human case of Dirofilaria ursi infection in dorsal subcutaneous tissue and Anisakis simplex sensu stricto (s.s.) infection in ventral subcutaneous tissue |
title_sort |
combined human case of dirofilaria ursi infection in dorsal subcutaneous tissue and anisakis simplex sensu stricto (s.s.) infection in ventral subcutaneous tissue |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s41182-017-0067-4 https://doaj.org/article/9ce7e846c8a1461587f05ab9dbbe1918 |
geographic |
Arctic Fukushima |
geographic_facet |
Arctic Fukushima |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Tropical Medicine and Health, Vol 45, Iss 1, Pp 1-6 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s41182-017-0067-4 https://doaj.org/toc/1349-4147 doi:10.1186/s41182-017-0067-4 1349-4147 https://doaj.org/article/9ce7e846c8a1461587f05ab9dbbe1918 |
op_doi |
https://doi.org/10.1186/s41182-017-0067-4 |
container_title |
Tropical Medicine and Health |
container_volume |
45 |
container_issue |
1 |
_version_ |
1766343858888638464 |