Antioxidant and anti-melanogenic activities of ultrasonic extract from Stichopus japonicus
Objective: To investigate the antioxidant and anti-melanogenesis activities of an ultrasonic extract of red sea cucumber, Stichopus japonicus, collected from Jeju Island. Methods: Antioxidant activity experiments were assessed by an electron spin resonance system and a cellular model of immortalized...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2020
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ftdoajarticles:oai:doaj.org/article:9ba6cc03fb43404687b0643b48f7c724 2023-05-15T15:14:08+02:00 Antioxidant and anti-melanogenic activities of ultrasonic extract from Stichopus japonicus Yuling Ding Chanipa Jiratchayamaethasakul Junseong Kim Eun-A Kim Soo-Jin Heo Seung-Hong Lee 2020-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.273092 https://doaj.org/article/9ba6cc03fb43404687b0643b48f7c724 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=1;spage=33;epage=41;aulast=Ding https://doaj.org/toc/2588-9222 2588-9222 doi:10.4103/2221-1691.273092 https://doaj.org/article/9ba6cc03fb43404687b0643b48f7c724 Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 1, Pp 33-41 (2020) red sea cucumber (stichopus japonicus) ultrasonic extract antioxidant activity anti-melanogenesis cosmeceuticals Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.4103/2221-1691.273092 2022-12-31T12:36:59Z Objective: To investigate the antioxidant and anti-melanogenesis activities of an ultrasonic extract of red sea cucumber, Stichopus japonicus, collected from Jeju Island. Methods: Antioxidant activity experiments were assessed by an electron spin resonance system and a cellular model of immortalized human keratinocytes (HaCaT) to determine its radical scavenging activity and protective effects against 2,2’-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress. Anti-melanogenic activity of the ultrasonic extract of red sea cucumber was also examined using the melanoma cell model B16F10 and mushroom tyrosinase. Following the induction by α-melanocyte-stimulating hormone, the effects of the ultrasonic extract of red sea cucumber on intracellular tyrosinase activity, melanin content and the melanogenic protein expression of microphthalmia-associated transcription factor, tyrosinase, and tyrosinase-related proteins (TRP-1, and TRP-2) were examined. Results: The ultrasonic extract of red sea cucumber significantly scavenged 2,2-diphenyl-1-picrylhydrazyl and alkyl radicals [IC50: (0.924±0.035) and (0.327±0.006) mg/mL, respectively], as well as showed a protective effect against oxidative stress and attenuated generation of intracellular reactive oxygen species on AAPH- induced HaCaT cells, with no cytotoxicity (12.5-400 μg/mL). The ultrasonic extract of red sea cucumber also exhibited a tyrosinase inhibitory effect [IC50: (2.750±0.006) mg/mL]. On α-melanocyte-stimulating hormone-stimulated B16F10 melanoma cells, the ultrasonic extract of red sea cucumber (25-200 μg/mL) significantly inhibited not only melanin synthesis and tyrosinase activity, but also protein expressions of microphthalmia-associated transcriptional factor, tyrosinase, TRP-1, and TRP-2. Conclusions: The ultrasonic extract of red sea cucumber shows antioxidant and anti-melanogenic potential and may be a natural candidate for anti-aging as well as a whitening agent in the cosmeceuticals industry. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 10 1 33 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
red sea cucumber (stichopus japonicus) ultrasonic extract antioxidant activity anti-melanogenesis cosmeceuticals Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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red sea cucumber (stichopus japonicus) ultrasonic extract antioxidant activity anti-melanogenesis cosmeceuticals Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Yuling Ding Chanipa Jiratchayamaethasakul Junseong Kim Eun-A Kim Soo-Jin Heo Seung-Hong Lee Antioxidant and anti-melanogenic activities of ultrasonic extract from Stichopus japonicus |
topic_facet |
red sea cucumber (stichopus japonicus) ultrasonic extract antioxidant activity anti-melanogenesis cosmeceuticals Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the antioxidant and anti-melanogenesis activities of an ultrasonic extract of red sea cucumber, Stichopus japonicus, collected from Jeju Island. Methods: Antioxidant activity experiments were assessed by an electron spin resonance system and a cellular model of immortalized human keratinocytes (HaCaT) to determine its radical scavenging activity and protective effects against 2,2’-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress. Anti-melanogenic activity of the ultrasonic extract of red sea cucumber was also examined using the melanoma cell model B16F10 and mushroom tyrosinase. Following the induction by α-melanocyte-stimulating hormone, the effects of the ultrasonic extract of red sea cucumber on intracellular tyrosinase activity, melanin content and the melanogenic protein expression of microphthalmia-associated transcription factor, tyrosinase, and tyrosinase-related proteins (TRP-1, and TRP-2) were examined. Results: The ultrasonic extract of red sea cucumber significantly scavenged 2,2-diphenyl-1-picrylhydrazyl and alkyl radicals [IC50: (0.924±0.035) and (0.327±0.006) mg/mL, respectively], as well as showed a protective effect against oxidative stress and attenuated generation of intracellular reactive oxygen species on AAPH- induced HaCaT cells, with no cytotoxicity (12.5-400 μg/mL). The ultrasonic extract of red sea cucumber also exhibited a tyrosinase inhibitory effect [IC50: (2.750±0.006) mg/mL]. On α-melanocyte-stimulating hormone-stimulated B16F10 melanoma cells, the ultrasonic extract of red sea cucumber (25-200 μg/mL) significantly inhibited not only melanin synthesis and tyrosinase activity, but also protein expressions of microphthalmia-associated transcriptional factor, tyrosinase, TRP-1, and TRP-2. Conclusions: The ultrasonic extract of red sea cucumber shows antioxidant and anti-melanogenic potential and may be a natural candidate for anti-aging as well as a whitening agent in the cosmeceuticals industry. |
format |
Article in Journal/Newspaper |
author |
Yuling Ding Chanipa Jiratchayamaethasakul Junseong Kim Eun-A Kim Soo-Jin Heo Seung-Hong Lee |
author_facet |
Yuling Ding Chanipa Jiratchayamaethasakul Junseong Kim Eun-A Kim Soo-Jin Heo Seung-Hong Lee |
author_sort |
Yuling Ding |
title |
Antioxidant and anti-melanogenic activities of ultrasonic extract from Stichopus japonicus |
title_short |
Antioxidant and anti-melanogenic activities of ultrasonic extract from Stichopus japonicus |
title_full |
Antioxidant and anti-melanogenic activities of ultrasonic extract from Stichopus japonicus |
title_fullStr |
Antioxidant and anti-melanogenic activities of ultrasonic extract from Stichopus japonicus |
title_full_unstemmed |
Antioxidant and anti-melanogenic activities of ultrasonic extract from Stichopus japonicus |
title_sort |
antioxidant and anti-melanogenic activities of ultrasonic extract from stichopus japonicus |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2020 |
url |
https://doi.org/10.4103/2221-1691.273092 https://doaj.org/article/9ba6cc03fb43404687b0643b48f7c724 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 1, Pp 33-41 (2020) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=1;spage=33;epage=41;aulast=Ding https://doaj.org/toc/2588-9222 2588-9222 doi:10.4103/2221-1691.273092 https://doaj.org/article/9ba6cc03fb43404687b0643b48f7c724 |
op_doi |
https://doi.org/10.4103/2221-1691.273092 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
10 |
container_issue |
1 |
container_start_page |
33 |
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1766344619789910016 |