Glutamine ameliorates Bungarus multicinctus venom-induced lung and heart injury through HSP70: NF-κB p65 and P53/PUMA signaling pathways involved
Abstract Background: Bungarus multicinctus is one of the most dangerous venomous snakes prone to cardiopulmonary damage with extremely high mortality. In our previous work, we found that glutamine (Gln) and glutamine synthetase (GS) in pig serum were significantly reduced after Bungarus multicinctus...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
SciELO
2023
|
Subjects: | |
Online Access: | https://doi.org/10.1590/1678-9199-jvatitd-2022-0080 https://doaj.org/article/9bdbf848b076487dbb9d8f40c063c263 |
id |
ftdoajarticles:oai:doaj.org/article:9bdbf848b076487dbb9d8f40c063c263 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:9bdbf848b076487dbb9d8f40c063c263 2023-07-30T04:02:08+02:00 Glutamine ameliorates Bungarus multicinctus venom-induced lung and heart injury through HSP70: NF-κB p65 and P53/PUMA signaling pathways involved Yalan Li Zhezhe Guan Shaocong Hu Zhi Huang Dongling He Xiaoyang Cheng Tianlin Song Caifeng Mo Manqi Xiao Yue Huang Yuanmei Wei Yi Zhou Xuerong Zhang Ming Liao 2023-07-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-2022-0080 https://doaj.org/article/9bdbf848b076487dbb9d8f40c063c263 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992023000100308&lng=en&tlng=en http://www.scielo.br/pdf/jvatitd/v29/1678-9199-jvatitd-29-e20220080.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2022-0080 https://doaj.org/article/9bdbf848b076487dbb9d8f40c063c263 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 29 (2023) Glutamine Bungarus multicinctus bite HSP70 NF-κB p65 P53/PUMA Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2023 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2022-0080 2023-07-16T00:37:36Z Abstract Background: Bungarus multicinctus is one of the most dangerous venomous snakes prone to cardiopulmonary damage with extremely high mortality. In our previous work, we found that glutamine (Gln) and glutamine synthetase (GS) in pig serum were significantly reduced after Bungarus multicinctus bite. In the present study, to explore whether there is a link between the pathogenesis of cardiopulmonary injury and Gln metabolic changes induced by Bungarus multicinctus venom. We investigated the effect of Gln supplementation on the lung and heart function after snakebite. Methods: We supplemented different concentrations of Gln to mice that were envenomated by Bungarus multicinctus to observe the biological behavior, survival rate, hematological and pathological changes. Gln was supplemented immediately or one hour after the venom injection, and then changes in Gln metabolism were analyzed. Subsequently, to further explore the protective mechanism of glutamine on tissue damage, we measured the expression of heat-shock protein70 (HSP70), NF-κB P65, P53/PUMA by western blotting and real-time polymerase in the lung and heart. Results: Gln supplementation delayed the envenoming symptoms, reduced mortality, and alleviated the histopathological changes in the heart and lung of mice bitten by Bungarus multicinctus. Additionally, Gln increased the activity of glutamine synthetase (GS), glutamate dehydrogenase (GDH) and glutaminase (GLS) in serum. It also balanced the transporter SLC7A11 expression in heart and lung tissues. Bungarus multicinctus venom induced the NF-κB nuclear translocation in the lung, while the HO-1 expression was suppressed. At the same time, venom activated the P53/PUMA signaling pathway and the BAX expression in the heart. Gln treatment reversed the above phenomenon and increased HSP70 expression. Conclusion: Gln alleviated the glutamine metabolism disorder and cardiopulmonary damage caused by Bungarus multicinctus venom. It may protect lungs and heart against venom by promoting the expression of ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 29 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Glutamine Bungarus multicinctus bite HSP70 NF-κB p65 P53/PUMA Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Glutamine Bungarus multicinctus bite HSP70 NF-κB p65 P53/PUMA Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Yalan Li Zhezhe Guan Shaocong Hu Zhi Huang Dongling He Xiaoyang Cheng Tianlin Song Caifeng Mo Manqi Xiao Yue Huang Yuanmei Wei Yi Zhou Xuerong Zhang Ming Liao Glutamine ameliorates Bungarus multicinctus venom-induced lung and heart injury through HSP70: NF-κB p65 and P53/PUMA signaling pathways involved |
