Diverging humoral and cellular immune responses due to Omicron—a national study from the Faroe Islands
ABSTRACT Immunity following infection and vaccination with the SARS-CoV-2 Omicron variant is poorly understood. The aim was to investigate immunity assessed with antibody response, neutralizing antibodies (NAbs), and IFN-γ release under different scenarios: in vaccinated and unvaccinated individuals...
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American Society for Microbiology
2023
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ftdoajarticles:oai:doaj.org/article:9a913f5af0834ead81f1c063d8b4f943 2024-01-14T10:06:43+01:00 Diverging humoral and cellular immune responses due to Omicron—a national study from the Faroe Islands Maria Skaalum Petersen Laura Pérez-Alós Sunnvør K. í Kongsstovu Eina Hansen Eliasen Cecilie Bo Hansen Sólrun Larsen Jóhanna Ljósá Hansen Rafael Bayarri-Olmos Jógvan Páll Fjallsbak Pál Weihe Peter Garred 2023-12-01T00:00:00Z https://doi.org/10.1128/spectrum.00865-23 https://doaj.org/article/9a913f5af0834ead81f1c063d8b4f943 EN eng American Society for Microbiology https://journals.asm.org/doi/10.1128/spectrum.00865-23 https://doaj.org/toc/2165-0497 doi:10.1128/spectrum.00865-23 2165-0497 https://doaj.org/article/9a913f5af0834ead81f1c063d8b4f943 Microbiology Spectrum, Vol 11, Iss 6 (2023) humoral immune response cellular immune response Omicron Faroe Islands Microbiology QR1-502 article 2023 ftdoajarticles https://doi.org/10.1128/spectrum.00865-23 2023-12-17T01:44:02Z ABSTRACT Immunity following infection and vaccination with the SARS-CoV-2 Omicron variant is poorly understood. The aim was to investigate immunity assessed with antibody response, neutralizing antibodies (NAbs), and IFN-γ release under different scenarios: in vaccinated and unvaccinated individuals with and without SARS-CoV-2 infection with the Omicron variant. This nationwide single-center study was conducted between January and March 2022, where all convalescent individuals were infected with the Omicron variant and included six study groups: unvaccinated-naïve, unvaccinated convalescent, vaccinated-naïve (second dose), vaccinated-naïve (third dose), vaccinated convalescent (second dose), and vaccinated convalescent (third dose). Antibody responses were assessed by determining receptor binding domain-specific antibodies and NAbs levels in serum, and IgG in saliva. T-cell responses in whole blood were measured as IFN-γ levels released after stimulation with spike peptides. We found that the humoral response against the spike protein was higher among vaccinated-naïve than unvaccinated convalescent. Unvaccinated with and without infection had comparable low humoral responses, while those vaccinated with a second or third dose, independent of infection status, had increasingly higher levels. Only 22% of the unvaccinated convalescent individuals mounted consistent detectable humoral responses following Omicron infection. However, 98% had spike peptide T-cell responses assessed by IFN-γ release. In conclusion, primary Omicron infection mounts a low humoral immune response, significantly enhanced by prior vaccination. Omicron infection induced a robust T-cell response in both unvaccinated and vaccinated, demonstrating that the evasive immune potential of primary Omicron infection affects humoral immunity more significantly than T-cell immunity. IMPORTANCE The immunity following infection and vaccination with the SARS-CoV-2 Omicron variant is poorly understood. We investigated immunity assessed with antibody and ... Article in Journal/Newspaper Faroe Islands Directory of Open Access Journals: DOAJ Articles Faroe Islands The Spike ENVELOPE(-37.317,-37.317,-54.017,-54.017) Microbiology Spectrum 11 6 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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humoral immune response cellular immune response Omicron Faroe Islands Microbiology QR1-502 |
spellingShingle |
humoral immune response cellular immune response Omicron Faroe Islands Microbiology QR1-502 Maria Skaalum Petersen Laura Pérez-Alós Sunnvør K. í Kongsstovu Eina Hansen Eliasen Cecilie Bo Hansen Sólrun Larsen Jóhanna Ljósá Hansen Rafael Bayarri-Olmos Jógvan Páll Fjallsbak Pál Weihe Peter Garred Diverging humoral and cellular immune responses due to Omicron—a national study from the Faroe Islands |
