Morphometric Characteristics of the Spermatozoa of Blue Fox ( Alopex lagopus ) and Silver Fox ( Vulpes vulpes )
The results presented in this study are the first such extensive characterization of the sperm morphometry of the blue fox ( Alopex lagopus ) and silver fox ( Vulpes vulpes ), as representatives of the family Canidae. Canine spermatozoa, especially the sperm of farmed foxes, are not often described...
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MDPI AG
2020
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Online Access: | https://doi.org/10.3390/ani10101927 https://doaj.org/article/9c3cce4fd1da44a7a08a623d2d69dc2d |
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ftdoajarticles:oai:doaj.org/article:9c3cce4fd1da44a7a08a623d2d69dc2d 2023-05-15T13:19:49+02:00 Morphometric Characteristics of the Spermatozoa of Blue Fox ( Alopex lagopus ) and Silver Fox ( Vulpes vulpes ) Katarzyna Andraszek Dorota Banaszewska Olga Szeleszczuk Marta Kuchta-Gładysz Anna Grzesiakowska 2020-10-01T00:00:00Z https://doi.org/10.3390/ani10101927 https://doaj.org/article/9c3cce4fd1da44a7a08a623d2d69dc2d EN eng MDPI AG https://www.mdpi.com/2076-2615/10/10/1927 https://doaj.org/toc/2076-2615 doi:10.3390/ani10101927 2076-2615 https://doaj.org/article/9c3cce4fd1da44a7a08a623d2d69dc2d Animals, Vol 10, Iss 1927, p 1927 (2020) fox sperm morphometry silver nitrate Tygerberg criteria Veterinary medicine SF600-1100 Zoology QL1-991 article 2020 ftdoajarticles https://doi.org/10.3390/ani10101927 2022-12-31T09:56:56Z The results presented in this study are the first such extensive characterization of the sperm morphometry of the blue fox ( Alopex lagopus ) and silver fox ( Vulpes vulpes ), as representatives of the family Canidae. Canine spermatozoa, especially the sperm of farmed foxes, are not often described in studies on reproduction. The aim of the study was a detailed comparison of the morphometric dimensions and shape of the sperm of two fox species: silver fox and blue fox. Semen collected from 10 silver foxes and 10 blue foxes was used for the study. The specimens were stained with silver nitrate. Measurements were performed of the length, width, perimeter, and area of the head; the area of the acrosome and its coverage; the length of the midpiece and its coverage; the length of the tail; and the length of the end piece of the tail. In addition, four head shape indices were calculated: ellipticity, elongation, roughness and regularity. The following values for the morphometric parameters and shape indices were obtained for blue fox and silver fox, respectively: head length—6.72 µm and 6.33 µm; head width—4.54.µm and 4.21 µm; head perimeter—18.11 µm and 17.37 µm; head area—21.94 µm 2 and 21.11 µm 2 acrosome area—11.50 µm 2 and 10.92 µm 2 midpiece length—12.85 µm and 12.79 µm; tail end piece length—3.44 µm and 3.28 µm; tail length—65.23 µm and 65.09 µm; acrosome coverage—52.43% and 52.83%; midpiece coverage—19.71% and 19.65%; sperm length—71.95 µm and 71.42 µm; ellipticity—1.49 and 1.52; elongation—0.19 and 0.20; roughness—0.84 and 1.88; regularity—1.09 and 0.99. The significance of differences between species was verified by Tukey’s test at p ≤ 0.05. Statistically significant differences between species were found for the following parameters: head length, width, perimeter and area; acrosome area; tail, end piece, and total sperm length; roughness and regularity. The differences in the size and shape of sperm can be used to establish reference patterns for fox sperm enabling more accurate species identification. Article in Journal/Newspaper Alopex lagopus Directory of Open Access Journals: DOAJ Articles Animals 10 10 1927 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
fox sperm morphometry silver nitrate Tygerberg criteria Veterinary medicine SF600-1100 Zoology QL1-991 |
spellingShingle |
fox sperm morphometry silver nitrate Tygerberg criteria Veterinary medicine SF600-1100 Zoology QL1-991 Katarzyna Andraszek Dorota Banaszewska Olga Szeleszczuk Marta Kuchta-Gładysz Anna Grzesiakowska Morphometric Characteristics of the Spermatozoa of Blue Fox ( Alopex lagopus ) and Silver Fox ( Vulpes vulpes ) |
