Porin from Marine Bacterium Marinomonas primoryensis KMM 3633 T : Isolation, Physico-Chemical Properties, and Functional Activity
Marinomonas primoryensis KMM 3633 T , extreme living marine bacterium was isolated from a sample of coastal sea ice in the Amursky Bay near Vladivostok, Russia. The goal of our investigation is to study outer membrane channels determining cell permeability. Porin from M. primoryensis KMM 3633 T (MpO...
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ftdoajarticles:oai:doaj.org/article:9aea5a212efb43bf934e0a5ca1ccf5e8 2023-05-15T18:18:32+02:00 Porin from Marine Bacterium Marinomonas primoryensis KMM 3633 T : Isolation, Physico-Chemical Properties, and Functional Activity Olga D. Novikova Valentina A. Khomenko Natalia Yu. Kim Galina N. Likhatskaya Lyudmila A. Romanenko Ekaterina I. Aksenova Marina S. Kunda Natalia N. Ryzhova Olga Yu. Portnyagina Tamara F. Solov’eva Olga L. Voronina 2020-07-01T00:00:00Z https://doi.org/10.3390/molecules25143131 https://doaj.org/article/9aea5a212efb43bf934e0a5ca1ccf5e8 EN eng MDPI AG https://www.mdpi.com/1420-3049/25/14/3131 https://doaj.org/toc/1420-3049 doi:10.3390/molecules25143131 1420-3049 https://doaj.org/article/9aea5a212efb43bf934e0a5ca1ccf5e8 Molecules, Vol 25, Iss 3131, p 3131 (2020) marine bacteria whole genome sequence porin amino acids composition bilayer lipid membrane pore-forming activity Organic chemistry QD241-441 article 2020 ftdoajarticles https://doi.org/10.3390/molecules25143131 2022-12-31T06:50:12Z Marinomonas primoryensis KMM 3633 T , extreme living marine bacterium was isolated from a sample of coastal sea ice in the Amursky Bay near Vladivostok, Russia. The goal of our investigation is to study outer membrane channels determining cell permeability. Porin from M. primoryensis KMM 3633 T (MpOmp) has been isolated and characterized. Amino acid analysis and whole genome sequencing were the sources of amino acid data of porin, identified as Porin_4 according to the conservative domain searching. The amino acid composition of MpOmp distinguished by high content of acidic amino acids and low content of sulfur-containing amino acids, but there are no tryptophan residues in its molecule. The native MpOmp existed as a trimer. The reconstitution of MpOmp into black lipid membranes demonstrated its ability to form ion channels whose conductivity depends on the electrolyte concentration. The spatial structure of MpOmp had features typical for the classical gram-negative porins. However, the oligomeric structure of isolated MpOmp was distinguished by very low stability: heat-modified monomer was already observed at 30 °C. The data obtained suggest the stabilizing role of lipids in the natural membrane of marine bacteria in the formation of the oligomeric structure of porin. Article in Journal/Newspaper Sea ice Directory of Open Access Journals: DOAJ Articles Molecules 25 14 3131 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
marine bacteria whole genome sequence porin amino acids composition bilayer lipid membrane pore-forming activity Organic chemistry QD241-441 |
spellingShingle |
marine bacteria whole genome sequence porin amino acids composition bilayer lipid membrane pore-forming activity Organic chemistry QD241-441 Olga D. Novikova Valentina A. Khomenko Natalia Yu. Kim Galina N. Likhatskaya Lyudmila A. Romanenko Ekaterina I. Aksenova Marina S. Kunda Natalia N. Ryzhova Olga Yu. Portnyagina Tamara F. Solov’eva Olga L. Voronina Porin from Marine Bacterium Marinomonas primoryensis KMM 3633 T : Isolation, Physico-Chemical Properties, and Functional Activity |
topic_facet |
marine bacteria whole genome sequence porin amino acids composition bilayer lipid membrane pore-forming activity Organic chemistry QD241-441 |
description |
Marinomonas primoryensis KMM 3633 T , extreme living marine bacterium was isolated from a sample of coastal sea ice in the Amursky Bay near Vladivostok, Russia. The goal of our investigation is to study outer membrane channels determining cell permeability. Porin from M. primoryensis KMM 3633 T (MpOmp) has been isolated and characterized. Amino acid analysis and whole genome sequencing were the sources of amino acid data of porin, identified as Porin_4 according to the conservative domain searching. The amino acid composition of MpOmp distinguished by high content of acidic amino acids and low content of sulfur-containing amino acids, but there are no tryptophan residues in its molecule. The native MpOmp existed as a trimer. The reconstitution of MpOmp into black lipid membranes demonstrated its ability to form ion channels whose conductivity depends on the electrolyte concentration. The spatial structure of MpOmp had features typical for the classical gram-negative porins. However, the oligomeric structure of isolated MpOmp was distinguished by very low stability: heat-modified monomer was already observed at 30 °C. The data obtained suggest the stabilizing role of lipids in the natural membrane of marine bacteria in the formation of the oligomeric structure of porin. |
format |
Article in Journal/Newspaper |
author |
Olga D. Novikova Valentina A. Khomenko Natalia Yu. Kim Galina N. Likhatskaya Lyudmila A. Romanenko Ekaterina I. Aksenova Marina S. Kunda Natalia N. Ryzhova Olga Yu. Portnyagina Tamara F. Solov’eva Olga L. Voronina |
author_facet |
Olga D. Novikova Valentina A. Khomenko Natalia Yu. Kim Galina N. Likhatskaya Lyudmila A. Romanenko Ekaterina I. Aksenova Marina S. Kunda Natalia N. Ryzhova Olga Yu. Portnyagina Tamara F. Solov’eva Olga L. Voronina |
author_sort |
Olga D. Novikova |
title |
Porin from Marine Bacterium Marinomonas primoryensis KMM 3633 T : Isolation, Physico-Chemical Properties, and Functional Activity |
title_short |
Porin from Marine Bacterium Marinomonas primoryensis KMM 3633 T : Isolation, Physico-Chemical Properties, and Functional Activity |
title_full |
Porin from Marine Bacterium Marinomonas primoryensis KMM 3633 T : Isolation, Physico-Chemical Properties, and Functional Activity |
title_fullStr |
Porin from Marine Bacterium Marinomonas primoryensis KMM 3633 T : Isolation, Physico-Chemical Properties, and Functional Activity |
title_full_unstemmed |
Porin from Marine Bacterium Marinomonas primoryensis KMM 3633 T : Isolation, Physico-Chemical Properties, and Functional Activity |
title_sort |
porin from marine bacterium marinomonas primoryensis kmm 3633 t : isolation, physico-chemical properties, and functional activity |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/molecules25143131 https://doaj.org/article/9aea5a212efb43bf934e0a5ca1ccf5e8 |
genre |
Sea ice |
genre_facet |
Sea ice |
op_source |
Molecules, Vol 25, Iss 3131, p 3131 (2020) |
op_relation |
https://www.mdpi.com/1420-3049/25/14/3131 https://doaj.org/toc/1420-3049 doi:10.3390/molecules25143131 1420-3049 https://doaj.org/article/9aea5a212efb43bf934e0a5ca1ccf5e8 |
op_doi |
https://doi.org/10.3390/molecules25143131 |
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Molecules |
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25 |
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14 |
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3131 |
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1766195136108167168 |