Chromosomal differences between European and North American Atlantic salmon discovered by linkage mapping and supported by fluorescence in situ hybridization analysis
Abstract Background Geographical isolation has generated a distinct difference between Atlantic salmon of European and North American Atlantic origin. The European Atlantic salmon generally has 29 pairs of chromosomes and 74 chromosome arms whereas it has been reported that the North American Atlant...
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ftdoajarticles:oai:doaj.org/article:9c75542ccff446048dfed43d3c3327d3 2023-05-15T15:27:59+02:00 Chromosomal differences between European and North American Atlantic salmon discovered by linkage mapping and supported by fluorescence in situ hybridization analysis Brenna-Hansen Silje Li Jieying Kent Matthew P Boulding Elizabeth G Dominik Sonja Davidson William S Lien Sigbjørn 2012-08-01T00:00:00Z https://doi.org/10.1186/1471-2164-13-432 https://doaj.org/article/9c75542ccff446048dfed43d3c3327d3 EN eng BMC http://www.biomedcentral.com/1471-2164/13/432 https://doaj.org/toc/1471-2164 doi:10.1186/1471-2164-13-432 1471-2164 https://doaj.org/article/9c75542ccff446048dfed43d3c3327d3 BMC Genomics, Vol 13, Iss 1, p 432 (2012) Biotechnology TP248.13-248.65 Genetics QH426-470 article 2012 ftdoajarticles https://doi.org/10.1186/1471-2164-13-432 2022-12-31T11:56:55Z Abstract Background Geographical isolation has generated a distinct difference between Atlantic salmon of European and North American Atlantic origin. The European Atlantic salmon generally has 29 pairs of chromosomes and 74 chromosome arms whereas it has been reported that the North American Atlantic salmon has 27 chromosome pairs and an NF of 72. In order to predict the major chromosomal rearrangements causing these differences, we constructed a dense linkage map for Atlantic salmon of North American origin and compared it with the well-developed map for European Atlantic salmon. Results The presented male and female genetic maps for the North American subspecies of Atlantic salmon, contains 3,662 SNPs located on 27 linkage groups. The total lengths of the female and male linkage maps were 2,153 cM and 968 cM respectively, with males characteristically showing recombination only at the telomeres. We compared these maps with recently published SNP maps from European Atlantic salmon, and predicted three chromosomal reorganization events that we then tested using fluorescence in situ hybridization (FISH) analysis. The proposed rearrangements, which define the differences in the karyotypes of the North American Atlantic salmon relative to the European Atlantic salmon, include the translocation of the p arm of ssa01 to ssa23 and polymorphic fusions: ssa26 with ssa28, and ssa08 with ssa29. Conclusions This study identified major chromosomal differences between European and North American Atlantic salmon. However, while gross structural differences were significant, the order of genetic markers at the fine-resolution scale was remarkably conserved. This is a good indication that information from the International Cooperation to Sequence the Atlantic salmon Genome, which is sequencing a European Atlantic salmon, can be transferred to Atlantic salmon from North America. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles BMC Genomics 13 1 432 |
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Directory of Open Access Journals: DOAJ Articles |
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topic |
Biotechnology TP248.13-248.65 Genetics QH426-470 |
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Biotechnology TP248.13-248.65 Genetics QH426-470 Brenna-Hansen Silje Li Jieying Kent Matthew P Boulding Elizabeth G Dominik Sonja Davidson William S Lien Sigbjørn Chromosomal differences between European and North American Atlantic salmon discovered by linkage mapping and supported by fluorescence in situ hybridization analysis |
topic_facet |
Biotechnology TP248.13-248.65 Genetics QH426-470 |
description |
Abstract Background Geographical isolation has generated a distinct difference between Atlantic salmon of European and North American Atlantic origin. The European Atlantic salmon generally has 29 pairs of chromosomes and 74 chromosome arms whereas it has been reported that the North American Atlantic salmon has 27 chromosome pairs and an NF of 72. In order to predict the major chromosomal rearrangements causing these differences, we constructed a dense linkage map for Atlantic salmon of North American origin and compared it with the well-developed map for European Atlantic salmon. Results The presented male and female genetic maps for the North American subspecies of Atlantic salmon, contains 3,662 SNPs located on 27 linkage groups. The total lengths of the female and male linkage maps were 2,153 cM and 968 cM respectively, with males characteristically showing recombination only at the telomeres. We compared these maps with recently published SNP maps from European Atlantic salmon, and predicted three chromosomal reorganization events that we then tested using fluorescence in situ hybridization (FISH) analysis. The proposed rearrangements, which define the differences in the karyotypes of the North American Atlantic salmon relative to the European Atlantic salmon, include the translocation of the p arm of ssa01 to ssa23 and polymorphic fusions: ssa26 with ssa28, and ssa08 with ssa29. Conclusions This study identified major chromosomal differences between European and North American Atlantic salmon. However, while gross structural differences were significant, the order of genetic markers at the fine-resolution scale was remarkably conserved. This is a good indication that information from the International Cooperation to Sequence the Atlantic salmon Genome, which is sequencing a European Atlantic salmon, can be transferred to Atlantic salmon from North America. |
format |
Article in Journal/Newspaper |
author |
Brenna-Hansen Silje Li Jieying Kent Matthew P Boulding Elizabeth G Dominik Sonja Davidson William S Lien Sigbjørn |
author_facet |
Brenna-Hansen Silje Li Jieying Kent Matthew P Boulding Elizabeth G Dominik Sonja Davidson William S Lien Sigbjørn |
author_sort |
Brenna-Hansen Silje |
title |
Chromosomal differences between European and North American Atlantic salmon discovered by linkage mapping and supported by fluorescence in situ hybridization analysis |
title_short |
Chromosomal differences between European and North American Atlantic salmon discovered by linkage mapping and supported by fluorescence in situ hybridization analysis |
title_full |
Chromosomal differences between European and North American Atlantic salmon discovered by linkage mapping and supported by fluorescence in situ hybridization analysis |
title_fullStr |
Chromosomal differences between European and North American Atlantic salmon discovered by linkage mapping and supported by fluorescence in situ hybridization analysis |
title_full_unstemmed |
Chromosomal differences between European and North American Atlantic salmon discovered by linkage mapping and supported by fluorescence in situ hybridization analysis |
title_sort |
chromosomal differences between european and north american atlantic salmon discovered by linkage mapping and supported by fluorescence in situ hybridization analysis |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1471-2164-13-432 https://doaj.org/article/9c75542ccff446048dfed43d3c3327d3 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
BMC Genomics, Vol 13, Iss 1, p 432 (2012) |
op_relation |
http://www.biomedcentral.com/1471-2164/13/432 https://doaj.org/toc/1471-2164 doi:10.1186/1471-2164-13-432 1471-2164 https://doaj.org/article/9c75542ccff446048dfed43d3c3327d3 |
op_doi |
https://doi.org/10.1186/1471-2164-13-432 |
container_title |
BMC Genomics |
container_volume |
13 |
container_issue |
1 |
container_start_page |
432 |
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1766358368599932928 |