Synthetic peptides as a novel approach for detecting antibodies against sand fly saliva.
BACKGROUND:Hosts repeatedly bitten by sand flies develop antibodies against sand fly saliva and screening of these immunoglobulins can be employed to estimate the risk of Leishmania transmission, to indicate the feeding preferences of sand flies, or to evaluate the effectiveness of vector control ca...
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ftdoajarticles:oai:doaj.org/article:9ca7ef01099945c283312d0bed2f7d8e 2023-05-15T15:10:25+02:00 Synthetic peptides as a novel approach for detecting antibodies against sand fly saliva. Michal Sima Blanka Ferencova Tapan Bhattacharyya Michael A Miles Sergey V Litvinov Asrat Hailu Gad Baneth Petr Volf 2019-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007078 https://doaj.org/article/9ca7ef01099945c283312d0bed2f7d8e EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007078 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007078 https://doaj.org/article/9ca7ef01099945c283312d0bed2f7d8e PLoS Neglected Tropical Diseases, Vol 13, Iss 1, p e0007078 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007078 2022-12-31T13:13:16Z BACKGROUND:Hosts repeatedly bitten by sand flies develop antibodies against sand fly saliva and screening of these immunoglobulins can be employed to estimate the risk of Leishmania transmission, to indicate the feeding preferences of sand flies, or to evaluate the effectiveness of vector control campaigns. Previously, antibodies to sand fly saliva were detected using whole salivary gland homogenate (SGH) or recombinant proteins, both of which also have their disadvantages. This is the first study on sand flies where short peptides designed based on salivary antigens were successfully utilized for antibody screening. METHODOLOGY/PRINCIPAL FINDINGS:Specific IgG was studied in hosts naturally exposed to Phlebotomus orientalis, the main vector of Leishmania donovani in East Africa. Four peptides were designed by the commercial program EpiQuest-B, based on the sequences of the two most promising salivary antigens, yellow-related protein and ParSP25-like protein. Short amino acid peptides were synthesised and modified for ELISA experiments. Specific anti-P. orientalis IgG was detected in sera of dogs, goats, and sheep from Ethiopia. The peptide OR24 P2 was shown to be suitable for antibody screening; it correlated positively with SGH and its specificity and sensitivity were comparable or even better than that of previously published recombinant proteins. CONCLUSIONS/SIGNIFICANCE:OR24 P2, the peptide based on salivary antigen of P. orientalis, was shown to be a valuable tool for antibody screening of domestic animals naturally exposed to P. orientalis. We suggest the application of this promising methodology using species-specific short peptides to other sand fly-host combinations. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 1 e0007078 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Michal Sima Blanka Ferencova Tapan Bhattacharyya Michael A Miles Sergey V Litvinov Asrat Hailu Gad Baneth Petr Volf Synthetic peptides as a novel approach for detecting antibodies against sand fly saliva. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Hosts repeatedly bitten by sand flies develop antibodies against sand fly saliva and screening of these immunoglobulins can be employed to estimate the risk of Leishmania transmission, to indicate the feeding preferences of sand flies, or to evaluate the effectiveness of vector control campaigns. Previously, antibodies to sand fly saliva were detected using whole salivary gland homogenate (SGH) or recombinant proteins, both of which also have their disadvantages. This is the first study on sand flies where short peptides designed based on salivary antigens were successfully utilized for antibody screening. METHODOLOGY/PRINCIPAL FINDINGS:Specific IgG was studied in hosts naturally exposed to Phlebotomus orientalis, the main vector of Leishmania donovani in East Africa. Four peptides were designed by the commercial program EpiQuest-B, based on the sequences of the two most promising salivary antigens, yellow-related protein and ParSP25-like protein. Short amino acid peptides were synthesised and modified for ELISA experiments. Specific anti-P. orientalis IgG was detected in sera of dogs, goats, and sheep from Ethiopia. The peptide OR24 P2 was shown to be suitable for antibody screening; it correlated positively with SGH and its specificity and sensitivity were comparable or even better than that of previously published recombinant proteins. CONCLUSIONS/SIGNIFICANCE:OR24 P2, the peptide based on salivary antigen of P. orientalis, was shown to be a valuable tool for antibody screening of domestic animals naturally exposed to P. orientalis. We suggest the application of this promising methodology using species-specific short peptides to other sand fly-host combinations. |
format |
Article in Journal/Newspaper |
author |
Michal Sima Blanka Ferencova Tapan Bhattacharyya Michael A Miles Sergey V Litvinov Asrat Hailu Gad Baneth Petr Volf |
author_facet |
Michal Sima Blanka Ferencova Tapan Bhattacharyya Michael A Miles Sergey V Litvinov Asrat Hailu Gad Baneth Petr Volf |
author_sort |
Michal Sima |
title |
Synthetic peptides as a novel approach for detecting antibodies against sand fly saliva. |
title_short |
Synthetic peptides as a novel approach for detecting antibodies against sand fly saliva. |
title_full |
Synthetic peptides as a novel approach for detecting antibodies against sand fly saliva. |
title_fullStr |
Synthetic peptides as a novel approach for detecting antibodies against sand fly saliva. |
title_full_unstemmed |
Synthetic peptides as a novel approach for detecting antibodies against sand fly saliva. |
title_sort |
synthetic peptides as a novel approach for detecting antibodies against sand fly saliva. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007078 https://doaj.org/article/9ca7ef01099945c283312d0bed2f7d8e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 1, p e0007078 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007078 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007078 https://doaj.org/article/9ca7ef01099945c283312d0bed2f7d8e |
op_doi |
https://doi.org/10.1371/journal.pntd.0007078 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
1 |
container_start_page |
e0007078 |
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1766341459310542848 |