EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES
INTRODUCTION: Methicillin-Resistant Staphylococcus aureus (MRSA) presenting reduced susceptibility to vancomycin has been associated to therapeutic failure. Some methods used by clinical laboratories may not be sufficiently accurate to detect this phenotype, compromising results and the outcome of t...
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Universidade de São Paulo (USP)
2014
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Online Access: | https://doi.org/10.1590/S0036-46652014000600002 https://doaj.org/article/9b4e2e0b3b3b49c08e1e9677bea6de29 |
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ftdoajarticles:oai:doaj.org/article:9b4e2e0b3b3b49c08e1e9677bea6de29 2024-09-09T19:26:47+00:00 EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES Fernanda Cristina Possamai Rossatto Letícia Auler Proença Ana Paula Becker Alessandro Conrado de Oliveira Silveira Juliana Caierão Pedro Alves D'azevedo 2014-12-01T00:00:00Z https://doi.org/10.1590/S0036-46652014000600002 https://doaj.org/article/9b4e2e0b3b3b49c08e1e9677bea6de29 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652014000600469&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652014000600002 https://doaj.org/article/9b4e2e0b3b3b49c08e1e9677bea6de29 Revista do Instituto de Medicina Tropical de São Paulo, Vol 56, Iss 6, Pp 469-472 (2014) Methicillin-Resistant Staphylococcus aureus Vancomycin Minimal Inhibitory Concentration Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2014 ftdoajarticles https://doi.org/10.1590/S0036-46652014000600002 2024-08-05T17:49:30Z INTRODUCTION: Methicillin-Resistant Staphylococcus aureus (MRSA) presenting reduced susceptibility to vancomycin has been associated to therapeutic failure. Some methods used by clinical laboratories may not be sufficiently accurate to detect this phenotype, compromising results and the outcome of the patient. OBJECTIVES: To evaluate the performance of methods in the detection of vancomycin MIC values among clinical isolates of MRSA. MATERIAL AND METHODS: The Vancomycin Minimal Inhibitory Concentration was determined for 75 MRSA isolates from inpatients of Mãe de Deus Hospital, Porto Alegre, Brazil. The broth microdilution (BM) was used as the gold-standard technique, as well as the following methods: E-test® strips (BioMérieux), M.I.C.E® strips (Oxoid), PROBAC® commercial panel and the automated system MicroScan® (Siemens). Besides, the agar screening test was carried out with 3 µg/mL of vancomycin. RESULTS: All isolates presented MIC ≤ 2 µg/mL for BM. E-test® had higher concordance (40%) in terms of global agreement with the gold standard, and there was not statistical difference among E-test® and broth microdilution results. PROBAC® panels presented MICs, in general, lower than the gold-standard panels (58.66% major errors), while M.I.C.E.® MICs were higher (67.99% minor errors). CONCLUSIONS: For the population of MRSA in question, E-test® presented the best performance, although with a heterogeneous accuracy, depending on MIC values. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 56 6 469 472 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Methicillin-Resistant Staphylococcus aureus Vancomycin Minimal Inhibitory Concentration Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Methicillin-Resistant Staphylococcus aureus Vancomycin Minimal Inhibitory Concentration Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Fernanda Cristina Possamai Rossatto Letícia Auler Proença Ana Paula Becker Alessandro Conrado de Oliveira Silveira Juliana Caierão Pedro Alves D'azevedo EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES |
topic_facet |
Methicillin-Resistant Staphylococcus aureus Vancomycin Minimal Inhibitory Concentration Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
INTRODUCTION: Methicillin-Resistant Staphylococcus aureus (MRSA) presenting reduced susceptibility to vancomycin has been associated to therapeutic failure. Some methods used by clinical laboratories may not be sufficiently accurate to detect this phenotype, compromising results and the outcome of the patient. OBJECTIVES: To evaluate the performance of methods in the detection of vancomycin MIC values among clinical isolates of MRSA. MATERIAL AND METHODS: The Vancomycin Minimal Inhibitory Concentration was determined for 75 MRSA isolates from inpatients of Mãe de Deus Hospital, Porto Alegre, Brazil. The broth microdilution (BM) was used as the gold-standard technique, as well as the following methods: E-test® strips (BioMérieux), M.I.C.E® strips (Oxoid), PROBAC® commercial panel and the automated system MicroScan® (Siemens). Besides, the agar screening test was carried out with 3 µg/mL of vancomycin. RESULTS: All isolates presented MIC ≤ 2 µg/mL for BM. E-test® had higher concordance (40%) in terms of global agreement with the gold standard, and there was not statistical difference among E-test® and broth microdilution results. PROBAC® panels presented MICs, in general, lower than the gold-standard panels (58.66% major errors), while M.I.C.E.® MICs were higher (67.99% minor errors). CONCLUSIONS: For the population of MRSA in question, E-test® presented the best performance, although with a heterogeneous accuracy, depending on MIC values. |
format |
Article in Journal/Newspaper |
author |
Fernanda Cristina Possamai Rossatto Letícia Auler Proença Ana Paula Becker Alessandro Conrado de Oliveira Silveira Juliana Caierão Pedro Alves D'azevedo |
author_facet |
Fernanda Cristina Possamai Rossatto Letícia Auler Proença Ana Paula Becker Alessandro Conrado de Oliveira Silveira Juliana Caierão Pedro Alves D'azevedo |
author_sort |
Fernanda Cristina Possamai Rossatto |
title |
EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES |
title_short |
EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES |
title_full |
EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES |
title_fullStr |
EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES |
title_full_unstemmed |
EVALUATION OF METHODS IN DETECTING VANCOMYCIN MIC AMONG MRSA ISOLATES AND THE CHANGES IN ACCURACY RELATED TO DIFFERENT MIC VALUES |
title_sort |
evaluation of methods in detecting vancomycin mic among mrsa isolates and the changes in accuracy related to different mic values |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2014 |
url |
https://doi.org/10.1590/S0036-46652014000600002 https://doaj.org/article/9b4e2e0b3b3b49c08e1e9677bea6de29 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 56, Iss 6, Pp 469-472 (2014) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652014000600469&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652014000600002 https://doaj.org/article/9b4e2e0b3b3b49c08e1e9677bea6de29 |
op_doi |
https://doi.org/10.1590/S0036-46652014000600002 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
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56 |
container_issue |
6 |
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469 |
op_container_end_page |
472 |
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