Comparing invasive effects of five foodborne bacterial pathogens in human embryonic intestine 407 cells and human ileocecum HCT-8 cells
Objective: To refine the infectious doses of enteric bacterial pathogens in animal assays and vaccine clinical trials by studying the invasion kinetics of five bacterial pathogens with human intestinal cells. Methods: Utilizing in vitro cultured cell invasion assays with gentamicin-killing step, the...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:9c1f619266d945cf96a1cb8a89d4e532 2023-05-15T15:09:36+02:00 Comparing invasive effects of five foodborne bacterial pathogens in human embryonic intestine 407 cells and human ileocecum HCT-8 cells Lan Hu Tint T. Wai 2017-10-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2017.09.004 https://doaj.org/article/9c1f619266d945cf96a1cb8a89d4e532 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169117309450 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.09.004 https://doaj.org/article/9c1f619266d945cf96a1cb8a89d4e532 Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 10, Pp 937-944 (2017) Invasion Infectious dose Foodborne bacterial pathogens Salmonella Shigella Vaccine Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2017.09.004 2022-12-31T14:28:40Z Objective: To refine the infectious doses of enteric bacterial pathogens in animal assays and vaccine clinical trials by studying the invasion kinetics of five bacterial pathogens with human intestinal cells. Methods: Utilizing in vitro cultured cell invasion assays with gentamicin-killing step, the invasive effects were analyzed in foodborne pathogens including Salmonella, Shigella, Yersinia, Escherichia coli (E. coli) O157 and opportunistic pathogens Citrobacter in human embryonic intestine 407 cells and ileocecum HCT-8 cells at multiplicities of infection (MOIs) of 0.04–4 000.00 E. coli HS served as a noninvasive control. Results: The study results showed that the bacterial invasive efficiency and the average number of internalized bacteria per host cell changed with different starting MOIs. Higher starting MOIs did not always produce more bacterial internalization. The bacterial invasion effects varied with different bacterial strains and host cell lines. E. coli O157:H7 did invade human ileocecum HCT-8 cells. Conclusions: This study shows that these bacteria possess different invasive patterns at various starting MOIs and also in different cell lines. The results could help to figure out the appropriate infectious doses of the bacteria in animal assays and in vaccine clinical trials. The bacterial invasion kinetics is also valuable in evaluating the safety and efficacy of live attenuated bacterial vaccines. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 7 10 937 944 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Invasion Infectious dose Foodborne bacterial pathogens Salmonella Shigella Vaccine Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Invasion Infectious dose Foodborne bacterial pathogens Salmonella Shigella Vaccine Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Lan Hu Tint T. Wai Comparing invasive effects of five foodborne bacterial pathogens in human embryonic intestine 407 cells and human ileocecum HCT-8 cells |
topic_facet |
Invasion Infectious dose Foodborne bacterial pathogens Salmonella Shigella Vaccine Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To refine the infectious doses of enteric bacterial pathogens in animal assays and vaccine clinical trials by studying the invasion kinetics of five bacterial pathogens with human intestinal cells. Methods: Utilizing in vitro cultured cell invasion assays with gentamicin-killing step, the invasive effects were analyzed in foodborne pathogens including Salmonella, Shigella, Yersinia, Escherichia coli (E. coli) O157 and opportunistic pathogens Citrobacter in human embryonic intestine 407 cells and ileocecum HCT-8 cells at multiplicities of infection (MOIs) of 0.04–4 000.00 E. coli HS served as a noninvasive control. Results: The study results showed that the bacterial invasive efficiency and the average number of internalized bacteria per host cell changed with different starting MOIs. Higher starting MOIs did not always produce more bacterial internalization. The bacterial invasion effects varied with different bacterial strains and host cell lines. E. coli O157:H7 did invade human ileocecum HCT-8 cells. Conclusions: This study shows that these bacteria possess different invasive patterns at various starting MOIs and also in different cell lines. The results could help to figure out the appropriate infectious doses of the bacteria in animal assays and in vaccine clinical trials. The bacterial invasion kinetics is also valuable in evaluating the safety and efficacy of live attenuated bacterial vaccines. |
format |
Article in Journal/Newspaper |
author |
Lan Hu Tint T. Wai |
author_facet |
Lan Hu Tint T. Wai |
author_sort |
Lan Hu |
title |
Comparing invasive effects of five foodborne bacterial pathogens in human embryonic intestine 407 cells and human ileocecum HCT-8 cells |
title_short |
Comparing invasive effects of five foodborne bacterial pathogens in human embryonic intestine 407 cells and human ileocecum HCT-8 cells |
title_full |
Comparing invasive effects of five foodborne bacterial pathogens in human embryonic intestine 407 cells and human ileocecum HCT-8 cells |
title_fullStr |
Comparing invasive effects of five foodborne bacterial pathogens in human embryonic intestine 407 cells and human ileocecum HCT-8 cells |
title_full_unstemmed |
Comparing invasive effects of five foodborne bacterial pathogens in human embryonic intestine 407 cells and human ileocecum HCT-8 cells |
title_sort |
comparing invasive effects of five foodborne bacterial pathogens in human embryonic intestine 407 cells and human ileocecum hct-8 cells |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2017.09.004 https://doaj.org/article/9c1f619266d945cf96a1cb8a89d4e532 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 10, Pp 937-944 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169117309450 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.09.004 https://doaj.org/article/9c1f619266d945cf96a1cb8a89d4e532 |
op_doi |
https://doi.org/10.1016/j.apjtb.2017.09.004 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
10 |
container_start_page |
937 |
op_container_end_page |
944 |
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1766340758976069632 |