BAFF mediates splenic B cell response and antibody production in experimental Chagas disease.
BACKGROUND: B cells and antibodies are involved not only in controlling the spread of blood circulating Trypanosoma cruzi, but also in the autoreactive manifestations observed in Chagas disease. Acute infection results in polyclonal B cell activation associated with hypergammaglobulinemia, delayed s...
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ftdoajarticles:oai:doaj.org/article:9ba8c71ea82e40ee9454fdbbde9c9f8a 2023-05-15T15:08:54+02:00 BAFF mediates splenic B cell response and antibody production in experimental Chagas disease. Daniela A Bermejo María C Amezcua-Vesely Carolina L Montes María C Merino Ricardo C Gehrau Hugo Cejas Eva V Acosta-Rodríguez Adriana Gruppi 2010-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000679 https://doaj.org/article/9ba8c71ea82e40ee9454fdbbde9c9f8a EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2864296?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000679 https://doaj.org/article/9ba8c71ea82e40ee9454fdbbde9c9f8a PLoS Neglected Tropical Diseases, Vol 4, Iss 5, p e679 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000679 2022-12-31T03:43:39Z BACKGROUND: B cells and antibodies are involved not only in controlling the spread of blood circulating Trypanosoma cruzi, but also in the autoreactive manifestations observed in Chagas disease. Acute infection results in polyclonal B cell activation associated with hypergammaglobulinemia, delayed specific humoral immunity and high levels of non-parasite specific antibodies. Since TNF superfamily B lymphocyte Stimulator (BAFF) mediates polyclonal B cell response in vitro triggered by T. cruzi antigens, and BAFF-Tg mice show similar signs to T. cruzi infected mice, we hypothesized that BAFF can mediate polyclonal B cell response in experimental Chagas disease. METHODOLOGY/PRINCIPAL FINDINGS: BAFF is produced early and persists throughout the infection. To analyze BAFF role in experimental Chagas disease, Balb/c infected mice were injected with BR3:Fc, a soluble receptor of BAFF, to block BAFF activity. By BAFF blockade we observed that this cytokine mediates the mature B cell response and the production of non-parasite specific IgM and IgG. BAFF also influences the development of antinuclear IgG and parasite-specific IgM response, not affecting T. cruzi-specific IgG and parasitemia. Interestingly, BAFF inhibition favors the parasitism in heart. CONCLUSIONS/SIGNIFICANCE: Our results demonstrate, for the first time, an active role for BAFF in shaping the mature B cell repertoire in a parasite infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 5 e679 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Daniela A Bermejo María C Amezcua-Vesely Carolina L Montes María C Merino Ricardo C Gehrau Hugo Cejas Eva V Acosta-Rodríguez Adriana Gruppi BAFF mediates splenic B cell response and antibody production in experimental Chagas disease. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: B cells and antibodies are involved not only in controlling the spread of blood circulating Trypanosoma cruzi, but also in the autoreactive manifestations observed in Chagas disease. Acute infection results in polyclonal B cell activation associated with hypergammaglobulinemia, delayed specific humoral immunity and high levels of non-parasite specific antibodies. Since TNF superfamily B lymphocyte Stimulator (BAFF) mediates polyclonal B cell response in vitro triggered by T. cruzi antigens, and BAFF-Tg mice show similar signs to T. cruzi infected mice, we hypothesized that BAFF can mediate polyclonal B cell response in experimental Chagas disease. METHODOLOGY/PRINCIPAL FINDINGS: BAFF is produced early and persists throughout the infection. To analyze BAFF role in experimental Chagas disease, Balb/c infected mice were injected with BR3:Fc, a soluble receptor of BAFF, to block BAFF activity. By BAFF blockade we observed that this cytokine mediates the mature B cell response and the production of non-parasite specific IgM and IgG. BAFF also influences the development of antinuclear IgG and parasite-specific IgM response, not affecting T. cruzi-specific IgG and parasitemia. Interestingly, BAFF inhibition favors the parasitism in heart. CONCLUSIONS/SIGNIFICANCE: Our results demonstrate, for the first time, an active role for BAFF in shaping the mature B cell repertoire in a parasite infection. |
format |
Article in Journal/Newspaper |
author |
Daniela A Bermejo María C Amezcua-Vesely Carolina L Montes María C Merino Ricardo C Gehrau Hugo Cejas Eva V Acosta-Rodríguez Adriana Gruppi |
author_facet |
Daniela A Bermejo María C Amezcua-Vesely Carolina L Montes María C Merino Ricardo C Gehrau Hugo Cejas Eva V Acosta-Rodríguez Adriana Gruppi |
author_sort |
Daniela A Bermejo |
title |
BAFF mediates splenic B cell response and antibody production in experimental Chagas disease. |
title_short |
BAFF mediates splenic B cell response and antibody production in experimental Chagas disease. |
title_full |
BAFF mediates splenic B cell response and antibody production in experimental Chagas disease. |
title_fullStr |
BAFF mediates splenic B cell response and antibody production in experimental Chagas disease. |
title_full_unstemmed |
BAFF mediates splenic B cell response and antibody production in experimental Chagas disease. |
title_sort |
baff mediates splenic b cell response and antibody production in experimental chagas disease. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pntd.0000679 https://doaj.org/article/9ba8c71ea82e40ee9454fdbbde9c9f8a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 4, Iss 5, p e679 (2010) |
op_relation |
http://europepmc.org/articles/PMC2864296?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000679 https://doaj.org/article/9ba8c71ea82e40ee9454fdbbde9c9f8a |
op_doi |
https://doi.org/10.1371/journal.pntd.0000679 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
4 |
container_issue |
5 |
container_start_page |
e679 |
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1766340166536921088 |