Venom complexity of Bothrops atrox (common lancehead) siblings
Abstract Background: Variability in snake venoms is a well-studied phenomenon. However, sex-based variation of Bothrops atrox snake venom using siblings is poorly investigated. Bothrops atrox is responsible for the majority of snakebite accidents in the Brazilian Amazon region. Differences in the ve...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/1678-9199-jvatitd-2020-0018 https://doaj.org/article/9b835479f1b14d86be995d42e5bb0391 |
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ftdoajarticles:oai:doaj.org/article:9b835479f1b14d86be995d42e5bb0391 2023-05-15T15:15:51+02:00 Venom complexity of Bothrops atrox (common lancehead) siblings Daniela Miki Hatakeyama Lídia Jorge Tasima Cesar Adolfo Bravo-Tobar Caroline Serino-Silva Alexandre Keiji Tashima Caroline Fabri Bittencourt Rodrigues Weslei da Silva Aguiar Nathália da Costa Galizio Eduardo Oliveira Venancio de Lima Victor Koiti Kavazoi Juan David Gutierrez-Marín Iasmim Baptista de Farias Sávio Stefanini Sant’Anna Kathleen Fernandes Grego Karen de Morais-Zani Anita Mitico Tanaka-Azevedo 2020-10-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-2020-0018 https://doaj.org/article/9b835479f1b14d86be995d42e5bb0391 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100334&tlng=en http://www.scielo.br/pdf/jvatitd/v26/1678-9199-jvatitd-26-e20200018.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2020-0018 https://doaj.org/article/9b835479f1b14d86be995d42e5bb0391 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 26 (2020) Bothrops atrox Snake venom Individual variation Envenomation Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2020 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2020-0018 2022-12-31T15:14:32Z Abstract Background: Variability in snake venoms is a well-studied phenomenon. However, sex-based variation of Bothrops atrox snake venom using siblings is poorly investigated. Bothrops atrox is responsible for the majority of snakebite accidents in the Brazilian Amazon region. Differences in the venom composition of Bothrops genus have been linked to several factors such as ontogeny, geographical distribution, prey preferences and sex. Thus, in the current study, venom samples of Bothrops atrox male and female siblings were analyzed in order to compare their biochemical and biological characteristics. Methods: Venoms were collected from five females and four males born from a snake captured from the wild in São Bento (Maranhão, Brazil), and kept in the Laboratory of Herpetology of Butantan Intitute. The venoms were analyzed individually and as a pool of each gender. The assays consisted in protein quantification, 1-DE, mass spectrometry, proteolytic, phospholipase A2, L-amino acid oxidase activities, minimum coagulant dose upon plasma, minimum hemorrhagic dose and lethal dose 50%. Results: Electrophoretic profiles of male’s and female’s venom pools were quite similar, with minor sex-based variation. Male venom showed higher LAAO, PLA2 and hemorrhagic activities, while female venom showed higher coagulant activity. On the other hand, the proteolytic activities did not show statistical differences between pools, although some individual variations were observed. Meanwhile, proteomic profile revealed 112 different protein compounds; of which 105 were common proteins of female’s and male’s venom pools and seven were unique to females. Despite individual variations, lethality of both pools showed similar values. Conclusion: Although differences between female and male venoms were observed, our results show that individual variations are significant even between siblings, highlighting that biological activities of venoms and its composition are influenced by other factors beyond gender. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 26 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Bothrops atrox Snake venom Individual variation Envenomation Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Bothrops atrox Snake venom Individual variation Envenomation Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Daniela Miki Hatakeyama Lídia Jorge Tasima Cesar Adolfo Bravo-Tobar Caroline Serino-Silva Alexandre Keiji Tashima Caroline Fabri Bittencourt Rodrigues Weslei da Silva Aguiar Nathália da Costa Galizio Eduardo Oliveira Venancio de Lima Victor Koiti Kavazoi Juan David Gutierrez-Marín Iasmim Baptista de Farias Sávio Stefanini Sant’Anna Kathleen Fernandes Grego Karen de Morais-Zani Anita Mitico Tanaka-Azevedo Venom complexity of Bothrops atrox (common lancehead) siblings |
