CRISPR associated diversity within a population of Sulfolobus islandicus.
Predator-prey models for virus-host interactions predict that viruses will cause oscillations of microbial host densities due to an arms race between resistance and virulence. A new form of microbial resistance, CRISPRs (clustered regularly interspaced short palindromic repeats) are a rapidly evolvi...
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ftdoajarticles:oai:doaj.org/article:9b4016dc9de54bc59f3573350087188a 2023-05-15T16:59:19+02:00 CRISPR associated diversity within a population of Sulfolobus islandicus. Nicole L Held Alfa Herrera Hinsby Cadillo-Quiroz Rachel J Whitaker 2010-09-01T00:00:00Z https://doi.org/10.1371/journal.pone.0012988 https://doaj.org/article/9b4016dc9de54bc59f3573350087188a EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2946923?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0012988 https://doaj.org/article/9b4016dc9de54bc59f3573350087188a PLoS ONE, Vol 5, Iss 9 (2010) Medicine R Science Q article 2010 ftdoajarticles https://doi.org/10.1371/journal.pone.0012988 2022-12-31T08:17:32Z Predator-prey models for virus-host interactions predict that viruses will cause oscillations of microbial host densities due to an arms race between resistance and virulence. A new form of microbial resistance, CRISPRs (clustered regularly interspaced short palindromic repeats) are a rapidly evolving, sequence-specific immunity mechanism in which a short piece of invading viral DNA is inserted into the host's chromosome, thereby rendering the host resistant to further infection. Few studies have linked this form of resistance to population dynamics in natural microbial populations.We examined sequence diversity in 39 strains of the archeaon Sulfolobus islandicus from a single, isolated hot spring from Kamchatka, Russia to determine the effects of CRISPR immunity on microbial population dynamics. First, multiple housekeeping genetic markers identify a large clonal group of identical genotypes coexisting with a diverse set of rare genotypes. Second, the sequence-specific CRISPR spacer arrays split the large group of isolates into two very different groups and reveal extensive diversity and no evidence for dominance of a single clone within the population.The evenness of resistance genotypes found within this population of S. islandicus is indicative of a lack of strain dominance, in contrast to the prediction for a resistant strain in a simple predator-prey interaction. Based on evidence for the independent acquisition of resistant sequences, we hypothesize that CRISPR mediated clonal interference between resistant strains promotes and maintains diversity in this natural population. Article in Journal/Newspaper Kamchatka Directory of Open Access Journals: DOAJ Articles PLoS ONE 5 9 e12988 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Nicole L Held Alfa Herrera Hinsby Cadillo-Quiroz Rachel J Whitaker CRISPR associated diversity within a population of Sulfolobus islandicus. |
topic_facet |
Medicine R Science Q |
description |
Predator-prey models for virus-host interactions predict that viruses will cause oscillations of microbial host densities due to an arms race between resistance and virulence. A new form of microbial resistance, CRISPRs (clustered regularly interspaced short palindromic repeats) are a rapidly evolving, sequence-specific immunity mechanism in which a short piece of invading viral DNA is inserted into the host's chromosome, thereby rendering the host resistant to further infection. Few studies have linked this form of resistance to population dynamics in natural microbial populations.We examined sequence diversity in 39 strains of the archeaon Sulfolobus islandicus from a single, isolated hot spring from Kamchatka, Russia to determine the effects of CRISPR immunity on microbial population dynamics. First, multiple housekeeping genetic markers identify a large clonal group of identical genotypes coexisting with a diverse set of rare genotypes. Second, the sequence-specific CRISPR spacer arrays split the large group of isolates into two very different groups and reveal extensive diversity and no evidence for dominance of a single clone within the population.The evenness of resistance genotypes found within this population of S. islandicus is indicative of a lack of strain dominance, in contrast to the prediction for a resistant strain in a simple predator-prey interaction. Based on evidence for the independent acquisition of resistant sequences, we hypothesize that CRISPR mediated clonal interference between resistant strains promotes and maintains diversity in this natural population. |
format |
Article in Journal/Newspaper |
author |
Nicole L Held Alfa Herrera Hinsby Cadillo-Quiroz Rachel J Whitaker |
author_facet |
Nicole L Held Alfa Herrera Hinsby Cadillo-Quiroz Rachel J Whitaker |
author_sort |
Nicole L Held |
title |
CRISPR associated diversity within a population of Sulfolobus islandicus. |
title_short |
CRISPR associated diversity within a population of Sulfolobus islandicus. |
title_full |
CRISPR associated diversity within a population of Sulfolobus islandicus. |
title_fullStr |
CRISPR associated diversity within a population of Sulfolobus islandicus. |
title_full_unstemmed |
CRISPR associated diversity within a population of Sulfolobus islandicus. |
title_sort |
crispr associated diversity within a population of sulfolobus islandicus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pone.0012988 https://doaj.org/article/9b4016dc9de54bc59f3573350087188a |
genre |
Kamchatka |
genre_facet |
Kamchatka |
op_source |
PLoS ONE, Vol 5, Iss 9 (2010) |
op_relation |
http://europepmc.org/articles/PMC2946923?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0012988 https://doaj.org/article/9b4016dc9de54bc59f3573350087188a |
op_doi |
https://doi.org/10.1371/journal.pone.0012988 |
container_title |
PLoS ONE |
container_volume |
5 |
container_issue |
9 |
container_start_page |
e12988 |
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1766051565118947328 |