Sensitivity of Bacilloscopy, Culture and ELISA Methods to Diagnose Lung Tuberculosis, in Patients From Vargas State-Venezuela
This research was conducted with the objective of evaluating sensitivity in diagnosing lung tuberculosis, through bacilloscopy, culture and ELISA methods in respiratory symptomatic patients who attended the Neumonology consultation Department from Sanitary District N° 6, La Guaira, Vargas State-Vene...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2002
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ftdoajarticles:oai:doaj.org/article:9cc617f9d67f4b5082d46fe433259f76 2023-10-01T03:54:05+02:00 Sensitivity of Bacilloscopy, Culture and ELISA Methods to Diagnose Lung Tuberculosis, in Patients From Vargas State-Venezuela J Betancourt N Ruiz P Cruces W Velásquez 2002-11-01T00:00:00Z https://doaj.org/article/9cc617f9d67f4b5082d46fe433259f76 EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://produccioncientificaluz.org/index.php/kasmera/article/view/4701 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/9cc617f9d67f4b5082d46fe433259f76 Kasmera, Vol 30, Iss 2, Pp 137-144 (2002) Tuberculosis Mycobacterium tuberculosis sensibilidad baciloscopia cultivo ELISA Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2002 ftdoajarticles 2023-09-03T00:34:54Z This research was conducted with the objective of evaluating sensitivity in diagnosing lung tuberculosis, through bacilloscopy, culture and ELISA methods in respiratory symptomatic patients who attended the Neumonology consultation Department from Sanitary District N° 6, La Guaira, Vargas State-Venezuela. To achieve this, two extended sputum samples were carried out, which were stained by the Ziehl-Neelsen method (Bacilloscopy). Each sample was sown using the method proposed by Ogawa- Kudoh. A blood sample was taken from the patients with positive sputum samples by bacilloscopy and culture methods to detect antibodies for M. tuberculosis. Out of the 200 samples analyzed to diagnose lung tuberculosis, 20 (10,0%) proved positive through the culture method, and only 11 (5,5%) were serum-positive by the ELISA method. The culture, bacilloscopy, and ELISA methods showed a sensitivity of 100, 65 and 55 % respectively. These results suggest that the Ogawa-Kudoh culture method is much more sensitive; therefore, this method gives more reliable and accurate results, especially in samples with low bacillus density. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish |
topic |
Tuberculosis Mycobacterium tuberculosis sensibilidad baciloscopia cultivo ELISA Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Tuberculosis Mycobacterium tuberculosis sensibilidad baciloscopia cultivo ELISA Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 J Betancourt N Ruiz P Cruces W Velásquez Sensitivity of Bacilloscopy, Culture and ELISA Methods to Diagnose Lung Tuberculosis, in Patients From Vargas State-Venezuela |
topic_facet |
Tuberculosis Mycobacterium tuberculosis sensibilidad baciloscopia cultivo ELISA Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
This research was conducted with the objective of evaluating sensitivity in diagnosing lung tuberculosis, through bacilloscopy, culture and ELISA methods in respiratory symptomatic patients who attended the Neumonology consultation Department from Sanitary District N° 6, La Guaira, Vargas State-Venezuela. To achieve this, two extended sputum samples were carried out, which were stained by the Ziehl-Neelsen method (Bacilloscopy). Each sample was sown using the method proposed by Ogawa- Kudoh. A blood sample was taken from the patients with positive sputum samples by bacilloscopy and culture methods to detect antibodies for M. tuberculosis. Out of the 200 samples analyzed to diagnose lung tuberculosis, 20 (10,0%) proved positive through the culture method, and only 11 (5,5%) were serum-positive by the ELISA method. The culture, bacilloscopy, and ELISA methods showed a sensitivity of 100, 65 and 55 % respectively. These results suggest that the Ogawa-Kudoh culture method is much more sensitive; therefore, this method gives more reliable and accurate results, especially in samples with low bacillus density. |
format |
Article in Journal/Newspaper |
author |
J Betancourt N Ruiz P Cruces W Velásquez |
author_facet |
J Betancourt N Ruiz P Cruces W Velásquez |
author_sort |
J Betancourt |
title |
Sensitivity of Bacilloscopy, Culture and ELISA Methods to Diagnose Lung Tuberculosis, in Patients From Vargas State-Venezuela |
title_short |
Sensitivity of Bacilloscopy, Culture and ELISA Methods to Diagnose Lung Tuberculosis, in Patients From Vargas State-Venezuela |
title_full |
Sensitivity of Bacilloscopy, Culture and ELISA Methods to Diagnose Lung Tuberculosis, in Patients From Vargas State-Venezuela |
title_fullStr |
Sensitivity of Bacilloscopy, Culture and ELISA Methods to Diagnose Lung Tuberculosis, in Patients From Vargas State-Venezuela |
title_full_unstemmed |
Sensitivity of Bacilloscopy, Culture and ELISA Methods to Diagnose Lung Tuberculosis, in Patients From Vargas State-Venezuela |
title_sort |
sensitivity of bacilloscopy, culture and elisa methods to diagnose lung tuberculosis, in patients from vargas state-venezuela |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2002 |
url |
https://doaj.org/article/9cc617f9d67f4b5082d46fe433259f76 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 30, Iss 2, Pp 137-144 (2002) |
op_relation |
https://produccioncientificaluz.org/index.php/kasmera/article/view/4701 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/9cc617f9d67f4b5082d46fe433259f76 |
_version_ |
1778521404335980544 |