Drivers of ecological assembly in the hindgut of Atlantic Cod fed a macroalgal supplemented diet
Abstract It is difficult to disentangle the many variables (e.g. internal or external cues and random events) that shape the microbiota in the gastrointestinal tract of any living species. Ecological assembly processes applied to microbial communities can elucidate these drivers. In our study, farme...
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ftdoajarticles:oai:doaj.org/article:9c9cb095c2fe4694a912998be7d99b3e 2023-05-15T15:27:05+02:00 Drivers of ecological assembly in the hindgut of Atlantic Cod fed a macroalgal supplemented diet C. Keating M. Bolton-Warberg J. Hinchcliffe R. Davies S. Whelan A. H. L. Wan R. D. Fitzgerald S. J. Davies C. J. Smith U. Z. Ijaz 2022-05-01T00:00:00Z https://doi.org/10.1038/s41522-022-00296-x https://doaj.org/article/9c9cb095c2fe4694a912998be7d99b3e EN eng Nature Portfolio https://doi.org/10.1038/s41522-022-00296-x https://doaj.org/toc/2055-5008 doi:10.1038/s41522-022-00296-x 2055-5008 https://doaj.org/article/9c9cb095c2fe4694a912998be7d99b3e npj Biofilms and Microbiomes, Vol 8, Iss 1, Pp 1-13 (2022) Microbial ecology QR100-130 article 2022 ftdoajarticles https://doi.org/10.1038/s41522-022-00296-x 2022-12-30T22:35:52Z Abstract It is difficult to disentangle the many variables (e.g. internal or external cues and random events) that shape the microbiota in the gastrointestinal tract of any living species. Ecological assembly processes applied to microbial communities can elucidate these drivers. In our study, farmed Atlantic cod (Gadus morhua) were fed a diet of 10% macroalgae supplement (Ulva rigida [ULVA] or Ascophyllum nodosum [ASCO] or a non-supplemented control diet [CTRL]) over 12 weeks. We determined the influence of ecological assembly processes using a suite of null-modelling tools. We observed dissimilarity in the abundance of common OTUs over time, which was driven by deterministic assembly. The CTRL samples showed selection as a critical assembly process. While dispersal limitation was a driver of the gut microbiome for fish fed the macroalgae supplemented diet at Week 12 (i.e., ASCO and ULVA). Fish from the ASCO grouping diverged into ASCO_N (normal) and ASCO_LG (lower growth), where ASCO_LG individuals found the diet unpalatable. The recruitment of new taxa overtime was altered in the ASCO_LG fish, with the gut microbiome showing phylogenetic underdispersion (nepotistic species recruitment). Finally, the gut microbiome (CTRL and ULVA) showed increasing robustness to taxonomic disturbance over time and lower functional redundancy. This study advances our understanding of the ecological assembly and succession in the hindgut of juvenile Atlantic cod across dietary treatments. Understanding the processes driving ecological assembly in the gut microbiome, in fish research specifically, could allow us to manipulate the microbiome for improved health or resilience to disease for improved aquaculture welfare and production. Article in Journal/Newspaper atlantic cod Gadus morhua Directory of Open Access Journals: DOAJ Articles npj Biofilms and Microbiomes 8 1 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Microbial ecology QR100-130 |
spellingShingle |
Microbial ecology QR100-130 C. Keating M. Bolton-Warberg J. Hinchcliffe R. Davies S. Whelan A. H. L. Wan R. D. Fitzgerald S. J. Davies C. J. Smith U. Z. Ijaz Drivers of ecological assembly in the hindgut of Atlantic Cod fed a macroalgal supplemented diet |
topic_facet |
Microbial ecology QR100-130 |
description |
Abstract It is difficult to disentangle the many variables (e.g. internal or external cues and random events) that shape the microbiota in the gastrointestinal tract of any living species. Ecological assembly processes applied to microbial communities can elucidate these drivers. In our study, farmed Atlantic cod (Gadus morhua) were fed a diet of 10% macroalgae supplement (Ulva rigida [ULVA] or Ascophyllum nodosum [ASCO] or a non-supplemented control diet [CTRL]) over 12 weeks. We determined the influence of ecological assembly processes using a suite of null-modelling tools. We observed dissimilarity in the abundance of common OTUs over time, which was driven by deterministic assembly. The CTRL samples showed selection as a critical assembly process. While dispersal limitation was a driver of the gut microbiome for fish fed the macroalgae supplemented diet at Week 12 (i.e., ASCO and ULVA). Fish from the ASCO grouping diverged into ASCO_N (normal) and ASCO_LG (lower growth), where ASCO_LG individuals found the diet unpalatable. The recruitment of new taxa overtime was altered in the ASCO_LG fish, with the gut microbiome showing phylogenetic underdispersion (nepotistic species recruitment). Finally, the gut microbiome (CTRL and ULVA) showed increasing robustness to taxonomic disturbance over time and lower functional redundancy. This study advances our understanding of the ecological assembly and succession in the hindgut of juvenile Atlantic cod across dietary treatments. Understanding the processes driving ecological assembly in the gut microbiome, in fish research specifically, could allow us to manipulate the microbiome for improved health or resilience to disease for improved aquaculture welfare and production. |
format |
Article in Journal/Newspaper |
author |
C. Keating M. Bolton-Warberg J. Hinchcliffe R. Davies S. Whelan A. H. L. Wan R. D. Fitzgerald S. J. Davies C. J. Smith U. Z. Ijaz |
author_facet |
C. Keating M. Bolton-Warberg J. Hinchcliffe R. Davies S. Whelan A. H. L. Wan R. D. Fitzgerald S. J. Davies C. J. Smith U. Z. Ijaz |
author_sort |
C. Keating |
title |
Drivers of ecological assembly in the hindgut of Atlantic Cod fed a macroalgal supplemented diet |
title_short |
Drivers of ecological assembly in the hindgut of Atlantic Cod fed a macroalgal supplemented diet |
title_full |
Drivers of ecological assembly in the hindgut of Atlantic Cod fed a macroalgal supplemented diet |
title_fullStr |
Drivers of ecological assembly in the hindgut of Atlantic Cod fed a macroalgal supplemented diet |
title_full_unstemmed |
Drivers of ecological assembly in the hindgut of Atlantic Cod fed a macroalgal supplemented diet |
title_sort |
drivers of ecological assembly in the hindgut of atlantic cod fed a macroalgal supplemented diet |
publisher |
Nature Portfolio |
publishDate |
2022 |
url |
https://doi.org/10.1038/s41522-022-00296-x https://doaj.org/article/9c9cb095c2fe4694a912998be7d99b3e |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
npj Biofilms and Microbiomes, Vol 8, Iss 1, Pp 1-13 (2022) |
op_relation |
https://doi.org/10.1038/s41522-022-00296-x https://doaj.org/toc/2055-5008 doi:10.1038/s41522-022-00296-x 2055-5008 https://doaj.org/article/9c9cb095c2fe4694a912998be7d99b3e |
op_doi |
https://doi.org/10.1038/s41522-022-00296-x |
container_title |
npj Biofilms and Microbiomes |
container_volume |
8 |
container_issue |
1 |
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1766357541952946176 |