topic_facet |
fox sperm morphometry silver nitrate Tygerberg criteria Veterinary medicine SF600-1100 Zoology QL1-991 |
description |
The results presented in this study are the first such extensive characterization of the sperm morphometry of the blue fox ( Alopex lagopus ) and silver fox ( Vulpes vulpes ), as representatives of the family Canidae. Canine spermatozoa, especially the sperm of farmed foxes, are not often described in studies on reproduction. The aim of the study was a detailed comparison of the morphometric dimensions and shape of the sperm of two fox species: silver fox and blue fox. Semen collected from 10 silver foxes and 10 blue foxes was used for the study. The specimens were stained with silver nitrate. Measurements were performed of the length, width, perimeter, and area of the head; the area of the acrosome and its coverage; the length of the midpiece and its coverage; the length of the tail; and the length of the end piece of the tail. In addition, four head shape indices were calculated: ellipticity, elongation, roughness and regularity. The following values for the morphometric parameters and shape indices were obtained for blue fox and silver fox, respectively: head length—6.72 µm and 6.33 µm; head width—4.54.µm and 4.21 µm; head perimeter—18.11 µm and 17.37 µm; head area—21.94 µm 2 and 21.11 µm 2 acrosome area—11.50 µm 2 and 10.92 µm 2 midpiece length—12.85 µm and 12.79 µm; tail end piece length—3.44 µm and 3.28 µm; tail length—65.23 µm and 65.09 µm; acrosome coverage—52.43% and 52.83%; midpiece coverage—19.71% and 19.65%; sperm length—71.95 µm and 71.42 µm; ellipticity—1.49 and 1.52; elongation—0.19 and 0.20; roughness—0.84 and 1.88; regularity—1.09 and 0.99. The significance of differences between species was verified by Tukey’s test at p ≤ 0.05. Statistically significant differences between species were found for the following parameters: head length, width, perimeter and area; acrosome area; tail, end piece, and total sperm length; roughness and regularity. The differences in the size and shape of sperm can be used to establish reference patterns for fox sperm enabling more accurate species identification. |
format |
Article in Journal/Newspaper |
author |
Katarzyna Andraszek Dorota Banaszewska Olga Szeleszczuk Marta Kuchta-Gładysz Anna Grzesiakowska |
author_facet |
Katarzyna Andraszek Dorota Banaszewska Olga Szeleszczuk Marta Kuchta-Gładysz Anna Grzesiakowska |
author_sort |
Katarzyna Andraszek |
title |
Morphometric Characteristics of the Spermatozoa of Blue Fox ( Alopex lagopus ) and Silver Fox ( Vulpes vulpes ) |
title_short |
Morphometric Characteristics of the Spermatozoa of Blue Fox ( Alopex lagopus ) and Silver Fox ( Vulpes vulpes ) |
title_full |
Morphometric Characteristics of the Spermatozoa of Blue Fox ( Alopex lagopus ) and Silver Fox ( Vulpes vulpes ) |
title_fullStr |
Morphometric Characteristics of the Spermatozoa of Blue Fox ( Alopex lagopus ) and Silver Fox ( Vulpes vulpes ) |
title_full_unstemmed |
Morphometric Characteristics of the Spermatozoa of Blue Fox ( Alopex lagopus ) and Silver Fox ( Vulpes vulpes ) |
title_sort |
morphometric characteristics of the spermatozoa of blue fox ( alopex lagopus ) and silver fox ( vulpes vulpes ) |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/ani10101927 https://doaj.org/article/9c3cce4fd1da44a7a08a623d2d69dc2d |
genre |
Alopex lagopus |
genre_facet |
Alopex lagopus |
op_source |
Animals, Vol 10, Iss 1927, p 1927 (2020) |
op_relation |
https://www.mdpi.com/2076-2615/10/10/1927 https://doaj.org/toc/2076-2615 doi:10.3390/ani10101927 2076-2615 https://doaj.org/article/9c3cce4fd1da44a7a08a623d2d69dc2d |
op_doi |
https://doi.org/10.3390/ani10101927 |
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Animals |
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10 |
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10 |
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1927 |
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1766349767207550976 |