Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms
ELISA was used to evaluate, accompany, and compare the humoral immune response of Swiss mice during hyperimmunization with native and Cobalt-60-irradiated (60Co) venoms of Bothrops jararaca, Bothrops jararacussu and Bothrops moojeni. Potency and neutralization were evaluated by in vitro challenges....
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/S1678-91992005000400006 https://doaj.org/article/9cb933e7837c4ac2bdff30ce7140a12a |
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ftdoajarticles:oai:doaj.org/article:9cb933e7837c4ac2bdff30ce7140a12a 2023-05-15T15:06:00+02:00 Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms R. S. Ferreira Junior N. Nascimento J. C. Martinez J. B. Alves D. A. Meira B. Barraviera 2005-12-01T00:00:00Z https://doi.org/10.1590/S1678-91992005000400006 https://doaj.org/article/9cb933e7837c4ac2bdff30ce7140a12a EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400006 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992005000400006 1678-9199 https://doaj.org/article/9cb933e7837c4ac2bdff30ce7140a12a Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 11, Iss 4, Pp 447-464 (2005) venom irradiation Bothrops ELISA serum production detoxification Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2005 ftdoajarticles https://doi.org/10.1590/S1678-91992005000400006 2022-12-31T04:29:45Z ELISA was used to evaluate, accompany, and compare the humoral immune response of Swiss mice during hyperimmunization with native and Cobalt-60-irradiated (60Co) venoms of Bothrops jararaca, Bothrops jararacussu and Bothrops moojeni. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by in vivo challenge, and the side effects were assessed. The animals immunization with one LD50 of each venom occurred on days 1, 15, 21, 30, and 45, when blood samples were collected; challenges happened on the 60th day. Results showed that ELISA was efficient in evaluating, accompanying and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom. Immunogenic capacity was maintained after 60Co-irradiation. The sera produced with native venom showed neutralizing potency and capacity similar to those of the sera produced with irradiated venom. All antibodies were able to neutralize five LD50 from these venoms. Clinical alterations were minimum during hyperimmunization with irradiated venom, however, necrosis and death occurred in animals inoculated with native venom. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 11 4 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
venom irradiation Bothrops ELISA serum production detoxification Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
venom irradiation Bothrops ELISA serum production detoxification Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 R. S. Ferreira Junior N. Nascimento J. C. Martinez J. B. Alves D. A. Meira B. Barraviera Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms |
topic_facet |
venom irradiation Bothrops ELISA serum production detoxification Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
ELISA was used to evaluate, accompany, and compare the humoral immune response of Swiss mice during hyperimmunization with native and Cobalt-60-irradiated (60Co) venoms of Bothrops jararaca, Bothrops jararacussu and Bothrops moojeni. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by in vivo challenge, and the side effects were assessed. The animals immunization with one LD50 of each venom occurred on days 1, 15, 21, 30, and 45, when blood samples were collected; challenges happened on the 60th day. Results showed that ELISA was efficient in evaluating, accompanying and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom. Immunogenic capacity was maintained after 60Co-irradiation. The sera produced with native venom showed neutralizing potency and capacity similar to those of the sera produced with irradiated venom. All antibodies were able to neutralize five LD50 from these venoms. Clinical alterations were minimum during hyperimmunization with irradiated venom, however, necrosis and death occurred in animals inoculated with native venom. |
format |
Article in Journal/Newspaper |
author |
R. S. Ferreira Junior N. Nascimento J. C. Martinez J. B. Alves D. A. Meira B. Barraviera |
author_facet |
R. S. Ferreira Junior N. Nascimento J. C. Martinez J. B. Alves D. A. Meira B. Barraviera |
author_sort |
R. S. Ferreira Junior |
title |
Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms |
title_short |
Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms |
title_full |
Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms |
title_fullStr |
Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms |
title_full_unstemmed |
Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms |
title_sort |
immunological assessment of mice hyperimmunized with native and cobalt-60-irradiated bothrops venoms |
publisher |
SciELO |
publishDate |
2005 |
url |
https://doi.org/10.1590/S1678-91992005000400006 https://doaj.org/article/9cb933e7837c4ac2bdff30ce7140a12a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 11, Iss 4, Pp 447-464 (2005) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400006 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992005000400006 1678-9199 https://doaj.org/article/9cb933e7837c4ac2bdff30ce7140a12a |
op_doi |
https://doi.org/10.1590/S1678-91992005000400006 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
11 |
container_issue |
4 |
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1766337676123832320 |