Distribution of clinical isolates of Candida spp. and antifungal susceptibility of high biofilm-forming Candida isolates
Abstract INTRODUCTION: The increase in the incidence of fungal infections, especially those caused by Candida albicans and other Candida species, necessitates the understanding and treatment of Candida-associated infections. In this study, we aimed to investigate the identification, distribution, an...
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ftdoajarticles:oai:doaj.org/article:9c572e4a6a41489bb6affcaa3192eca8 2023-05-15T15:10:07+02:00 Distribution of clinical isolates of Candida spp. and antifungal susceptibility of high biofilm-forming Candida isolates Gulcan Sahal Isil Seyis Bilkay https://doi.org/10.1590/0037-8682-0136-2018 https://doaj.org/article/9c572e4a6a41489bb6affcaa3192eca8 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000500644&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0136-2018 https://doaj.org/article/9c572e4a6a41489bb6affcaa3192eca8 Revista da Sociedade Brasileira de Medicina Tropical, Vol 51, Iss 5, Pp 644-650 Antifungal resistance Biofilm formation Candida albicans Non-Candida albicans Candida Species 5-Flucytosine Arctic medicine. Tropical medicine RC955-962 article ftdoajarticles https://doi.org/10.1590/0037-8682-0136-2018 2022-12-30T23:22:44Z Abstract INTRODUCTION: The increase in the incidence of fungal infections, especially those caused by Candida albicans and other Candida species, necessitates the understanding and treatment of Candida-associated infections. In this study, we aimed to investigate the identification, distribution, and biofilm formation ability of different clinical Candida isolates and evaluate the distribution and antifungal susceptibilities of high biofilm-forming (HBF) Candida isolates. METHODS: For identification, carbohydrate fermentation, carbohydrate assimilation, and ChromAgar tests were used. Biofilm formation was assessed using crystal violet binding assay, while the susceptibility to antifungal agents was determined using ATBTM Fungus 3 test kits. RESULTS: The majority of Candida species were C. parapsilosis (31.3%; 31/99) and C. tropicalis (30.3%; 30/99). C. tropicalis was found to be the most frequently isolated species among all HBF Candida species. HBF Candida isolates were more frequently isolated from vaginal swab (35.7%; 10/28), tracheal aspirate (17.9%; 5/28), and urine (17.9%; 5/28). The majority of tested isolates were resistant to itraconazole and voriconazole, whereas no isolate was deemed resistant to 5-flucytosine. CONCLUSIONS: C. tropicalis displays the highest biofilm formation ability among all the Candida species evaluated, and HBF Candida isolates were more frequently seen in vaginal swab, tracheal aspirate, and urine samples. Our findings revealed that 5-flucytosine is the most efficient antifungal agent against HBF Candida isolates. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 51 5 644 650 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antifungal resistance Biofilm formation Candida albicans Non-Candida albicans Candida Species 5-Flucytosine Arctic medicine. Tropical medicine RC955-962 |
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Antifungal resistance Biofilm formation Candida albicans Non-Candida albicans Candida Species 5-Flucytosine Arctic medicine. Tropical medicine RC955-962 Gulcan Sahal Isil Seyis Bilkay Distribution of clinical isolates of Candida spp. and antifungal susceptibility of high biofilm-forming Candida isolates |
topic_facet |
Antifungal resistance Biofilm formation Candida albicans Non-Candida albicans Candida Species 5-Flucytosine Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract INTRODUCTION: The increase in the incidence of fungal infections, especially those caused by Candida albicans and other Candida species, necessitates the understanding and treatment of Candida-associated infections. In this study, we aimed to investigate the identification, distribution, and biofilm formation ability of different clinical Candida isolates and evaluate the distribution and antifungal susceptibilities of high biofilm-forming (HBF) Candida isolates. METHODS: For identification, carbohydrate fermentation, carbohydrate assimilation, and ChromAgar tests were used. Biofilm formation was assessed using crystal violet binding assay, while the susceptibility to antifungal agents was determined using ATBTM Fungus 3 test kits. RESULTS: The majority of Candida species were C. parapsilosis (31.3%; 31/99) and C. tropicalis (30.3%; 30/99). C. tropicalis was found to be the most frequently isolated species among all HBF Candida species. HBF Candida isolates were more frequently isolated from vaginal swab (35.7%; 10/28), tracheal aspirate (17.9%; 5/28), and urine (17.9%; 5/28). The majority of tested isolates were resistant to itraconazole and voriconazole, whereas no isolate was deemed resistant to 5-flucytosine. CONCLUSIONS: C. tropicalis displays the highest biofilm formation ability among all the Candida species evaluated, and HBF Candida isolates were more frequently seen in vaginal swab, tracheal aspirate, and urine samples. Our findings revealed that 5-flucytosine is the most efficient antifungal agent against HBF Candida isolates. |
format |
Article in Journal/Newspaper |
author |
Gulcan Sahal Isil Seyis Bilkay |
author_facet |
Gulcan Sahal Isil Seyis Bilkay |
author_sort |
Gulcan Sahal |
title |
Distribution of clinical isolates of Candida spp. and antifungal susceptibility of high biofilm-forming Candida isolates |
title_short |
Distribution of clinical isolates of Candida spp. and antifungal susceptibility of high biofilm-forming Candida isolates |
title_full |
Distribution of clinical isolates of Candida spp. and antifungal susceptibility of high biofilm-forming Candida isolates |
title_fullStr |
Distribution of clinical isolates of Candida spp. and antifungal susceptibility of high biofilm-forming Candida isolates |
title_full_unstemmed |
Distribution of clinical isolates of Candida spp. and antifungal susceptibility of high biofilm-forming Candida isolates |
title_sort |
distribution of clinical isolates of candida spp. and antifungal susceptibility of high biofilm-forming candida isolates |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
url |
https://doi.org/10.1590/0037-8682-0136-2018 https://doaj.org/article/9c572e4a6a41489bb6affcaa3192eca8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 51, Iss 5, Pp 644-650 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000500644&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0136-2018 https://doaj.org/article/9c572e4a6a41489bb6affcaa3192eca8 |
op_doi |
https://doi.org/10.1590/0037-8682-0136-2018 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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51 |
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644 |
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650 |
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