Apoptotic effect on HeLa Cells produced by Chlamydia trachomatis-LPS
Abstract: The interaction between the lipopolysaccharide (LPS) of Chlamydia trachomatis and mammalian cells is still largely unknown. Chlamydia trachomatis is an obligate intracellular bacterium responsible for several diseases in humans and animals. In this work, thanks to the isolation of the lipo...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2007
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ftdoajarticles:oai:doaj.org/article:9c354772c1d844eb96373480ce82ee94 2023-10-01T03:54:22+02:00 Apoptotic effect on HeLa Cells produced by Chlamydia trachomatis-LPS Millán-Mendoza Beatriz Hakimi Hamid Eley, Adrian 2007-06-01T00:00:00Z https://doaj.org/article/9c354772c1d844eb96373480ce82ee94 EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://drive.google.com/file/d/1NVASGcHvAojwS5T3fuTx-x4UnlPW-2UB/view https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/9c354772c1d844eb96373480ce82ee94 Kasmera, Vol 35, Iss 1, Pp 7-14 (2007) Chlamydia trachomatis LPS apoptosis nuclear supravital fluorescent stain Coloración nuclear supravital fluorescente Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2007 ftdoajarticles 2023-09-03T00:44:49Z Abstract: The interaction between the lipopolysaccharide (LPS) of Chlamydia trachomatis and mammalian cells is still largely unknown. Chlamydia trachomatis is an obligate intracellular bacterium responsible for several diseases in humans and animals. In this work, thanks to the isolation of the lipopolysaccharide from two serovars of Chlamydia trachomatis (LGV1-LGV2) and using a nuclear supravital fluorescent stain (Hoechst 33258), it was possible to investigate the apoptotic effect on HeLa cells. This work shows the apoptotic effect on HeLa cells when they were exposed to C. trachomatis-LPS from two serovars at concentrations equal to or higher than 0.5 µg/mL for a period of 48h. and also the lack of cellular response in the absence of C. trachomatis-LPS or in the presence of LPS obtained from other bacteria. Additionally, the use in equal conditions of polymyxin B, known as an inhibitor of bacterial LPS, showed a decrease of the apoptotic effect in such cells indicating that the cellular response observed was produced by C. trachomatis-LPS. These results support the theory that the LPS from C. trachomatis could be responsible for the toxic effect on cervical cells infected by these bacteria. Resumen: La interacción entre el lipopolisacárido (LPS) de Chlamydia trachomatis y las células de mamíferos permanece sin ser dilucidado. Chlamydia trachomatis es una bacteria intracelular responsable de diversas enfermedades en los humanos y animales. En este trabajo mediante el aislamiento del lipopolisacárido de dos serovares de Chlamydia trachomatis (LGV1-LGV2) y usando una coloración Supravital fluorescente (Hoechst 33258) fue posible investigar la respuesta de las células HeLa. El efecto apoptótico que sufren este tipo de células fue visible cuando fueron expuestas a dicho LPS en concentraciones iguales o mayores que 0,5 µg/mL por un periodo de 48 horas, sin embargo, se observó la falta de respuesta celular en su ausencia o en presencia de LPS de otras bacterias. Adicional mente, el uso en iguales condiciones de ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Chlamydia trachomatis LPS apoptosis nuclear supravital fluorescent stain Coloración nuclear supravital fluorescente Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Chlamydia trachomatis LPS apoptosis nuclear supravital fluorescent stain Coloración nuclear supravital fluorescente Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Millán-Mendoza Beatriz Hakimi Hamid Eley, Adrian Apoptotic effect on HeLa Cells produced by Chlamydia trachomatis-LPS |
topic_facet |
Chlamydia trachomatis LPS apoptosis nuclear supravital fluorescent stain Coloración nuclear supravital fluorescente Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Abstract: The interaction between the lipopolysaccharide (LPS) of Chlamydia trachomatis and mammalian cells is still largely unknown. Chlamydia trachomatis is an obligate intracellular bacterium responsible for several diseases in humans and animals. In this work, thanks to the isolation of the lipopolysaccharide from two serovars of Chlamydia trachomatis (LGV1-LGV2) and using a nuclear supravital fluorescent stain (Hoechst 33258), it was possible to investigate the apoptotic effect on HeLa cells. This work shows the apoptotic effect on HeLa cells when they were exposed to C. trachomatis-LPS from two serovars at concentrations equal to or higher than 0.5 µg/mL for a period of 48h. and also the lack of cellular response in the absence of C. trachomatis-LPS or in the presence of LPS obtained from other bacteria. Additionally, the use in equal conditions of polymyxin B, known as an inhibitor of bacterial LPS, showed a decrease of the apoptotic effect in such cells indicating that the cellular response observed was produced by C. trachomatis-LPS. These results support the theory that the LPS from C. trachomatis could be responsible for the toxic effect on cervical cells infected by these bacteria. Resumen: La interacción entre el lipopolisacárido (LPS) de Chlamydia trachomatis y las células de mamíferos permanece sin ser dilucidado. Chlamydia trachomatis es una bacteria intracelular responsable de diversas enfermedades en los humanos y animales. En este trabajo mediante el aislamiento del lipopolisacárido de dos serovares de Chlamydia trachomatis (LGV1-LGV2) y usando una coloración Supravital fluorescente (Hoechst 33258) fue posible investigar la respuesta de las células HeLa. El efecto apoptótico que sufren este tipo de células fue visible cuando fueron expuestas a dicho LPS en concentraciones iguales o mayores que 0,5 µg/mL por un periodo de 48 horas, sin embargo, se observó la falta de respuesta celular en su ausencia o en presencia de LPS de otras bacterias. Adicional mente, el uso en iguales condiciones de ... |
format |
Article in Journal/Newspaper |
author |
Millán-Mendoza Beatriz Hakimi Hamid Eley, Adrian |
author_facet |
Millán-Mendoza Beatriz Hakimi Hamid Eley, Adrian |
author_sort |
Millán-Mendoza Beatriz |
title |
Apoptotic effect on HeLa Cells produced by Chlamydia trachomatis-LPS |
title_short |
Apoptotic effect on HeLa Cells produced by Chlamydia trachomatis-LPS |
title_full |
Apoptotic effect on HeLa Cells produced by Chlamydia trachomatis-LPS |
title_fullStr |
Apoptotic effect on HeLa Cells produced by Chlamydia trachomatis-LPS |
title_full_unstemmed |
Apoptotic effect on HeLa Cells produced by Chlamydia trachomatis-LPS |
title_sort |
apoptotic effect on hela cells produced by chlamydia trachomatis-lps |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2007 |
url |
https://doaj.org/article/9c354772c1d844eb96373480ce82ee94 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 35, Iss 1, Pp 7-14 (2007) |
op_relation |
https://drive.google.com/file/d/1NVASGcHvAojwS5T3fuTx-x4UnlPW-2UB/view https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/9c354772c1d844eb96373480ce82ee94 |
_version_ |
1778521898337959936 |