Archaea in the microbial community of the reindeer rumen in the Russian Arctic
Archaea is the least studied group of the reindeer rumen microbiocenosis. Although the functional load performed by this group of microorganisms in the rumen is large. Methane-forming archaea play a key role in the process of anaerobic decomposition of organic substances, the formation of methane. T...
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ftdoajarticles:oai:doaj.org/article:9ad5a83fe02f4cc0aecdd696d61d5d02 2023-05-15T15:00:36+02:00 Archaea in the microbial community of the reindeer rumen in the Russian Arctic Ilina Larisa Filippova Valentina Yildirim Elena Layshev Kasim 2020-01-01T00:00:00Z https://doi.org/10.1051/bioconf/20202700066 https://doaj.org/article/9ad5a83fe02f4cc0aecdd696d61d5d02 EN FR eng fre EDP Sciences https://www.bio-conferences.org/articles/bioconf/full_html/2020/11/bioconf_fies-20_00066/bioconf_fies-20_00066.html https://doaj.org/toc/2117-4458 2117-4458 doi:10.1051/bioconf/20202700066 https://doaj.org/article/9ad5a83fe02f4cc0aecdd696d61d5d02 BIO Web of Conferences, Vol 27, p 00066 (2020) Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991 article 2020 ftdoajarticles https://doi.org/10.1051/bioconf/20202700066 2022-12-31T04:04:41Z Archaea is the least studied group of the reindeer rumen microbiocenosis. Although the functional load performed by this group of microorganisms in the rumen is large. Methane-forming archaea play a key role in the process of anaerobic decomposition of organic substances, the formation of methane. This study for the first time analyzed the composition of the archaeal part of the microbial community of the reindeer rumen using the T-RFLP method from various regions of the Russian Arctic. As a result, it was found that according to the estimates of the number of archaea by quantitative PCR in the reindeer rumen in the winter-spring period, on average, 108 genomes/g of archaea were observed in individuals of the Yamalo-Nenets Autonomous District, and 109 genomes/in animals from the Nenets Autonomous District Archean. Thus, in the winter-spring period, a lower number of archaea in the rumen was observed in the Yamalo-Nenets Autonomous District. According to the results of the T-RFLP method, 44 to 134 phylotypes were detected in the archaeal community of the reindeer rumen, the Shannon index was 2.02–3.80. The lowest content (up to 11.10 %) of methanogenic archaea of the Methanomicrobia class (including the families Methanosarcinaceae and Methanocorpusculaceae) was revealed in the Nenets Autonomous District, while their presence in individuals of the Yamalo-Nenets Autonomous District reached 36.33 %. Interestingly, in adults of the Yamalo-Nenets and Nenets Autonomous Districts, a significant decrease in the representation of methanogenic archaea of the Methanomicrobia class was noted by 1.38 (P <0.05) and 2.70 times (P <0.01), respectively, compared with young individuals (up to 2 years). Article in Journal/Newspaper Arctic nenets Yamalo Nenets Directory of Open Access Journals: DOAJ Articles Arctic BIO Web of Conferences 27 00066 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English French |
topic |
Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991 |
spellingShingle |
Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991 Ilina Larisa Filippova Valentina Yildirim Elena Layshev Kasim Archaea in the microbial community of the reindeer rumen in the Russian Arctic |
topic_facet |
Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991 |
description |
Archaea is the least studied group of the reindeer rumen microbiocenosis. Although the functional load performed by this group of microorganisms in the rumen is large. Methane-forming archaea play a key role in the process of anaerobic decomposition of organic substances, the formation of methane. This study for the first time analyzed the composition of the archaeal part of the microbial community of the reindeer rumen using the T-RFLP method from various regions of the Russian Arctic. As a result, it was found that according to the estimates of the number of archaea by quantitative PCR in the reindeer rumen in the winter-spring period, on average, 108 genomes/g of archaea were observed in individuals of the Yamalo-Nenets Autonomous District, and 109 genomes/in animals from the Nenets Autonomous District Archean. Thus, in the winter-spring period, a lower number of archaea in the rumen was observed in the Yamalo-Nenets Autonomous District. According to the results of the T-RFLP method, 44 to 134 phylotypes were detected in the archaeal community of the reindeer rumen, the Shannon index was 2.02–3.80. The lowest content (up to 11.10 %) of methanogenic archaea of the Methanomicrobia class (including the families Methanosarcinaceae and Methanocorpusculaceae) was revealed in the Nenets Autonomous District, while their presence in individuals of the Yamalo-Nenets Autonomous District reached 36.33 %. Interestingly, in adults of the Yamalo-Nenets and Nenets Autonomous Districts, a significant decrease in the representation of methanogenic archaea of the Methanomicrobia class was noted by 1.38 (P <0.05) and 2.70 times (P <0.01), respectively, compared with young individuals (up to 2 years). |
format |
Article in Journal/Newspaper |
author |
Ilina Larisa Filippova Valentina Yildirim Elena Layshev Kasim |
author_facet |
Ilina Larisa Filippova Valentina Yildirim Elena Layshev Kasim |
author_sort |
Ilina Larisa |
title |
Archaea in the microbial community of the reindeer rumen in the Russian Arctic |
title_short |
Archaea in the microbial community of the reindeer rumen in the Russian Arctic |
title_full |
Archaea in the microbial community of the reindeer rumen in the Russian Arctic |
title_fullStr |
Archaea in the microbial community of the reindeer rumen in the Russian Arctic |
title_full_unstemmed |
Archaea in the microbial community of the reindeer rumen in the Russian Arctic |
title_sort |
archaea in the microbial community of the reindeer rumen in the russian arctic |
publisher |
EDP Sciences |
publishDate |
2020 |
url |
https://doi.org/10.1051/bioconf/20202700066 https://doaj.org/article/9ad5a83fe02f4cc0aecdd696d61d5d02 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic nenets Yamalo Nenets |
genre_facet |
Arctic nenets Yamalo Nenets |
op_source |
BIO Web of Conferences, Vol 27, p 00066 (2020) |
op_relation |
https://www.bio-conferences.org/articles/bioconf/full_html/2020/11/bioconf_fies-20_00066/bioconf_fies-20_00066.html https://doaj.org/toc/2117-4458 2117-4458 doi:10.1051/bioconf/20202700066 https://doaj.org/article/9ad5a83fe02f4cc0aecdd696d61d5d02 |
op_doi |
https://doi.org/10.1051/bioconf/20202700066 |
container_title |
BIO Web of Conferences |
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27 |
container_start_page |
00066 |
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1766332685624541184 |