Production of anti-Cryptosporidium polyclonal antibodies and standardization of direct immunofluorescence for detecting oocysts in water
INTRODUCTION: The production of anti-Cryptosporidium polyclonal antibodies and its use in direct immunofluorescence assays to determine the presence of Cryptosporidium in water are described in the present work. METHODS: Two rabbits were immunized with soluble and particulate antigens from purified...
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Sociedade Brasileira de Medicina Tropical (SBMT)
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ftdoajarticles:oai:doaj.org/article:9ac2a2077e6b4b5b845a5c8873617e8a 2023-05-15T15:05:09+02:00 Production of anti-Cryptosporidium polyclonal antibodies and standardization of direct immunofluorescence for detecting oocysts in water Silvia Cristina Osaki Adriana Oliveira Costa Ludmilla Della Coletta Troiano Ernesto Renato Kruger Juliana Tracz Pereira Nelson Luis Mello Fernandes Márcia Benedita de Oliveira Silva Vanete Thomaz Soccol 2011-10-01T00:00:00Z https://doi.org/10.1590/S0037-86822011000500011 https://doaj.org/article/9ac2a2077e6b4b5b845a5c8873617e8a EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000500011&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822011000500011 https://doaj.org/article/9ac2a2077e6b4b5b845a5c8873617e8a Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 5, Pp 587-590 (2011) Anticorpos policlonais Imunofluorescência direta Oocistos Água Arctic medicine. Tropical medicine RC955-962 article 2011 ftdoajarticles https://doi.org/10.1590/S0037-86822011000500011 2022-12-30T21:37:26Z INTRODUCTION: The production of anti-Cryptosporidium polyclonal antibodies and its use in direct immunofluorescence assays to determine the presence of Cryptosporidium in water are described in the present work. METHODS: Two rabbits were immunized with soluble and particulate antigens from purified Cryptosporidium oocysts. The sera produced were prepared for immunoglobulin G extraction, which were then purified and conjugated with fluorescein isothiocyanate (FITC). Slides containing known amounts of oocysts were prepared to determine the sensitivity of the technique. To test the specificity, slides containing Giardia duodenalis cysts were prepared. RESULTS: The conjugate was successfully used in water samples experimentally contaminated with Cryptosporidium oocysts, and it was possible to detect up to five oocysts/spot, corresponding to contamination of 250 oocysts/mL. CONCLUSIONS: The three immunizations performed in the rabbits were enough to produce antibodies against Cryptosporidium, the standard direct immunofluorescence assay permitted the detection of five oocysts in 20% of the samples, and no cross-reaction with Giardia duodenalis cysts occurred. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 44 5 587 590 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Anticorpos policlonais Imunofluorescência direta Oocistos Água Arctic medicine. Tropical medicine RC955-962 |
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Anticorpos policlonais Imunofluorescência direta Oocistos Água Arctic medicine. Tropical medicine RC955-962 Silvia Cristina Osaki Adriana Oliveira Costa Ludmilla Della Coletta Troiano Ernesto Renato Kruger Juliana Tracz Pereira Nelson Luis Mello Fernandes Márcia Benedita de Oliveira Silva Vanete Thomaz Soccol Production of anti-Cryptosporidium polyclonal antibodies and standardization of direct immunofluorescence for detecting oocysts in water |
topic_facet |
Anticorpos policlonais Imunofluorescência direta Oocistos Água Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: The production of anti-Cryptosporidium polyclonal antibodies and its use in direct immunofluorescence assays to determine the presence of Cryptosporidium in water are described in the present work. METHODS: Two rabbits were immunized with soluble and particulate antigens from purified Cryptosporidium oocysts. The sera produced were prepared for immunoglobulin G extraction, which were then purified and conjugated with fluorescein isothiocyanate (FITC). Slides containing known amounts of oocysts were prepared to determine the sensitivity of the technique. To test the specificity, slides containing Giardia duodenalis cysts were prepared. RESULTS: The conjugate was successfully used in water samples experimentally contaminated with Cryptosporidium oocysts, and it was possible to detect up to five oocysts/spot, corresponding to contamination of 250 oocysts/mL. CONCLUSIONS: The three immunizations performed in the rabbits were enough to produce antibodies against Cryptosporidium, the standard direct immunofluorescence assay permitted the detection of five oocysts in 20% of the samples, and no cross-reaction with Giardia duodenalis cysts occurred. |
format |
Article in Journal/Newspaper |
author |
Silvia Cristina Osaki Adriana Oliveira Costa Ludmilla Della Coletta Troiano Ernesto Renato Kruger Juliana Tracz Pereira Nelson Luis Mello Fernandes Márcia Benedita de Oliveira Silva Vanete Thomaz Soccol |
author_facet |
Silvia Cristina Osaki Adriana Oliveira Costa Ludmilla Della Coletta Troiano Ernesto Renato Kruger Juliana Tracz Pereira Nelson Luis Mello Fernandes Márcia Benedita de Oliveira Silva Vanete Thomaz Soccol |
author_sort |
Silvia Cristina Osaki |
title |
Production of anti-Cryptosporidium polyclonal antibodies and standardization of direct immunofluorescence for detecting oocysts in water |
title_short |
Production of anti-Cryptosporidium polyclonal antibodies and standardization of direct immunofluorescence for detecting oocysts in water |
title_full |
Production of anti-Cryptosporidium polyclonal antibodies and standardization of direct immunofluorescence for detecting oocysts in water |
title_fullStr |
Production of anti-Cryptosporidium polyclonal antibodies and standardization of direct immunofluorescence for detecting oocysts in water |
title_full_unstemmed |
Production of anti-Cryptosporidium polyclonal antibodies and standardization of direct immunofluorescence for detecting oocysts in water |
title_sort |
production of anti-cryptosporidium polyclonal antibodies and standardization of direct immunofluorescence for detecting oocysts in water |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2011 |
url |
https://doi.org/10.1590/S0037-86822011000500011 https://doaj.org/article/9ac2a2077e6b4b5b845a5c8873617e8a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 5, Pp 587-590 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000500011&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822011000500011 https://doaj.org/article/9ac2a2077e6b4b5b845a5c8873617e8a |
op_doi |
https://doi.org/10.1590/S0037-86822011000500011 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
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44 |
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5 |
container_start_page |
587 |
op_container_end_page |
590 |
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