topic_facet |
Bothrops atrox Snake venom Individual variation Envenomation Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: Variability in snake venoms is a well-studied phenomenon. However, sex-based variation of Bothrops atrox snake venom using siblings is poorly investigated. Bothrops atrox is responsible for the majority of snakebite accidents in the Brazilian Amazon region. Differences in the venom composition of Bothrops genus have been linked to several factors such as ontogeny, geographical distribution, prey preferences and sex. Thus, in the current study, venom samples of Bothrops atrox male and female siblings were analyzed in order to compare their biochemical and biological characteristics. Methods: Venoms were collected from five females and four males born from a snake captured from the wild in São Bento (Maranhão, Brazil), and kept in the Laboratory of Herpetology of Butantan Intitute. The venoms were analyzed individually and as a pool of each gender. The assays consisted in protein quantification, 1-DE, mass spectrometry, proteolytic, phospholipase A2, L-amino acid oxidase activities, minimum coagulant dose upon plasma, minimum hemorrhagic dose and lethal dose 50%. Results: Electrophoretic profiles of male’s and female’s venom pools were quite similar, with minor sex-based variation. Male venom showed higher LAAO, PLA2 and hemorrhagic activities, while female venom showed higher coagulant activity. On the other hand, the proteolytic activities did not show statistical differences between pools, although some individual variations were observed. Meanwhile, proteomic profile revealed 112 different protein compounds; of which 105 were common proteins of female’s and male’s venom pools and seven were unique to females. Despite individual variations, lethality of both pools showed similar values. Conclusion: Although differences between female and male venoms were observed, our results show that individual variations are significant even between siblings, highlighting that biological activities of venoms and its composition are influenced by other factors beyond gender. |
format |
Article in Journal/Newspaper |
author |
Daniela Miki Hatakeyama Lídia Jorge Tasima Cesar Adolfo Bravo-Tobar Caroline Serino-Silva Alexandre Keiji Tashima Caroline Fabri Bittencourt Rodrigues Weslei da Silva Aguiar Nathália da Costa Galizio Eduardo Oliveira Venancio de Lima Victor Koiti Kavazoi Juan David Gutierrez-Marín Iasmim Baptista de Farias Sávio Stefanini Sant’Anna Kathleen Fernandes Grego Karen de Morais-Zani Anita Mitico Tanaka-Azevedo |
author_facet |
Daniela Miki Hatakeyama Lídia Jorge Tasima Cesar Adolfo Bravo-Tobar Caroline Serino-Silva Alexandre Keiji Tashima Caroline Fabri Bittencourt Rodrigues Weslei da Silva Aguiar Nathália da Costa Galizio Eduardo Oliveira Venancio de Lima Victor Koiti Kavazoi Juan David Gutierrez-Marín Iasmim Baptista de Farias Sávio Stefanini Sant’Anna Kathleen Fernandes Grego Karen de Morais-Zani Anita Mitico Tanaka-Azevedo |
author_sort |
Daniela Miki Hatakeyama |
title |
Venom complexity of Bothrops atrox (common lancehead) siblings |
title_short |
Venom complexity of Bothrops atrox (common lancehead) siblings |
title_full |
Venom complexity of Bothrops atrox (common lancehead) siblings |
title_fullStr |
Venom complexity of Bothrops atrox (common lancehead) siblings |
title_full_unstemmed |
Venom complexity of Bothrops atrox (common lancehead) siblings |
title_sort |
venom complexity of bothrops atrox (common lancehead) siblings |
publisher |
SciELO |
publishDate |
2020 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2020-0018 https://doaj.org/article/9b835479f1b14d86be995d42e5bb0391 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 26 (2020) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100334&tlng=en http://www.scielo.br/pdf/jvatitd/v26/1678-9199-jvatitd-26-e20200018.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2020-0018 https://doaj.org/article/9b835479f1b14d86be995d42e5bb0391 |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2020-0018 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
26 |
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1766346194456412160 |