topic_facet |
Glutamine Bungarus multicinctus bite HSP70 NF-κB p65 P53/PUMA Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: Bungarus multicinctus is one of the most dangerous venomous snakes prone to cardiopulmonary damage with extremely high mortality. In our previous work, we found that glutamine (Gln) and glutamine synthetase (GS) in pig serum were significantly reduced after Bungarus multicinctus bite. In the present study, to explore whether there is a link between the pathogenesis of cardiopulmonary injury and Gln metabolic changes induced by Bungarus multicinctus venom. We investigated the effect of Gln supplementation on the lung and heart function after snakebite. Methods: We supplemented different concentrations of Gln to mice that were envenomated by Bungarus multicinctus to observe the biological behavior, survival rate, hematological and pathological changes. Gln was supplemented immediately or one hour after the venom injection, and then changes in Gln metabolism were analyzed. Subsequently, to further explore the protective mechanism of glutamine on tissue damage, we measured the expression of heat-shock protein70 (HSP70), NF-κB P65, P53/PUMA by western blotting and real-time polymerase in the lung and heart. Results: Gln supplementation delayed the envenoming symptoms, reduced mortality, and alleviated the histopathological changes in the heart and lung of mice bitten by Bungarus multicinctus. Additionally, Gln increased the activity of glutamine synthetase (GS), glutamate dehydrogenase (GDH) and glutaminase (GLS) in serum. It also balanced the transporter SLC7A11 expression in heart and lung tissues. Bungarus multicinctus venom induced the NF-κB nuclear translocation in the lung, while the HO-1 expression was suppressed. At the same time, venom activated the P53/PUMA signaling pathway and the BAX expression in the heart. Gln treatment reversed the above phenomenon and increased HSP70 expression. Conclusion: Gln alleviated the glutamine metabolism disorder and cardiopulmonary damage caused by Bungarus multicinctus venom. It may protect lungs and heart against venom by promoting the expression of ... |
format |
Article in Journal/Newspaper |
author |
Yalan Li Zhezhe Guan Shaocong Hu Zhi Huang Dongling He Xiaoyang Cheng Tianlin Song Caifeng Mo Manqi Xiao Yue Huang Yuanmei Wei Yi Zhou Xuerong Zhang Ming Liao |
author_facet |
Yalan Li Zhezhe Guan Shaocong Hu Zhi Huang Dongling He Xiaoyang Cheng Tianlin Song Caifeng Mo Manqi Xiao Yue Huang Yuanmei Wei Yi Zhou Xuerong Zhang Ming Liao |
author_sort |
Yalan Li |
title |
Glutamine ameliorates Bungarus multicinctus venom-induced lung and heart injury through HSP70: NF-κB p65 and P53/PUMA signaling pathways involved |
title_short |
Glutamine ameliorates Bungarus multicinctus venom-induced lung and heart injury through HSP70: NF-κB p65 and P53/PUMA signaling pathways involved |
title_full |
Glutamine ameliorates Bungarus multicinctus venom-induced lung and heart injury through HSP70: NF-κB p65 and P53/PUMA signaling pathways involved |
title_fullStr |
Glutamine ameliorates Bungarus multicinctus venom-induced lung and heart injury through HSP70: NF-κB p65 and P53/PUMA signaling pathways involved |
title_full_unstemmed |
Glutamine ameliorates Bungarus multicinctus venom-induced lung and heart injury through HSP70: NF-κB p65 and P53/PUMA signaling pathways involved |
title_sort |
glutamine ameliorates bungarus multicinctus venom-induced lung and heart injury through hsp70: nf-κb p65 and p53/puma signaling pathways involved |
publisher |
SciELO |
publishDate |
2023 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2022-0080 https://doaj.org/article/9bdbf848b076487dbb9d8f40c063c263 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 29 (2023) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992023000100308&lng=en&tlng=en http://www.scielo.br/pdf/jvatitd/v29/1678-9199-jvatitd-29-e20220080.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2022-0080 https://doaj.org/article/9bdbf848b076487dbb9d8f40c063c263 |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2022-0080 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
29 |
_version_ |
1772812852561707008 |