Icariin plus curcumol enhances autophagy through the mTOR pathway and promotes cathepsin B-mediated pyroptosis of prostate cancer cells
Objective: To examine the effect of icariin plus curcumol on prostate cancer cells PC3 and elucidate the underlying mechanisms. Methods: We employed the Cell Counting Kit 8 assay and colony formation assay to assess cell viability and proliferation. Autophagy expression was analyzed using monodansyl...
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Wolters Kluwer Medknow Publications
2024
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ftdoajarticles:oai:doaj.org/article:9bebfecde03041e28c2d70cce39e1e46 2024-09-09T19:26:46+00:00 Icariin plus curcumol enhances autophagy through the mTOR pathway and promotes cathepsin B-mediated pyroptosis of prostate cancer cells Xu-Yun Wang Wen-Jing Xu Bo-Nan Li Tian-Song Sun Wen Sheng 2024-01-01T00:00:00Z https://doi.org/10.4103/apjtb.apjtb_649_23 https://doaj.org/article/9bebfecde03041e28c2d70cce39e1e46 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2024;volume=14;issue=2;spage=55;epage=64;aulast=Wang https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/apjtb.apjtb_649_23 https://doaj.org/article/9bebfecde03041e28c2d70cce39e1e46 Asian Pacific Journal of Tropical Biomedicine, Vol 14, Iss 2, Pp 55-64 (2024) icariin curcumol autophagy mtor cathepsin b pyroptosis prostate cancer Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2024 ftdoajarticles https://doi.org/10.4103/apjtb.apjtb_649_23 2024-08-05T17:49:46Z Objective: To examine the effect of icariin plus curcumol on prostate cancer cells PC3 and elucidate the underlying mechanisms. Methods: We employed the Cell Counting Kit 8 assay and colony formation assay to assess cell viability and proliferation. Autophagy expression was analyzed using monodansylcadaverine staining. Immunofluorescence and Western blot analyses were used to evaluate protein expressions related to autophagy, pyroptosis, and the mTOR pathway. Cellular damage was examined using the lactate dehydrogenase assay. Moreover, cathepsin B and NLRP3 were detected by co-immunoprecipitation. Results: Icariin plus curcumol led to a decrease in PC3 cell proliferation and an enhancement of autophagy. The levels of LC3-II/LC3-I and beclin-1 were increased, while the levels of p62 and mTOR were decreased after treatment with icariin plus curcumol. These changes were reversed upon overexpression of mTOR. Furthermore, 3-methyladenine resulted in a decrease in inflammatory cytokines, pyroptosis-related protein levels, and lactate dehydrogenase concentration, compared to the icariin plus curcumol group. Inhibiting cathepsin B reversed the regulatory effects of icariin plus curcumol. Conclusions: Icariin plus curcumol demonstrates great potential as a therapeutic agent for castration-resistant prostate cancer by enhancing autophagy via the mTOR pathway and promoting pyroptosis mediated by cathepsin B. These findings provide valuable insights into the molecular mechanisms underlying the therapeutic potential of icariin and curcumol for prostate cancer treatment. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 14 2 55 64 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
icariin curcumol autophagy mtor cathepsin b pyroptosis prostate cancer Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
icariin curcumol autophagy mtor cathepsin b pyroptosis prostate cancer Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Xu-Yun Wang Wen-Jing Xu Bo-Nan Li Tian-Song Sun Wen Sheng Icariin plus curcumol enhances autophagy through the mTOR pathway and promotes cathepsin B-mediated pyroptosis of prostate cancer cells |
topic_facet |
icariin curcumol autophagy mtor cathepsin b pyroptosis prostate cancer Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To examine the effect of icariin plus curcumol on prostate cancer cells PC3 and elucidate the underlying mechanisms. Methods: We employed the Cell Counting Kit 8 assay and colony formation assay to assess cell viability and proliferation. Autophagy expression was analyzed using monodansylcadaverine staining. Immunofluorescence and Western blot analyses were used to evaluate protein expressions related to autophagy, pyroptosis, and the mTOR pathway. Cellular damage was examined using the lactate dehydrogenase assay. Moreover, cathepsin B and NLRP3 were detected by co-immunoprecipitation. Results: Icariin plus curcumol led to a decrease in PC3 cell proliferation and an enhancement of autophagy. The levels of LC3-II/LC3-I and beclin-1 were increased, while the levels of p62 and mTOR were decreased after treatment with icariin plus curcumol. These changes were reversed upon overexpression of mTOR. Furthermore, 3-methyladenine resulted in a decrease in inflammatory cytokines, pyroptosis-related protein levels, and lactate dehydrogenase concentration, compared to the icariin plus curcumol group. Inhibiting cathepsin B reversed the regulatory effects of icariin plus curcumol. Conclusions: Icariin plus curcumol demonstrates great potential as a therapeutic agent for castration-resistant prostate cancer by enhancing autophagy via the mTOR pathway and promoting pyroptosis mediated by cathepsin B. These findings provide valuable insights into the molecular mechanisms underlying the therapeutic potential of icariin and curcumol for prostate cancer treatment. |
format |
Article in Journal/Newspaper |
author |
Xu-Yun Wang Wen-Jing Xu Bo-Nan Li Tian-Song Sun Wen Sheng |
author_facet |
Xu-Yun Wang Wen-Jing Xu Bo-Nan Li Tian-Song Sun Wen Sheng |
author_sort |
Xu-Yun Wang |
title |
Icariin plus curcumol enhances autophagy through the mTOR pathway and promotes cathepsin B-mediated pyroptosis of prostate cancer cells |
title_short |
Icariin plus curcumol enhances autophagy through the mTOR pathway and promotes cathepsin B-mediated pyroptosis of prostate cancer cells |
title_full |
Icariin plus curcumol enhances autophagy through the mTOR pathway and promotes cathepsin B-mediated pyroptosis of prostate cancer cells |
title_fullStr |
Icariin plus curcumol enhances autophagy through the mTOR pathway and promotes cathepsin B-mediated pyroptosis of prostate cancer cells |
title_full_unstemmed |
Icariin plus curcumol enhances autophagy through the mTOR pathway and promotes cathepsin B-mediated pyroptosis of prostate cancer cells |
title_sort |
icariin plus curcumol enhances autophagy through the mtor pathway and promotes cathepsin b-mediated pyroptosis of prostate cancer cells |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2024 |
url |
https://doi.org/10.4103/apjtb.apjtb_649_23 https://doaj.org/article/9bebfecde03041e28c2d70cce39e1e46 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 14, Iss 2, Pp 55-64 (2024) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2024;volume=14;issue=2;spage=55;epage=64;aulast=Wang https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/apjtb.apjtb_649_23 https://doaj.org/article/9bebfecde03041e28c2d70cce39e1e46 |
op_doi |
https://doi.org/10.4103/apjtb.apjtb_649_23 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
14 |
container_issue |
2 |
container_start_page |
55 |
op_container_end_page |
64 |
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1809896324718395392 |