The complete mitochondrial genome of the pink sea fan, Eunicella verrucosa (Pallas, 1766)

• Published in: Mitochondrial DNA Part B
• Main Authors: Luke Hooper, Tom L. Jenkins, Andrew M. Griffiths, Karen A. Moore, Jamie R. Stevens
• Format: Article in Journal/Newspaper
• Language: English
• Published: Taylor & Francis Group 2021
• Subjects: sea fan; gorgonian; dna mismatch repair gene; alcyoncea; Genetics; QH426-470; Northeast Atlantic
• Online Access: https://doi.org/10.1080/23802359.2021.1994899; https://doaj.org/article/9c8cb50d7b2a4a5ba89d00ad36a05712

The pink sea fan, Eunicella verrucosa (Pallas, 1766), inhabits rocky substrates across the northeast Atlantic and the western Mediterranean. Across much of its range it has been detrimentally affected by fishing. DNA from 17 E. verrucosa specimens was amplified by phi29-induced rolling circle amplification. Following purification by sodium acetate-ethanol precipitation, the circular genomic DNA was sequenced on an Illumina MiSeq v2. Specimens originated from sites along the west coast of Ireland, southwest Wales, southwest/southern England, northwest France, southern Portugal, and the Mediterranean coast of northeast Spain. All samples had identical mitochondrial genome sequences of 19,267 bp and included 14 protein-coding genes (including the mutS gene), two ribosomal RNA subunits (12S and 16S) and one methionine tRNA gene. Two genes (nad2 and nad5) overlapped by 13 bp; all other genes were separated by non-coding intergenic regions. All protein-coding genes had the same start codon (ATG) and a TAA or TAG stop codon, except for cox1 that terminated with the incomplete stop codon T--. The mitochondrial genome of E. verrucosa (MW588805) showed 99.72% similarity with that of a related sea fan species, Eunicella cavolini, with six SNPs and a 49 bp deletion between nad5 and nad4 in E. verrucosa distinguishing the two.