Gut microbiome of captive wolves is more similar to domestic dogs than wild wolves indicated by metagenomics study
Adaptation during the domestication from wolves (Canis lupus) to dogs (Canis lupus familiaris) is a debated ecological topic. Changes in food and environment are major divergences in the domestication of dogs. Gut microbes play an important role in animal adaptation to the food and environmental cha...
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Online Access: | https://doi.org/10.3389/fmicb.2022.1027188 https://doaj.org/article/9b6f28bbb76a4e5cac0360fcc793499a |
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ftdoajarticles:oai:doaj.org/article:9b6f28bbb76a4e5cac0360fcc793499a 2023-05-15T15:49:30+02:00 Gut microbiome of captive wolves is more similar to domestic dogs than wild wolves indicated by metagenomics study Lei Chen Mengyao Sun Di Xu Zenghao Gao Yuying Shi Shen Wang Yiping Zhou 2022-11-01T00:00:00Z https://doi.org/10.3389/fmicb.2022.1027188 https://doaj.org/article/9b6f28bbb76a4e5cac0360fcc793499a EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmicb.2022.1027188/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2022.1027188 https://doaj.org/article/9b6f28bbb76a4e5cac0360fcc793499a Frontiers in Microbiology, Vol 13 (2022) Canis lupus Canis lupus familiaris captivity gut microbiome environmental adaption Microbiology QR1-502 article 2022 ftdoajarticles https://doi.org/10.3389/fmicb.2022.1027188 2022-12-30T20:49:54Z Adaptation during the domestication from wolves (Canis lupus) to dogs (Canis lupus familiaris) is a debated ecological topic. Changes in food and environment are major divergences in the domestication of dogs. Gut microbes play an important role in animal adaptation to the food and environmental changes. In this study, shotgun sequencing was performed to compare the species diversity and functional diversity of gut microbes in wild wolves (group CLW, n = 3), captive wolves (group CLC, n = 4), and domestic dogs (group CLF, n = 4). The results found that Bacteroidetes, Firmicutes, Fusobacteria, Proteobacteria and Actinobacteria were the most abundant phyla and Bacteroides, Fusobacterium, Prevotella, Megamonas, Paraprevotella, Faecalibacterium, Clostridium were the most abundant genera in the gut of wolves and dogs. Groups CLW, CLC and CLF have shown significant difference in gut microbial species diversity and functional diversity. Bacteroides, Fusobacterium and Faecalibacterium were most abundant genera in groups CLW, CLC and CLF, respectively. Their abundance varied significantly among groups. Compared to the wild wolves, the intestinal microbiol genes of domestic dogs were significantly enriched in the carbohydrate metabolism pathway of KEGG database. One hundred and seventy-seven enzymes were detected with significantly higher abundance in group CLF than that in group CLW, and 49 enzymes showed extremely significant higher abundance in group CLF than that in group CLW (q < 0.01) base on the function abundance annotated in CAZy database. It is noteworthy that there were also significant differences in the abundance of 140 enzymes between groups CLC and CLW (q < 0.05). Clustering analysis based on both the species and the function abundance of intestinal microbiota all found that groups CLC and CLF clustered into one branch, while samples from group CLW clustered into the other branch. This result suggests that captive wolves are more similar to domestic dogs than wild wolves in both species composition ... Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles Frontiers in Microbiology 13 |
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topic |
Canis lupus Canis lupus familiaris captivity gut microbiome environmental adaption Microbiology QR1-502 |
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Canis lupus Canis lupus familiaris captivity gut microbiome environmental adaption Microbiology QR1-502 Lei Chen Mengyao Sun Di Xu Zenghao Gao Yuying Shi Shen Wang Yiping Zhou Gut microbiome of captive wolves is more similar to domestic dogs than wild wolves indicated by metagenomics study |
topic_facet |
Canis lupus Canis lupus familiaris captivity gut microbiome environmental adaption Microbiology QR1-502 |
description |
Adaptation during the domestication from wolves (Canis lupus) to dogs (Canis lupus familiaris) is a debated ecological topic. Changes in food and environment are major divergences in the domestication of dogs. Gut microbes play an important role in animal adaptation to the food and environmental changes. In this study, shotgun sequencing was performed to compare the species diversity and functional diversity of gut microbes in wild wolves (group CLW, n = 3), captive wolves (group CLC, n = 4), and domestic dogs (group CLF, n = 4). The results found that Bacteroidetes, Firmicutes, Fusobacteria, Proteobacteria and Actinobacteria were the most abundant phyla and Bacteroides, Fusobacterium, Prevotella, Megamonas, Paraprevotella, Faecalibacterium, Clostridium were the most abundant genera in the gut of wolves and dogs. Groups CLW, CLC and CLF have shown significant difference in gut microbial species diversity and functional diversity. Bacteroides, Fusobacterium and Faecalibacterium were most abundant genera in groups CLW, CLC and CLF, respectively. Their abundance varied significantly among groups. Compared to the wild wolves, the intestinal microbiol genes of domestic dogs were significantly enriched in the carbohydrate metabolism pathway of KEGG database. One hundred and seventy-seven enzymes were detected with significantly higher abundance in group CLF than that in group CLW, and 49 enzymes showed extremely significant higher abundance in group CLF than that in group CLW (q < 0.01) base on the function abundance annotated in CAZy database. It is noteworthy that there were also significant differences in the abundance of 140 enzymes between groups CLC and CLW (q < 0.05). Clustering analysis based on both the species and the function abundance of intestinal microbiota all found that groups CLC and CLF clustered into one branch, while samples from group CLW clustered into the other branch. This result suggests that captive wolves are more similar to domestic dogs than wild wolves in both species composition ... |
format |
Article in Journal/Newspaper |
author |
Lei Chen Mengyao Sun Di Xu Zenghao Gao Yuying Shi Shen Wang Yiping Zhou |
author_facet |
Lei Chen Mengyao Sun Di Xu Zenghao Gao Yuying Shi Shen Wang Yiping Zhou |
author_sort |
Lei Chen |
title |
Gut microbiome of captive wolves is more similar to domestic dogs than wild wolves indicated by metagenomics study |
title_short |
Gut microbiome of captive wolves is more similar to domestic dogs than wild wolves indicated by metagenomics study |
title_full |
Gut microbiome of captive wolves is more similar to domestic dogs than wild wolves indicated by metagenomics study |
title_fullStr |
Gut microbiome of captive wolves is more similar to domestic dogs than wild wolves indicated by metagenomics study |
title_full_unstemmed |
Gut microbiome of captive wolves is more similar to domestic dogs than wild wolves indicated by metagenomics study |
title_sort |
gut microbiome of captive wolves is more similar to domestic dogs than wild wolves indicated by metagenomics study |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmicb.2022.1027188 https://doaj.org/article/9b6f28bbb76a4e5cac0360fcc793499a |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Frontiers in Microbiology, Vol 13 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmicb.2022.1027188/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2022.1027188 https://doaj.org/article/9b6f28bbb76a4e5cac0360fcc793499a |
op_doi |
https://doi.org/10.3389/fmicb.2022.1027188 |
container_title |
Frontiers in Microbiology |
container_volume |
13 |
_version_ |
1766384540826206208 |