Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a Brazilian village.
The relationship between poor sanitation and the parasitic infection schistosomiasis is well-known, but still rarely investigated directly and quantitatively. In a Brazilian village we correlated the spatial concentration of human fecal contamination of its main river and the prevalence of schistoso...
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ftdoajarticles:oai:doaj.org/article:9cd157898e7d48b5bfc6036a91f4db68 2023-05-15T15:15:18+02:00 Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a Brazilian village. Rafael Ponce-Terashima Amber M Koskey Mitermayer G Reis Sandra L McLellan Ronald E Blanton 2014-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003186 https://doaj.org/article/9cd157898e7d48b5bfc6036a91f4db68 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4183440?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003186 https://doaj.org/article/9cd157898e7d48b5bfc6036a91f4db68 PLoS Neglected Tropical Diseases, Vol 8, Iss 10, p e3186 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003186 2022-12-31T05:29:48Z The relationship between poor sanitation and the parasitic infection schistosomiasis is well-known, but still rarely investigated directly and quantitatively. In a Brazilian village we correlated the spatial concentration of human fecal contamination of its main river and the prevalence of schistosomiasis.We validated three bacterial markers of contamination in this population by high throughput sequencing of the 16S rRNA gene and qPCR of feces from local residents. The qPCR of genetic markers from the 16S rRNA gene of Bacteroides-Prevotella group, Bacteroides HF8 cluster, and Lachnospiraceae Lachno2 cluster as well as sequencing was performed on georeferenced samples of river water. Ninety-six percent of residents were examined for schistosomiasis.Sequence of 16S rRNA DNA from stool samples validated the relative human specificity of the HF8 and Lachno 2 fecal indicators compared to animals. The concentration of fecal contamination increased markedly along the river as it passed an increasing proportion of the population on its way downstream as did the sequence reads from bacterial families associated with human feces. Lachnospiraceae provided the most robust signal of human fecal contamination. The prevalence of schistosomiasis likewise increased downstream. Using a linear regression model, a significant correlation was demonstrated between the prevalence of S. mansoni infection and local concentration of human fecal contamination based on the Lachnospiraceae Lachno2 cluster (r2 0.53) as compared to the correlation with the general fecal marker E. coli (r2 0.28).Fecal contamination in rivers has a downstream cumulative effect. The transmission of schistosomiasis correlates with very local factors probably resulting from the distribution of human fecal contamination, the limited movement of snails, and the frequency of water contact near the home. In endemic regions, the combined use of human associated bacterial markers and GIS analysis can quantitatively identify areas with risk for schistosomiasis as well ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 10 e3186 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Rafael Ponce-Terashima Amber M Koskey Mitermayer G Reis Sandra L McLellan Ronald E Blanton Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a Brazilian village. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The relationship between poor sanitation and the parasitic infection schistosomiasis is well-known, but still rarely investigated directly and quantitatively. In a Brazilian village we correlated the spatial concentration of human fecal contamination of its main river and the prevalence of schistosomiasis.We validated three bacterial markers of contamination in this population by high throughput sequencing of the 16S rRNA gene and qPCR of feces from local residents. The qPCR of genetic markers from the 16S rRNA gene of Bacteroides-Prevotella group, Bacteroides HF8 cluster, and Lachnospiraceae Lachno2 cluster as well as sequencing was performed on georeferenced samples of river water. Ninety-six percent of residents were examined for schistosomiasis.Sequence of 16S rRNA DNA from stool samples validated the relative human specificity of the HF8 and Lachno 2 fecal indicators compared to animals. The concentration of fecal contamination increased markedly along the river as it passed an increasing proportion of the population on its way downstream as did the sequence reads from bacterial families associated with human feces. Lachnospiraceae provided the most robust signal of human fecal contamination. The prevalence of schistosomiasis likewise increased downstream. Using a linear regression model, a significant correlation was demonstrated between the prevalence of S. mansoni infection and local concentration of human fecal contamination based on the Lachnospiraceae Lachno2 cluster (r2 0.53) as compared to the correlation with the general fecal marker E. coli (r2 0.28).Fecal contamination in rivers has a downstream cumulative effect. The transmission of schistosomiasis correlates with very local factors probably resulting from the distribution of human fecal contamination, the limited movement of snails, and the frequency of water contact near the home. In endemic regions, the combined use of human associated bacterial markers and GIS analysis can quantitatively identify areas with risk for schistosomiasis as well ... |
format |
Article in Journal/Newspaper |
author |
Rafael Ponce-Terashima Amber M Koskey Mitermayer G Reis Sandra L McLellan Ronald E Blanton |
author_facet |
Rafael Ponce-Terashima Amber M Koskey Mitermayer G Reis Sandra L McLellan Ronald E Blanton |
author_sort |
Rafael Ponce-Terashima |
title |
Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a Brazilian village. |
title_short |
Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a Brazilian village. |
title_full |
Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a Brazilian village. |
title_fullStr |
Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a Brazilian village. |
title_full_unstemmed |
Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a Brazilian village. |
title_sort |
sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a brazilian village. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0003186 https://doaj.org/article/9cd157898e7d48b5bfc6036a91f4db68 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 10, p e3186 (2014) |
op_relation |
http://europepmc.org/articles/PMC4183440?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003186 https://doaj.org/article/9cd157898e7d48b5bfc6036a91f4db68 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003186 |
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PLoS Neglected Tropical Diseases |
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10 |
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e3186 |
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