topic_facet |
humoral immune response cellular immune response Omicron Faroe Islands Microbiology QR1-502 |
description |
ABSTRACT Immunity following infection and vaccination with the SARS-CoV-2 Omicron variant is poorly understood. The aim was to investigate immunity assessed with antibody response, neutralizing antibodies (NAbs), and IFN-γ release under different scenarios: in vaccinated and unvaccinated individuals with and without SARS-CoV-2 infection with the Omicron variant. This nationwide single-center study was conducted between January and March 2022, where all convalescent individuals were infected with the Omicron variant and included six study groups: unvaccinated-naïve, unvaccinated convalescent, vaccinated-naïve (second dose), vaccinated-naïve (third dose), vaccinated convalescent (second dose), and vaccinated convalescent (third dose). Antibody responses were assessed by determining receptor binding domain-specific antibodies and NAbs levels in serum, and IgG in saliva. T-cell responses in whole blood were measured as IFN-γ levels released after stimulation with spike peptides. We found that the humoral response against the spike protein was higher among vaccinated-naïve than unvaccinated convalescent. Unvaccinated with and without infection had comparable low humoral responses, while those vaccinated with a second or third dose, independent of infection status, had increasingly higher levels. Only 22% of the unvaccinated convalescent individuals mounted consistent detectable humoral responses following Omicron infection. However, 98% had spike peptide T-cell responses assessed by IFN-γ release. In conclusion, primary Omicron infection mounts a low humoral immune response, significantly enhanced by prior vaccination. Omicron infection induced a robust T-cell response in both unvaccinated and vaccinated, demonstrating that the evasive immune potential of primary Omicron infection affects humoral immunity more significantly than T-cell immunity. IMPORTANCE The immunity following infection and vaccination with the SARS-CoV-2 Omicron variant is poorly understood. We investigated immunity assessed with antibody and ... |
format |
Article in Journal/Newspaper |
author |
Maria Skaalum Petersen Laura Pérez-Alós Sunnvør K. í Kongsstovu Eina Hansen Eliasen Cecilie Bo Hansen Sólrun Larsen Jóhanna Ljósá Hansen Rafael Bayarri-Olmos Jógvan Páll Fjallsbak Pál Weihe Peter Garred |
author_facet |
Maria Skaalum Petersen Laura Pérez-Alós Sunnvør K. í Kongsstovu Eina Hansen Eliasen Cecilie Bo Hansen Sólrun Larsen Jóhanna Ljósá Hansen Rafael Bayarri-Olmos Jógvan Páll Fjallsbak Pál Weihe Peter Garred |
author_sort |
Maria Skaalum Petersen |
title |
Diverging humoral and cellular immune responses due to Omicron—a national study from the Faroe Islands |
title_short |
Diverging humoral and cellular immune responses due to Omicron—a national study from the Faroe Islands |
title_full |
Diverging humoral and cellular immune responses due to Omicron—a national study from the Faroe Islands |
title_fullStr |
Diverging humoral and cellular immune responses due to Omicron—a national study from the Faroe Islands |
title_full_unstemmed |
Diverging humoral and cellular immune responses due to Omicron—a national study from the Faroe Islands |
title_sort |
diverging humoral and cellular immune responses due to omicron—a national study from the faroe islands |
publisher |
American Society for Microbiology |
publishDate |
2023 |
url |
https://doi.org/10.1128/spectrum.00865-23 https://doaj.org/article/9a913f5af0834ead81f1c063d8b4f943 |
long_lat |
ENVELOPE(-37.317,-37.317,-54.017,-54.017) |
geographic |
Faroe Islands The Spike |
geographic_facet |
Faroe Islands The Spike |
genre |
Faroe Islands |
genre_facet |
Faroe Islands |
op_source |
Microbiology Spectrum, Vol 11, Iss 6 (2023) |
op_relation |
https://journals.asm.org/doi/10.1128/spectrum.00865-23 https://doaj.org/toc/2165-0497 doi:10.1128/spectrum.00865-23 2165-0497 https://doaj.org/article/9a913f5af0834ead81f1c063d8b4f943 |
op_doi |
https://doi.org/10.1128/spectrum.00865-23 |
container_title |
Microbiology Spectrum |
container_volume |
11 |
container_issue |
6 |
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1788061167332098048 |